LOCUS AK296826 2469 bp mRNA linear HUM 21-JAN-2009
DEFINITION Homo sapiens cDNA FLJ59311 complete cds, highly similar to
Long-chain-fatty-acid--CoA ligase 1 (EC 6.2.1.3).
ACCESSION AK296826
VERSION AK296826.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2469)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..2469
/clone="CTONG2017198"
/clone_lib="CTONG2"
/db_xref="H-InvDB:HIT000491450"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="tumor tissue"
/organism="Homo sapiens"
/tissue_type="tongue, tumor tissue"
CDS 295..2391
/codon_start=1
/note="highly similar to Long-chain-fatty-acid--CoA
ligase 1 (EC 6.2.1.3)"
/protein_id="BAH12438.1"
/transl_table=1
/translation="MQAHELFRYFRMPELVDFRQYVRTLPTNTLMGFGAFAALTTFWY
ATRPKPLKPPCDLSMQSVEVAGSGGARRSALLDSDEPLVYFYDDVTTLYEGFQRGIQV
SNNGPCLGSRKPDQPYEWLSYKQVAELSECIGSALIQKGFKTAPDQFIGIFAQNRPEW
VIIEQGCFAYSMVIVPLYDTLGNEAITYIVNKAELSLVFVDKPEKAKLLLEGVENKLI
PGLKIIVVMDAYGSELVERGQRCGVEVTSMKAMEDLGRANRRKPKPPAPEDLAVICFT
SGTTGNPKGAMVTHRNIVSDCSAFVKATEKALPLSASDTHISYLPLAHIYEQLLKCVM
LCHGAKIGFFQGDIRLLMDDLKVLQPTVFPVVPRLLNRMFDRIFGQANTTLKRWLLDF
ASKRKEAELRSGIIRNNSLWDRLIFHKVQSSLGGRVRLMVTGAAPVSATVLTFLRAAL
GCQFYEGYGQTECTAGCCLTMPGDWTAGHVGAPMPCNLIKLVDVEEMNYMAAEGEGEV
CVKGPNVFQGYLKDPAKTAEALDKDGWLHTGDIGKWLPNGTLKIIDRKKHIFKLAQGE
YIAPEKIENIYMRSEPVAQVFVHGESLQAFLIAIVVPDVETLCSWAQKRGFEGSFEEL
CRNKDVKKAILEDMVRLGKDSGLKPFEQVKGITLHPELFSIDNGLLTPTMKAKRPELR
NYFRSQIDDLYSTIKV"
BASE COUNT 657 a 571 c 649 g 592 t
ORIGIN
1 gactttgctg ctggctgccc attggcctat cctccaccct ctgctctcct gagaagtcaa
61 gtgtggattt tgtctgctag ttacctttag cccagtgggc agcagcgtgg ggctcggcat
121 gtgacttgtg ccctgagaga ccttggtagg ctgggggagc gcagctgagc cctgctaacc
181 cccgcccact tccaggctca ttgccctctt ctgtgtctgc agtggggtgt cacctagtag
241 tcagctgttg gtggcacaga tgaattcagc ttagagaact atcaacacag gacaatgcaa
301 gcccatgagc tgttccggta ttttcgaatg ccagagctgg ttgacttccg acagtacgtg
361 cgtactcttc cgaccaacac gcttatgggc ttcggagctt ttgcagcact caccaccttc
421 tggtacgcca cgagacccaa acccctgaag ccgccatgcg acctctccat gcagtcagtg
481 gaagtggcgg gtagtggtgg tgcacgaaga tccgcactac ttgacagcga cgagcccttg
541 gtgtatttct atgatgatgt cacaacatta tacgaaggtt tccagagggg aatacaggtg
601 tcaaataatg gcccttgttt aggctctcgg aaaccagacc aaccctatga atggctttca
661 tataaacagg ttgcagaatt gtcggagtgc ataggctcag cactgatcca gaagggcttc
721 aagactgccc cagatcagtt cattggcatc tttgctcaaa atagacctga gtgggtgatt
781 attgaacaag gatgctttgc ttattcgatg gtgatcgttc cactttatga tacccttgga
841 aatgaagcca tcacgtacat agtcaacaaa gctgaactct ctctggtttt tgttgacaag
901 ccagagaagg ccaaactctt attagagggt gtagaaaata agttaatacc aggccttaaa
961 atcatagttg tcatggatgc ctacggcagt gaactggtgg aacgaggcca gaggtgtggg
1021 gtggaagtca ccagcatgaa ggcgatggag gacctgggaa gagccaacag acggaagccc
1081 aagcctccag cacctgaaga tcttgcagta atttgtttca caagtggaac tacaggcaac
1141 cccaaaggag caatggtcac tcaccgaaac atagtgagcg attgttcagc ttttgtgaaa
1201 gcaacagaga aagcacttcc cttgagtgcc agtgacacac acatttcata tttaccactt
1261 gctcacattt atgaacagtt attgaagtgt gtaatgctgt gtcatggagc taaaatcgga
1321 tttttccaag gagatatcag gctgctcatg gatgacctca aggtgcttca acccactgtc
1381 ttccccgtgg ttccaagact gctgaaccgg atgtttgacc gaattttcgg acaagcaaac
1441 accacgctga agcggtggct cttggacttt gcctccaaga ggaaagaagc agagcttcgc
1501 agcggcatca tcagaaacaa cagcctgtgg gaccggctga tcttccacaa agtacagtcg
1561 agcctgggcg gaagagtccg gctgatggtg acaggagccg ccccggtgtc tgccactgtg
1621 ctgacgttcc tcagagcagc cctgggctgt cagttttatg aaggatacgg acagacagag
1681 tgcactgccg ggtgctgcct aaccatgcct ggagactgga ccgcaggcca tgttggggcc
1741 ccgatgccgt gcaatttgat aaaacttgtt gatgtggaag aaatgaatta catggctgcc
1801 gagggcgagg gcgaggtgtg tgtgaaaggg ccaaatgtat ttcagggcta cttgaaggac
1861 ccagcgaaaa cagcagaagc tttggacaaa gacggctggt tacacacagg ggacattgga
1921 aaatggttac caaatggcac cttgaaaatt atcgaccgga aaaagcacat atttaagctg
1981 gcacaaggag aatacatagc ccctgaaaag attgaaaata tctacatgcg aagtgagcct
2041 gttgctcagg tgtttgtcca cggagaaagc ctgcaggcat ttctcattgc aattgtggta
2101 ccagatgttg agacattatg ttcctgggcc caaaagagag gatttgaagg gtcgtttgag
2161 gaactgtgca gaaataagga tgtcaaaaaa gctatcctcg aagatatggt gagacttggg
2221 aaggattctg gtctgaaacc atttgaacag gtcaaaggca tcacattgca ccctgaatta
2281 ttttctatcg acaatggcct tctgactcca acaatgaagg cgaaaaggcc agagctgcgg
2341 aactatttca ggtcgcagat agatgacctc tattccacta tcaaggttta gtgtgaagaa
2401 gaaagctcag aggaaatggc acagttccac aatctcttct cctgctgatg gccttcatgt
2461 tgttaattt
//