LOCUS AK296510 3892 bp mRNA linear HUM 24-JUL-2008
DEFINITION Homo sapiens cDNA FLJ55300 partial cds, highly similar to DNA
topoisomerase 2-beta (EC 5.99.1.3).
ACCESSION AK296510
VERSION AK296510.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3892)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..3892
/clone="BRTHA3022466"
/clone_lib="BRTHA3"
/db_xref="H-InvDB:HIT000491134"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/organism="Homo sapiens"
/tissue_type="thalamus"
CDS 359..>3892
/codon_start=1
/note="highly similar to DNA topoisomerase 2-beta (EC
5.99.1.3)"
/protein_id="BAG59142.1"
/transl_table=1
/translation="MWVYDEDVGMNCREVTFVPGLYKIFDEILVNAADNKQRDKNMTC
IKVSIDPESNIISIWNNGKGIPVVEHKVEKVYVPALIFGQLLTSSNYDDDEKKVTGGR
NGYGAKLCNIFSTKFTVETACKEYKHSFKQTWMNNMMKTSEAKIKHFDGEDYTCITFQ
PDLSKFKMEKLDKDIVALMTRRAYDLAGSCRGVKVMFNGKKLPVNGFRSYVGLYVKDK
LDETGVALKVIHELANERWDVCLTLSEKGFQQISFVNSIATTKGGRHVDYVVDQVVGK
LIEVVKKKNKAGVSVKPFQVKNHIWVFINCLIENPTFDSQTKENMTLQPKSFGSKCQL
SEKFFKAASNCGIVESILNWVKFKAQTQLNKKCSSVKYSKIKGIPKLDDANDAGGKHS
LECTLILTEGDSAKSLAVSGLGVIGRDRYGVFPLRGKILNVREASHKQIMENAEINNI
IKIVGLRYKKSYDDAESLKTLRYGKIMIMTDQDQDGSHIKGLLINFIHHNWPSLLKHG
FLEEFITPIVKASKNKQELSFYSIPEFDEWKKHIENQKAWKIKYYKGLGTSTAKEAKE
YFADMERHRILFRYAGPEDDAAITLAFSKKKIDDRKEWLTNFMEDRRQRRLHGLPEQF
LYGTATKHLTYNDFINKELILFSNSDNERSIPSLVDGFKPGQRKVLFTCFKRNDKREV
KVAQLAGSVAEMSAYHHGEQALMMTIVNLAQNFVGSNNINLLQPIGQFGTRLHGGKDA
ASPRYIFTMLSTLARLLFPAVDDNLLKFLYDDNQRVEPEWYIPIIPMVLINGAEGIGT
GWACKLPNYDAREIVNNVRRMLDGLDPHPMLPNYKNFKGTIQELGQNQYAVSGEIFVV
DRNTVEITELPVRTWTQVYKEQVLEPMLNGTDKTPALISDYKEYHTDTTVKFVVKMTE
EKLAQAEAAGLHKVFKLQTTLTCNSMVLFDHMGCLKKYETVQDILKEFFDLRLSYYGL
RKEWLVGMLGAESTKLNNQARFILEKIQGKITIENRSKKDLIQMLVQRGYESDPVKAW
KEAQEKAAEEDETQNQHDDSSSDSGTPSGPDFNYILNMSLWSLTKEKVEELIKQRDAK
GREVNDLKRKSPSDLWKEDLAAFVEELDKVESQEREDVLAGMSGKAIKGKVGKPKVKK
LQLEETMPSPYGRRIIPEITAMKADASKKLLK"
BASE COUNT 1302 a 618 c 891 g 1081 t
ORIGIN
1 gtgtgtgcgg tgttatgccg gacaagaggg aggtgaccgt ggcggcggcg gcggcggctc
61 tgtttattgt ccctctcggt gtgtgtgtgt gaggaaatcg gggctgcagc gaggctaagg
121 ctgcctttga agcagcggcg gcgaccggga cgactactct ggcaacgggg cactgacctg
181 ggtgactctt tttgatcaga acaatgctgc aaaaaaagaa gagtcagaaa ctgccaacaa
241 aaatgattct tcaaagaagt tgtctgttga gagagtgtat cagaagaaga cacaacttga
301 acacattctt cttcgtcctg atacatatat tgggtcagtg gagccattga cgcagttcat
361 gtgggtgtat gatgaagatg taggaatgaa ttgcagggag gttacctttg tgccaggttt
421 atacaagatc tttgatgaaa ttttggttaa tgctgctgac aataaacaga gggataagaa
481 catgacttgt attaaagttt ctattgatcc tgaatctaac attataagca tttggaataa
541 tgggaaaggc attccagtag tagaacacaa ggtagagaaa gtttatgttc ctgctttaat
601 ttttggacag cttttaacat ccagtaacta tgatgatgat gagaaaaaag ttacaggtgg
661 tcgtaatggt tatggtgcaa aactttgtaa tattttcagt acaaagttta cagtagaaac
721 agcttgcaaa gaatacaaac acagttttaa gcagacatgg atgaataata tgatgaagac
781 ttctgaagcc aaaattaaac attttgatgg tgaagattac acatgcataa cattccaacc
841 agatctgtcc aaatttaaga tggaaaaact tgacaaggat attgtggccc tcatgactag
901 aagggcatat gatttggctg gttcgtgtag aggggtcaag gtcatgttta atggaaagaa
961 attgcctgta aatggatttc gcagttatgt aggtctttat gtgaaagaca aattggatga
1021 aactggggtg gccctgaaag ttattcatga gcttgcaaat gaaagatggg atgtttgtct
1081 cacattgagt gaaaaaggat tccagcaaat cagctttgta aatagtattg caactacaaa
