LOCUS AK296396 3861 bp mRNA linear HUM 21-JAN-2009
DEFINITION Homo sapiens cDNA FLJ60617 complete cds, highly similar to
Mitogen-activated protein kinase kinase kinase kinase 4 (EC
2.7.11.1).
ACCESSION AK296396
VERSION AK296396.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3861)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..3861
/clone="BRTHA3011415"
/clone_lib="BRTHA3"
/db_xref="H-InvDB:HIT000491020"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/organism="Homo sapiens"
/tissue_type="thalamus"
CDS 75..3782
/codon_start=1
/note="highly similar to Mitogen-activated protein kinase
kinase kinase kinase 4 (EC 2.7.11.1)"
/protein_id="BAH12341.1"
/transl_table=1
/translation="MANDSPAKSLVDIDLSSLRDPAGIFELVEVVGNGTYGQVYKGRH
VKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLW
LVMEFCGAGSITDLVKNTKGNTLKEDWIAYISREILRGLAHLHIHHVIHRDIKGQNVL
LTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWS
CGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYMQ
RPSTEQLLKHPFIRDQPNERQVRIQLKDHIDRTRKKRGEKDETEYEYSGSEEEEEEVP
EQEGEPSSIVNVPGESTLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLL
AERQKRIEQQKEQRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRKEEEE
RRRAEEEKRRVEREQEYIRRQLEEEQRHLEVLQQQLLQEQAMLLHDHRRPHPQHSQQP
PPPQQERSKPSFHAPEPKAHYEPADRAREVQWSHLASLKNNVSPVSRSHSFSDPSPKF
AHHHLRSQDPCPPSRSEVLSQSSDSKSEAPDPTQKAWSRSDSDEVPPRVPVRTTSRSP
VLSRRDSPLQGSGQQNSQAGQRNSTSSIEPRLLWERVEKLVPRPGSGSSSGSSNSGSQ
PGSHPGSQSGSGERFRVRSSSKSEGSPSQRLENAVKKPEDKKEVFRPLKPAGEVDLTA
LAKELRAVEDVRPPHKVTDYSSSSEESGTTDEEDDDVEQEGADESTSGPEDTRAASSL
NLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQTQSASSTLQKHKSSSSFTPFI
DPRLLQISPSSGTTVTSMVGFSCDGMRPEAIRQDPTRKGSVVNVNPTNTRPQSDTPEI
RKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLINRRRFQQMDVLEGL
NVLVTISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERI
KFLVIALKSSVEVYAWAPKPYHKFMAFKSFGELVHKPLLVDLTVEEGQRLKVIYGSCA
GFHAVDVDSGSVYDIYLPTHIQCSIKPHAIIILPNTDGMELLVCYEDEGVYVNTYGRI
TKDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCE
RNDKVFFASVRSGGSSQVYFMTLGRTSLLSW"
BASE COUNT 1117 a 853 c 1051 g 840 t
ORIGIN
1 ctggtctgcg gctgagatac acagagcgac agagacattt attgttattt gttttttggt
61 ggcaaaaagg gaaaatggcg aacgactccc ctgcaaaaag tctggtggac atcgacctct
121 cctccctgcg ggatcctgct gggatttttg agctggtgga agtggttgga aatggcacct
181 atggacaagt ctataagggt cgacatgtta aaacgggtca gttggcagcc atcaaagtta
241 tggatgtcac tgaggatgaa gaggaagaaa tcaaactgga gataaatatg ctaaagaaat
301 actctcatca cagaaacatt gcaacatatt atggtgcttt catcaaaaag agccctccag
361 gacatgatga ccaactctgg cttgttatgg agttctgtgg ggctgggtcc attacagacc
421 ttgtgaagaa caccaaaggg aacacactca aagaagactg gatcgcttac atctccagag
481 aaatcctgag gggactggca catcttcaca ttcatcatgt gattcaccgg gatatcaagg
541 gccagaatgt gttgctgact gagaatgcag aggtgaaact tgttgacttt ggtgtgagtg
601 ctcagctgga caggactgtg gggcggagaa atacgttcat aggcactccc tactggatgg
661 ctcctgaggt catcgcctgt gatgagaacc cagatgccac ctatgattac agaagtgatc
721 tttggtcttg tggcattaca gccattgaga tggcagaagg tgctccccct ctctgtgaca
781 tgcatccaat gagagcactg tttctcattc ccagaaaccc tcctccccgg ctgaagtcaa
