LOCUS AK297005 2165 bp mRNA linear HUM 24-JUL-2008
DEFINITION Homo sapiens cDNA FLJ58121 complete cds, highly similar to
Sphingosine 1-phosphate receptor Edg-1.
ACCESSION AK297005
VERSION AK297005.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2165)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..2165
/clone="D3OST1000360"
/clone_lib="D3OST1"
/db_xref="H-InvDB:HIT000491629"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from CD34+ cells after 3-days ODF induction."
/note="primary culture, CD34+ cells"
/organism="Homo sapiens"
/tissue_type="cord blood"
CDS 253..1605
/codon_start=1
/note="highly similar to Sphingosine 1-phosphate receptor
Edg-1"
/protein_id="BAG59541.1"
/transl_table=1
/translation="MGPTSVPLVKAHRSSVSDYVNYDIIVRHYNYTGKLNISADKENS
IKLTSVVFILICCFIILENIFVLLTIWKTKKFHRPMYYFIGNLALSDLLAGVAYTANL
LLSGATTYKLTPAQWFLREGSMFVALSASVFSLLAIAIERYITMLKMKLHNGSNNFRL
FLLISACWVISLILGGLPIMGWNCISALSSCSTVLPLYHKHYILFCTTVFTLLLLSIV
ILYCRIYSLVRTRSRRLTFRKNISKASRSSEKSLALLKTVIIVLSVFIACWAPLFILL
LLDVGCKVKTCDILFRAEYFLVLAVLNSGTNPIIYTLTNKETLLANSSDPSSPAWNSA
AANRTIPPTPRKTKGTTQRPLCLLETSTLLPRTGSCPPTGSALYLVAGHPSVWKKISG
LRLLPGRSCCKPEGGRGRIRTAWWCRVLVGRVSSCEQCTGKGGDQVPAWNIFSTPLEL
"
BASE COUNT 480 a 613 c 526 g 546 t
ORIGIN
1 gacttcgcgt agcaggcagg gaactggccg cggcgagcgg gactggccat tggagtgctc
61 cgctgcggag ggaggggacc ccgactcgag ctgcggtttc cgaggccctc tccagccaag
121 gaaaagctac acaaaaagcc tggatcactc atcgaaccac ccctgaagcc agtgaaggct
181 ctctcgcctc gccctctagc gttcgtctgg agtagcgcca ccccggcttc ctggggacac
241 aggtttggca ccatggggcc caccagcgtc ccgctggtca aggcccaccg cagctcggtc
301 tctgactacg tcaactatga tatcatcgtc cggcattaca actacacggg aaagctgaat
361 atcagcgcgg acaaggagaa cagcattaaa ctgacctcgg tggtgttcat tctcatctgc
421 tgctttatca tcctggagaa catctttgtc ttgctgacca tttggaaaac caagaaattc
481 caccgaccca tgtactattt tattggcaat ctggccctct cagacctgtt ggcaggagta
541 gcctacacag ctaacctgct cttgtctggg gccaccacct acaagctcac tcccgcccag
601 tggtttctgc gggaagggag tatgtttgtg gccctgtcag cctccgtgtt cagtctcctc
661 gccatcgcca ttgagcgcta tatcacaatg ctgaaaatga aactccacaa cgggagcaat
721 aacttccgcc tcttcctgct aatcagcgcc tgctgggtca tctccctcat cctgggtggc
781 ctgcctatca tgggctggaa ctgcatcagt gcgctgtcca gctgctccac cgtgctgccg
841 ctctaccaca agcactatat cctcttctgc accacggtct tcactctgct tctgctctcc
901 atcgtcattc tgtactgcag aatctactcc ttggtcagga ctcggagccg ccgcctgacg
961 ttccgcaaga acatttccaa ggccagccgc agctctgaga agtcgctggc gctgctcaag
1021 accgtaatta tcgtcctgag cgtcttcatc gcctgctggg caccgctctt catcctgctc
1081 ctgctggatg tgggctgcaa ggtgaagacc tgtgacatcc tcttcagagc ggagtacttc
1141 ctggtgttag ctgtgctcaa ctccggcacc aaccccatca tttacactct gaccaacaag
1201 gagactctgc tggcaaattc aagcgaccca tcatcgccgg catggaattc agccgcagca
1261 aatcggacaa ttcctcccac ccccagaaag acgaagggga caacccagag accattatgt
1321 cttctggaaa cgtcaactct tcttcctaga actggtagct gtccacccac cggaagcgct
1381 ctttacttgg tcgctggcca ccccagtgtt tggaaaaaaa tctctgggct tcgactgctg
1441 ccagggagga gctgctgcaa gccagaggga ggaaggggga gaatacgaac agcctggtgg
1501 tgtcgggtgt tggtgggtag agttagttcc tgtgaacaat gcactgggaa gggtggagat
1561 caggtcccgg cctggaatat attttctacc cccctggagc tttgattttg cactgagcca
1621 aaggtctagc attgtcaagc tcctaaaggg ttcatttggc ccctcctcaa agactaatgt
1681 ccccatgtga aagcgtctct ttgtctggag ctttgaggag atgttttcct tcactttagt
1741 ttcaaaccca agtgagtgtg tgcacttctg cttctttagg gatgccctgt acatcccaca
1801 ccccaccctc ccttcccttc atacccctcc tcaacgttct tttactttat actttaacta
1861 cctgagagtt atcagagctg gggttgtgga atgatcgatc atctatagca aataggctat
1921 gttgagtacg taggctgtgg gaagatgaag atggtttgga ggtgtaaaac aatgtccttc
1981 gctgaggcca aagtttccat gtaagcggga tccgtttttt ggaatttggt tgaagtcact
2041 ttgatttctt taaaaaacat cttttcaatg aaatgtgtta ccatttcata tccattgaag
2101 ccggaatctg cataaggaag cccactttat ctaaatgata ttagccagga tccttggtgt
2161 cctag
//