1141 aggtggacgg cacgtggatt atgtggtaga tcaagttgtt ggtaaactga ttgaagtagt
1201 taagaaaaag aacaaagctg gtgtatcagt gaaaccattt caagtaaaaa accatatatg
1261 ggtttttatt aattgcctta ttgaaaatcc aacttttgat tctcagacta aggaaaacat
1321 gactctgcag cccaaaagtt ttgggtctaa atgccagctg tcagaaaaat tttttaaagc
1381 agcctctaat tgtggcattg tagaaagtat cctgaactgg gtgaaattta aggctcagac
1441 tcagctgaat aagaagtgtt catcagtaaa atacagtaaa atcaaaggta ttcccaaact
1501 ggatgatgct aatgatgctg gtggtaaaca ttccctggag tgtacactga tattaacaga
1561 gggagactct gccaaatcac tggctgtgtc tggattaggt gtgattggac gagacagata
1621 cggagttttt ccactcaggg gcaaaattct taatgtacgg gaagcttctc ataaacagat
1681 catggaaaat gctgaaataa ataatattat taaaatagtt ggtctacgat ataagaaaag
1741 ttacgatgat gcagaatctc tgaaaacctt acgctatgga aagattatga ttatgaccga
1801 tcaggatcaa gatggttctc acataaaagg cctgcttatt aatttcatcc atcacaattg
1861 gccatcactt ttgaagcatg gttttcttga agagttcatt actcctattg taaaggcaag
1921 caaaaataag caggaacttt ccttctacag tattcctgaa tttgacgaat ggaaaaaaca
1981 tatagaaaac cagaaagcct ggaaaataaa gtactataaa ggattgggta ctagtacagc
2041 taaagaagca aaggaatatt ttgctgatat ggaaaggcat cgcatcttgt ttagatatgc
2101 tggtcctgaa gatgatgctg ccattacctt ggcatttagt aagaagaaga ttgatgacag
2161 aaaagaatgg ttaacaaatt ttatggaaga ccggagacag cgtaggctac atggcttacc
2221 agagcaattt ttatatggta ctgcaacaaa gcatttgact tataatgatt tcatcaacaa
2281 ggaattgatt ctcttctcaa actcagacaa tgaaagatct ataccatctc ttgttgatgg
2341 ctttaaacct ggccagcgga aagttttatt tacctgtttc aagaggaatg ataaacgtga
2401 agtaaaagtt gcccagttgg ctggctctgt tgctgagatg tcggcttatc atcatggaga
2461 acaagcattg atgatgacta ttgtgaattt ggctcagaac tttgtgggaa gtaacaacat
2521 taacttgctt cagcctattg gtcagtttgg aactcggctt catggtggca aagatgctgc
2581 aagccctcgt tatattttca caatgttaag cactttagca aggctacttt ttcctgctgt
2641 ggatgacaac ctccttaagt tcctttatga tgataatcaa cgtgtagagc ctgagtggta
2701 tattcctata attcccatgg ttttaataaa tggtgctgag ggcattggta ctggatgggc
2761 ttgtaaacta cccaactatg atgctaggga aattgtgaac aatgtcagac gaatgctaga
2821 tggcctggat cctcatccca tgcttccaaa ctacaaaaac tttaaaggca cgattcaaga
2881 acttggtcaa aaccagtatg cagtcagtgg tgaaatattt gtagtggaca gaaacacagt
2941 agaaattaca gagcttccag ttagaacttg gacacaggta tataaagaac aggttttaga
3001 acctatgcta aatggaacag ataaaacacc agcattaatt tctgattata aagaatatca
3061 tactgacaca actgtgaaat ttgtggtgaa aatgactgaa gagaaactag cacaagcaga
3121 agctgctgga ctgcataaag tttttaaact tcaaactact cttacttgta attccatggt
3181 actttttgat catatgggat gtctgaagaa atatgaaact gtgcaagaca ttctgaaaga
3241 attctttgat ttacgattaa gttattacgg tttacgtaag gagtggcttg tgggaatgtt
3301 gggagcagaa tctacaaagc ttaacaatca agcccgtttc attttagaga agatacaagg
3361 gaaaattact atagagaata ggtcaaagaa agatttgatt caaatgttag tccagagagg
3421 ttatgaatct gacccagtga aagcctggaa agaagcacaa gaaaaggcag cagaagagga
3481 tgaaacacaa aaccagcatg atgatagttc ctccgattca ggaactcctt caggcccaga
3541 ttttaattat attttaaata tgtctctgtg gtctcttact aaagaaaaag ttgaagaact
3601 gattaaacag agagatgcaa aagggcgaga ggtcaatgat cttaaaagaa aatctccttc
3661 agatctttgg aaagaggatt tagcggcatt tgttgaagaa ctggataaag tggaatctca
3721 agaacgagaa gatgttctgg ctggaatgtc tggaaaagca attaaaggta aagttggcaa
3781 acctaaggtg aagaaactcc agttggaaga gacaatgccc tcaccttatg gcagaagaat
3841 aattcctgaa attacagcta tgaaggcaga tgccagcaaa aagttgctga ag
//