LOCUS AK296961 2606 bp mRNA linear HUM 31-JUL-2008
DEFINITION Homo sapiens cDNA FLJ56375 complete cds, highly similar to
Mitogen-activated protein kinase kinase kinase 13 (EC 2.7.11.25).
ACCESSION AK296961
VERSION AK296961.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2606)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..2606
/clone="CTONG3004706"
/clone_lib="CTONG3"
/db_xref="H-InvDB:HIT000491585"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="tumor tissue"
/organism="Homo sapiens"
/tissue_type="tongue, tumor tissue"
CDS 335..2554
/codon_start=1
/note="highly similar to Mitogen-activated protein kinase
kinase kinase 13 (EC 2.7.11.25)"
/protein_id="BAG59505.1"
/transl_table=1
/translation="MANFQEHLSCSSSPHLPFSESKTFNGLQDELTAMGNHPSPKLLE
DQQEKGMVRTELIESVHSPVTTTVLTSVSEDSRDQFENSVLQLREHDESETAVSQGNS
NTVGGESTSGTEDIKIQFSRSGSGSGGFLEGLFGCLRPVWNIIGKAYSTDYKLQQQDT
WEVPFEEISELQWLGSGAQGAVFLGKFRAEEVAIKKVREQNETDIKHLRKLKHPNIIA
FKGVCTQAPCYCIIMEYCAHGQLYEVLRAGRKITPRLLVDWSTGIASGMDYLHLHKII
HRDLKSPNVLVTHTDAVKISDFGTSKELSDKSTKMSFAGTVAWMAPEVIRNEPVSEKV
DIWSFGVVLWELLTGEIPYKDVDSSAIIWGVGSNSLHLPVPSTCPDGFKILMKQTWQS
KPRNRPSFRQTLMHLDIASADVLATPQETYFKSQAEWREEVKKHFEKIKSEGTCIHRL
DEELIRRRREELRHALDIREHYERKLERANNLYMELSAIMLQLEMREKELIKREQAVE
KKYPGTYKRHPVRPIIHPNAMEKLMKRKGVPHKSGMQTKRPDLLRSEGIPTTEVAPTA
SPLSGSPKMSTSSSKSRYRSKPRHRRGNSRGSHSDFAAILKNQPAQENSPHPTYLHQA
QSQYPSLHHHNSLQQQYQQPPPAMSQSHHPRLNMHGQDIATCANNLRYFGPAAALRSP
LSNHAQRQLPGSSPDLISTAMAADCWRSSEPDKGQAGPWGCCQADAYDPCLQCRPEQ"
BASE COUNT 724 a 649 c 642 g 591 t
ORIGIN
1 ccctcttttt tttttcatgg cacacaccac agcgaagtct gtgcggaatc ctaaaccagc
61 ccaatttaca tccattcatg aatctgtgac gtcagcaagc ctttgggctc ctttgcggtg
121 ggctggagga ttgtgtgggt ggaacccccc tcccctttat ttttccaatt ctgcaaggct
181 tttaaaattc accttacatc ttttcaaagc aagaaaatgg aacagcatgt gtaggaattc
241 ttcgttgttg ttttggagcc ctctcttaag tcagaactct gtcccaaaaa tcttctgagt
301 gtcatctcag gactttggtt atactcatgg cacgatggcc aactttcagg agcacctgag
361 ctgctcctct tctccacact tacccttcag tgaaagcaaa accttcaatg gactacaaga
421 tgagctcaca gctatgggga accacccttc tcccaagctg ctcgaggacc agcaggaaaa
481 ggggatggta cgaacagagc taatcgagag cgtgcacagc cccgtcacca caacagtgtt
541 gacgagcgta agtgaggatt ccagggacca gtttgagaac agcgtccttc agctaaggga
601 acacgatgaa tcagagacgg cggtgtctca ggggaacagc aacacggtgg gcggagagag
661 cacaagtgga actgaagaca taaagattca gttcagcagg tcaggcagtg gcagtggtgg
721 gtttcttgaa ggactatttg gatgcttaag gcctgtatgg aatatcattg ggaaggcata
781 ttccactgat tacaaattgc agcagcaaga tacttgggaa gtgccatttg aggagatctc
841 agagctgcag tggctgggta gtggagccca aggagcggtc ttcttgggca agttccgggc
901 ggaagaggtg gccatcaaga aagtgagaga acagaatgag acggatatca agcatttgag
961 gaagttgaag caccctaaca tcatcgcatt caagggtgtt tgtactcagg ccccatgtta
1021 ttgtattatc atggaatact gtgcccatgg acaactctac gaggtcttac gagctggcag
1081 gaagatcaca cctcgattgc tagtagactg gtccacagga attgcaagtg gaatggatta
1141 tttgcacctc cataaaatta ttcatcgtga tctcaaatca cctaatgttt tagtgaccca
1201 cacagatgcg gtaaaaattt cagattttgg tacgtctaag gaactcagtg acaaaagtac
1261 caagatgtca tttgctggca cggtcgcatg gatggcgcca gaggtgatac ggaatgaacc
1321 tgtttctgaa aaagttgata tatggtcttt tggagtggtg ctttgggagc tgctgacagg
1381 agagatccct tacaaagatg tagattcttc agccattatc tggggtgttg gaagcaacag
1441 cctccacctt ccagttcctt ccacttgccc tgatggattc aaaatcctta tgaaacagac
1501 gtggcagagt aaacctcgaa accgaccttc ttttcggcag acactcatgc atttagacat
1561 tgcctctgca gatgtacttg ccaccccaca agaaacttac ttcaagtctc aggctgaatg
1621 gagagaagaa gtgaaaaaac attttgagaa gatcaaaagt gaaggaactt gtatacaccg
1681 gttagatgaa gaactgattc gaaggcgcag agaagagctc aggcatgcgc tggatattcg
1741 tgaacactat gagcggaagc ttgagcgggc gaataattta tacatggaat tgagtgccat
1801 catgctgcag ctagaaatgc gggagaagga gctcattaag cgtgagcaag cagtggaaaa
1861 gaagtatcct gggacctaca aacgacaccc tgttcgtcct atcatccatc ccaatgccat
1921 ggagaaactc atgaaaagga aaggagtgcc tcacaaatct gggatgcaga ccaaacggcc
1981 agacttgttg agatcagaag ggatccccac cacagaagtg gctcccactg catccccttt
2041 gtccggaagt cccaaaatgt ccacttctag cagcaagagc cgatatcgaa gcaaaccacg
2101 ccaccgccga gggaatagca gaggcagcca tagtgacttt gccgcaatct tgaaaaacca
2161 gccagcccag gaaaattcac cccatcccac ttacctgcac caagctcaat cccaataccc
2221 ttctcttcat caccataatt ctctgcagca gcaataccag cagccccctc ctgccatgtc
2281 ccagagtcac catcccagac tcaatatgca cggacaggac atagcaacct gcgccaacaa
2341 cctgaggtat ttcggcccag cagcagccct gcggagccca ctcagcaacc atgctcagag
2401 acagctgccc ggctcgagcc ctgacctcat ctccacagcc atggctgcag actgctggag
2461 aagttctgag cctgacaagg gccaagctgg tccctggggc tgttgccagg ctgacgctta
2521 tgacccctgc cttcagtgca ggccagaaca gtaggggtcc ttagacatac cctctgctga
2581 gccagtgggg aggagccctg accttt
//