LOCUS AK296729 1729 bp mRNA linear HUM 24-JUL-2008
DEFINITION Homo sapiens cDNA FLJ60647 complete cds, highly similar to
Keratin, type II cytoskeletal 6B.
ACCESSION AK296729
VERSION AK296729.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1729)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..1729
/clone="CTONG2005801"
/clone_lib="CTONG2"
/db_xref="H-InvDB:HIT000491353"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="tumor tissue"
/organism="Homo sapiens"
/tissue_type="tongue, tumor tissue"
CDS 110..1690
/codon_start=1
/note="highly similar to Keratin, type II cytoskeletal 6B"
/protein_id="BAG59316.1"
/transl_table=1
/translation="MASTSTTIRSHSSSRRGFSASSARLPGVSRSGFSSISVSRSRGS
GGLGGACGGAGFGSRSLYGLGGSKRISIGGGSCAISGGYGSRAGGSYGFGGAGSGFGF
GGGAGIGFGLGGGAGLAGGFGGPGFPVCPPGGIQEVTVNQSLLTPLNLQIDPAIQRVR
AEEREQIKTLNNKFASFIDKVRFLEQQNKVLDTKWTLLQEQGTKTVRQNLEPLFEQYI
NNLRRQLDSIVGERGRLDSELRNMQDLVEDLKNKYEDEINFLRALYDAELSQMQTHIS
DTSVVLSMDNNRNLDLDSIIAEVKAQYEEIAQRSRAEAESWYQTKYEELQVTAGRHGD
DLRNTKQEIAEINRMIQRLRSEIDHVKKQCANLQAAIADAEQRGEMALKDAKNKLEGL
EDALQKAKQDLARLLKEYQELMNVKLALDVEIATYRKLLEGEECRLNGEGVGQVNISV
VQSTVSSGYGGASGVGSGLGLGGGSSYSYGSGLGVGGGFSSSSGRATGGGLSSVGGGS
STIKYTTTSSSSRKSYKH"
BASE COUNT 370 a 488 c 548 g 323 t
ORIGIN
1 atgctctcca tatataagct gctactggag tccgattcct cgtcctgctt ctcctccctc
61 tcgcctccag cctctcacac tctcctaagc cctctcatct cctggaacca tggccagcac
121 atccaccacc atcaggagcc acagcagcag ccgccggggt ttcagtgcca gctcggccag
181 gctccctggg gtcagccgct ctggcttcag cagcatctcc gtgtcccgct ccaggggcag
241 tggtggcctg ggtggcgcat gtggaggagc tggctttggc agccgcagtc tgtatggcct
301 ggggggctcc aagaggatct ccattggagg gggcagctgt gccatcagtg gcggctatgg
361 cagcagagcc ggaggcagct atggctttgg tggcgccggg agtggatttg gtttcggtgg
421 tggagccggc attggctttg gtctgggtgg tggagccggc cttgctggtg gctttggggg
481 ccctggcttc cctgtgtgcc cccctggagg catccaagag gtcactgtca accagagtct
541 cctgactccc ctcaacctgc aaattgaccc cgccatccag cgggtgcggg ccgaggagcg
601 tgagcagatc aagaccctca acaacaagtt tgcctccttc atcgacaagg tgcggttcct
661 agagcagcag aacaaggttc tggacaccaa gtggaccctg ctgcaggagc agggcaccaa
721 gactgtgagg cagaacctgg agccgttgtt cgagcagtac atcaacaacc tcaggaggca
781 gctggacagc atcgtggggg aacggggtcg tctggactcg gagctgagaa acatgcagga
841 cctggtggag gacctcaaga acaaatatga ggatgagatc aacttcctga gagccttgta
901 tgatgcagag ctgtcccaga tgcagaccca catctcagac acatccgtgg tgctatccat
961 ggacaacaac cgcaacctgg acctggacag catcatcgct gaggtcaagg cccaatatga
1021 ggagattgct cagaggagca gggctgaggc tgagtcctgg taccagacaa agtacgagga
1081 gctgcaggtc acagcaggca gacatgggga cgacctgcgc aacaccaagc aggagattgc
1141 tgagatcaac cgcatgatcc agaggctgag atctgagatc gaccacgtca agaagcagtg
1201 tgccaaccta caggctgcca ttgctgatgc tgagcagcgt ggggagatgg ccctcaagga
1261 tgctaagaac aagctggaag ggctggagga tgccctgcag aaggccaagc aggacctggc
1321 ccggctgctg aaggagtacc aggagctgat gaacgtcaag ctggccctgg atgtggagat
1381 cgccacctac cgcaagctgc tggagggcga ggagtgcagg ctgaatggcg aaggcgttgg
1441 acaagtcaac atctctgtag tgcagtccac cgtctccagt ggctatggcg gtgccagcgg
1501 tgtcggcagt ggcttaggcc tgggtggagg aagcagctac tcctatggca gtggtcttgg
1561 cgttggaggc ggctttagtt ccagcagcgg cagagccact gggggtggcc tcagctctgt
1621 tggaggcggc agttccacca tcaagtacac caccacctcc tcctccagca ggaagagcta
1681 caagcactga agtgctgccg ccagctctca gtcccacagc tctcaggcc
//