LOCUS AK296919 2425 bp mRNA linear HUM 21-JAN-2009
DEFINITION Homo sapiens cDNA FLJ56102 complete cds, highly similar to Homo
sapiens calpastatin (CAST), transcript variant 8, mRNA.
ACCESSION AK296919
VERSION AK296919.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2425)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..2425
/clone="CTONG2028451"
/clone_lib="CTONG2"
/db_xref="H-InvDB:HIT000491543"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="tumor tissue"
/organism="Homo sapiens"
/tissue_type="tongue, tumor tissue"
CDS 85..2349
/codon_start=1
/note="highly similar to Homo sapiens calpastatin (CAST),
transcript variant 8, mRNA"
/protein_id="BAH12454.1"
/transl_table=1
/translation="MAFASWWYKTHVSEKTSESPSKPGEKKGSDEKKAASLGSSQSSR
TYAGGTASATKVSASSGATSKSSSMNPTETKAVKTEPEKKSQSTKLSVVHEKKSQEGK
PKEHTEPKSLPKQASDTGSNDAHNKKAVSRSAEQQPSEKSTEPKTKPQDMISAGGESV
AGITAISGKPGDKKKEKKSLTPAVPVESKPDKPSGKSGMDAALDDLIDTLGGPEETEE
ENTTYTGPEVSDPMSSTYIEELGKREVTIPPKYRELLAKKEGITGPPADSSKPIGPDD
AIDALSSDFTCGSPTAAGKKTEKEESTEVLKAQSAGTVRSAAPPQEKKRKVEKDTMSD
QALEALSASLGTRQAEPELDLRSIKEVDEAKAKEEKLEKCGEDDETIPSEYRLKPATD
KDGKPLLPEPEEKPKPRSESELIDELSEDFDRSECKEKPSKPTEKTEESKAAAPAPVS
EAVCRTSMCSIQSAPPEPATLKGTVPDDAVEALADSLGKKEADPEDGKPVMDKVKEKA
KEEDREKLGEKEETIPPDYRLEEVKDKDGKPLLPKESKEQLPPMSEDFLLDALSEDFS
GPQNASSLKFEDAKLAAAISEVVSQTPASTTQAGAPPRDTSQSDKDLDDALDKLSDSL
GQRQPDPDENKPMEDKVKEKAKAEHRDKLGERDDTIPPEYRHLLDDNGQDKPVKPPTK
KSEDSKKPADDQDPIDALSGDLDSCPSTTETSQNTAKDKCKKAASSSKAPKNGGKAKD
SAKTTEETSKPKDD"
BASE COUNT 845 a 557 c 579 g 444 t
ORIGIN
1 gtgtgagccc ggcgcctcca acgcaacacc ccgcgccctc gccggctccc ccgccgtgcg
61 gatcggagcc agccggttgt tgccatggca ttcgccagct ggtggtacaa gacgcatgtc
121 agtgaaaaaa ccagtgaatc gccttccaaa ccaggagaaa agaaaggatc agatgagaaa
181 aaagcagcaa gcctcggcag cagtcagtcc tccagaacct atgctggtgg aacagcctcg
241 gccaccaagg tgtcagcttc ctctggtgca accagcaagt cttccagtat gaatcccaca
301 gaaaccaagg ctgtaaaaac agaacctgag aagaagtcac agtcaaccaa gctgtctgtg
361 gttcatgaga aaaaatccca agaaggaaag ccaaaagaac acacagagcc aaaaagccta
421 cccaagcagg catcagatac aggaagtaac gatgctcaca ataaaaaagc agtttccaga
481 tcagctgaac agcagccatc agagaaatca acagaaccaa agactaaacc acaagacatg
541 atttctgctg gtggagagag tgttgctggt atcactgcaa tatctggcaa gccgggtgac
601 aagaaaaaag aaaagaaatc attaacccca gctgtgccag ttgaatctaa accggataaa
661 ccatcgggaa agtcaggcat ggatgctgct ttggatgact taatagatac tttaggagga
721 cctgaagaaa ctgaagaaga aaatacaacg tatactggac cagaagtttc agatccaatg
781 agttccacct acatagagga attgggtaaa agagaagtca caattcctcc aaaatatagg
841 gaactattgg ctaaaaagga agggatcaca gggcctcctg cagactcttc gaaacccata
901 gggccagatg atgctataga cgccttgtca tctgacttca cctgtgggtc gcctacagct
961 gctggaaaga aaactgaaaa agaggaatct acagaagttt taaaagctca gtcagcaggg
1021 acagtcagaa gtgctgctcc accccaagag aagaaaagaa aggtggagaa ggatacaatg
1081 agtgatcaag cactcgaggc tctgtcggct tcactgggca cccggcaagc agaacctgag
1141 ctcgacctcc gctcaattaa ggaagtcgat gaggcaaaag ctaaagaaga aaaactagag
1201 aagtgtggtg aggatgatga aacaatccca tctgagtaca gattaaaacc agccacggat
1261 aaagatggaa aaccactatt gccagagcct gaagaaaaac ccaagcctcg gagtgaatca
1321 gaactcattg atgaactttc agaagatttt gaccggtctg aatgtaaaga gaaaccatct
1381 aagccaactg aaaagacaga agaatctaag gccgctgctc cagctcctgt gtcggaggct
1441 gtgtgtcgga cctccatgtg tagtatacag tcagcacccc ctgagccggc taccttgaag
1501 ggcacagtgc cagatgatgc tgtagaagcc ttggctgata gcctggggaa aaaggaagca
1561 gatccagaag atggaaaacc tgtgatggat aaagtcaagg agaaggccaa agaagaagac
1621 cgtgaaaagc ttggtgaaaa agaagaaaca attcctcctg attatagatt agaagaggtc
1681 aaggataaag atggaaagcc actcctgcca aaagagtcta aggaacagct tccacccatg
1741 agtgaagact tccttctgga tgctttgtct gaggacttct ctggtccaca aaatgcttca
1801 tctcttaaat ttgaagatgc taaacttgct gctgccatct ctgaagtggt ttcccaaacc
1861 ccagcttcaa cgacccaagc tggagcccca ccccgtgata cctcgcagag tgacaaagac
1921 ctcgatgatg ccttggataa actctctgac agtctaggac aaaggcagcc tgacccagat
1981 gagaacaaac caatggaaga taaagtaaag gaaaaagcta aagctgaaca tagagacaag
2041 cttggagaaa gagatgacac tatcccacct gaatacagac atctcctgga tgataatgga
2101 caggacaaac cagtgaagcc acctacaaag aaatcagagg attcaaagaa acctgcagat
2161 gaccaagacc ccattgatgc tctctcagga gatctggaca gctgtccctc cactacagaa
2221 acctcacaga acacagcaaa ggataagtgc aagaaggctg cttccagctc caaagcacct
2281 aagaatggag gtaaagcgaa ggattcagca aagacaacag aggaaacttc caagccaaaa
2341 gatgactaaa gaaatacaag ttaaggtatc tggtatctgc atgtaaaatc ttcagctggt
2401 ggatggtgac ttttgaagaa caaaa
//