LOCUS AK296530 2038 bp mRNA linear HUM 24-JUL-2008
DEFINITION Homo sapiens cDNA FLJ59561 complete cds, highly similar to
Galactocerebrosidase precursor (EC 3.2.1.46).
ACCESSION AK296530
VERSION AK296530.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2038)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..2038
/clone="BRTHA3024899"
/clone_lib="BRTHA3"
/db_xref="H-InvDB:HIT000491154"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/organism="Homo sapiens"
/tissue_type="thalamus"
CDS 47..1987
/codon_start=1
/note="highly similar to Galactocerebrosidase precursor
(EC 3.2.1.46)"
/protein_id="BAG59160.1"
/transl_table=1
/translation="MTAAAGSAGRAAVPLLLCALLAPGGAYVLDDSDGLGREFDGIGA
VSGGGPNFGASLHILKVEIGGDGQTTDGTEPSHMHYALDENYFRGYEWWLMKEAKKRN
PNITLIGLPWSFPGWLGKGFDWPYVNLQLTAYYVVTWIVGAKCYHDLDIDYIGIWNER
SYNANYIKILRKMLNYQGLQRVKIIASDNLWESISASMLLDAELFKVVDVIGAHYPGT
HSAKDAKLTGKKLWSSEDFSTLNSDMGAGCWGRILNQNYINGYMTSTIAWNLVASYYE
QLPYGRCGLMTAQEPWSGHYVVESPVWVSAHTTQFTQPGWYYLKTVGHLEKGGSYVAL
TDGLGNLTIIIETMSHKHSKCIRPFLPYFNVSQQFATFVLKGSFSEIPELQVWYTKLG
KTSERFLFKQLDSLWLLDSDGSFTLSLHEDELFTLTTLTTGRKGSYPLPPKSQPFPST
YKDDFNVDYPFFSEAPNFADQTGVFEYFTNIEDPGEHHFTLRQVLNQRPITWAADASN
TISIIGDYNWTNLTTKCDVYIETPDTGGVFIAGRVNKGGILIRSARGIFFWIFANGSY
RVTGDLAGWIIYALGRVEVTAKKWYTLTLTIKGHFASGMLNDKSLWTDIPVNFPKNGW
AAIGTHSFEFAQFDNFLVEATR"
BASE COUNT 535 a 438 c 497 g 568 t
ORIGIN
1 ggctgagtgg ctactctcgg cttcctggca acgccgagcg aaagctatga ctgcggccgc
61 gggttcggcg ggccgcgccg cggtgccctt gctgctgtgt gcgctgctgg cgcccggcgg
121 cgcgtacgtg ctcgacgact ccgacgggct gggccgggag ttcgacggca tcggcgcggt
181 cagcggcggc gggccgaatt ttggtgcctc tttgcatatt ttaaaagtgg aaataggtgg
241 tgatgggcag acaacagacg gcactgagcc ctcccacatg cattatgcac tagatgagaa
301 ttatttccga ggatacgagt ggtggttgat gaaagaagct aagaagagga atcccaatat
361 tacactcatt gggttgccat ggtcattccc tggatggctg ggaaaaggtt tcgactggcc
421 ttatgtcaat cttcagctga ctgcctatta tgtcgtgacc tggattgtgg gcgccaagtg
481 ttaccatgat ttggacattg attatattgg aatttggaat gagaggtcat ataatgccaa
541 ttatattaag atattaagaa aaatgctgaa ttatcaaggt ctccagcgag tgaaaatcat
601 agcaagtgat aatctctggg agtccatctc tgcatccatg ctccttgatg ccgaactctt
661 caaggtggtt gatgttatag gggctcatta tcctggaacc cattcagcaa aagatgcaaa
721 gttgactggg aagaagcttt ggtcttctga agactttagc actttaaata gtgacatggg
781 tgcaggctgc tggggtcgca ttttaaatca gaattatatc aatggctata tgacttccac
841 aatcgcatgg aatttagtgg ctagttacta tgaacagttg ccttatggga gatgcgggtt
901 gatgacggcc caggagccat ggagtgggca ctacgtggta gaatctcctg tctgggtatc
961 agctcatacc actcagttta ctcaacctgg ctggtattac ctgaagacag ttggccattt
1021 agagaaagga ggaagctacg tagctctgac tgatggctta gggaacctca ccatcatcat
1081 tgaaaccatg agtcataaac attctaagtg catacggcca tttcttcctt atttcaatgt
1141 gtcacaacaa tttgccacct ttgttcttaa gggatctttt agtgaaatac cagagctaca
1201 ggtatggtat accaaacttg gaaaaacatc cgaaagattt ctttttaagc agctggattc
1261 tctatggctc cttgacagtg atggcagttt cacactgagc ctgcatgaag atgagctgtt
1321 cacactcacc actctcacca ctggtcgcaa aggcagctac ccgcttcctc caaaatccca
1381 gcccttccca agtacctata aggatgattt caatgttgat tacccatttt ttagtgaagc
1441 tccaaacttt gctgatcaaa ctggtgtatt tgaatatttt acaaatattg aagaccctgg
1501 cgagcatcac ttcacgctac gccaagttct caaccagaga cccattacgt gggctgccga
1561 tgcatccaac acaatcagta ttataggaga ctacaactgg accaatctga ctacaaagtg
1621 tgatgtttac atagagaccc ctgacacagg aggtgtgttc attgcaggaa gagtaaataa
1681 aggtggtatt ttgattagaa gtgccagagg aattttcttc tggatttttg caaatggatc
1741 ttacagggtt acaggtgatt tagctggatg gattatatat gctttaggac gtgttgaagt
1801 tacagcaaaa aaatggtata cactcacgtt aactattaag ggtcatttcg cctctggcat
1861 gctgaatgac aagtctctgt ggacagacat ccctgtgaat tttccaaaga atggctgggc
1921 tgcaattgga actcactcct ttgaatttgc acagtttgac aactttcttg tggaagccac
1981 acgctaatac ttaacagggc atcatagaat actctggatt ttcttccctt ctttttgg
//