841 aaaaatggtc gaagaagttt tttagtttta tagaagggtg cctggtgaag aattacatgc
901 agcggccctc tacagagcag cttttgaaac atccttttat aagggatcag ccaaatgaaa
961 ggcaagttag aatccagctt aaggatcata tagatcgtac caggaagaag agaggcgaga
1021 aagatgaaac tgagtatgag tacagtggga gtgaggaaga agaggaggaa gtgcctgaac
1081 aggaaggaga gccaagttcc attgtgaacg tgcctggtga gtctactctt cgccgagatt
1141 tcctgagact gcagcaggag aacaaggaac gttccgaggc tcttcggaga caacagttac
1201 tacaggagca acagctccgg gagcaggaag aatataaaag gcaactgctg gcagagagac
1261 agaagcggat tgagcagcag aaagaacaga ggcgacggct agaagagcaa caaaggagag
1321 agcgggaagc tagaaggcag caggaacgtg aacagcgaag gagagaacaa gaagaaaaga
1381 ggcgtctaga ggagttggag agaaggcgca aagaagaaga ggagaggaga cgggcagaag
1441 aagaaaagag gagagttgaa agagaacagg agtatatcag gcgacagcta gaagaggagc
1501 agcggcactt ggaagtcctt cagcagcagc tgctccagga gcaggccatg ttactgcatg
1561 accataggag gccgcacccg cagcactcgc agcagccgcc accaccgcag caggaaagga
1621 gcaagccaag cttccatgct cccgagccca aagcccacta cgagcctgct gaccgagcgc
1681 gagaggtaca gtggtcccac ctggcatctc tcaagaacaa tgtttcccct gtctcgcgat
1741 cccattcctt cagtgaccct tctcccaaat ttgcacacca ccatcttcgt tctcaggacc
1801 catgtccacc ttcccgcagt gaggtgctca gtcagagctc tgactctaag tcagaggcgc
1861 ctgaccctac ccaaaaggct tggtctagat cagacagtga cgaggtgcct ccaagggttc
1921 ctgtgagaac aacatctcgc tcccctgttc tgtcccgtcg agattcccca ctgcagggca
1981 gtgggcagca gaatagccag gcaggacaga gaaactccac cagcagtatt gagcccaggc
2041 ttctgtggga gagagtggag aagctggtgc ccagacctgg cagtggcagc tcctcagggt
2101 ccagcaactc aggatcccag cccgggtctc accctgggtc tcagagtggc tccggggaac
2161 gcttcagagt gagatcatca tccaagtctg aaggctctcc atctcagcgc ctggaaaatg
2221 cagtgaaaaa acctgaagat aaaaaggaag ttttcagacc cctcaagcct gctggcgaag
2281 tggatctgac cgcactggcc aaagagcttc gagcagtgga agatgtacgg ccacctcaca
2341 aagtaacgga ctactcctca tccagtgagg agtcggggac gacggatgag gaggacgacg
2401 atgtggagca ggaaggggct gacgagtcca cctcaggacc agaggacacc agagcagcgt
2461 catctctgaa tttgagcaat ggtgaaacgg aatctgtgaa aaccatgatt gtccatgatg
2521 atgtagaaag tgagccggcc atgaccccat ccaaggaggg cactctaatc gtccgccaga
2581 ctcagtccgc tagtagcaca ctccagaaac acaaatcttc ctcctccttt acacctttta
2641 tagaccccag attactacag atttctccat ctagcggaac aacagtgaca tctatggtgg
2701 gattttcctg tgatgggatg agaccagaag ccataaggca agatcctacc cggaaaggct
2761 cagtggtcaa tgtgaatcct accaacacta ggccacagag tgacaccccg gagattcgta
2821 aatacaagaa gaggtttaac tctgagattc tgtgtgctgc cttatgggga gtgaatttgc
2881 tagtgggtac agagagtggc ctgatgctgc tggacagaag tggccaaggg aaggtctatc
2941 ctcttatcaa ccgaagacga tttcaacaaa tggacgtact tgagggcttg aatgtcttgg
3001 tgacaatatc tggcaaaaag gataagttac gtgtctacta tttgtcctgg ttaagaaata
3061 aaatacttca caatgatcca gaagttgaga agaagcaggg atggacaacc gtaggggatt
3121 tggaaggatg tgtacattat aaagttgtaa aatatgaaag aatcaaattt ctggtgattg
3181 ctttgaagag ttctgtggaa gtctatgcgt gggcaccaaa gccatatcac aaatttatgg
3241 cctttaagtc atttggagaa ttggtacata agccattact ggtggatctc actgttgagg
3301 aaggccagag gttgaaagtg atctatggat cctgtgctgg attccatgct gttgatgtgg
3361 attcaggatc agtctatgac atttatctac caacacatat ccagtgtagc atcaaacccc
3421 atgcaatcat catcctcccc aatacagatg gaatggagct tctggtgtgc tatgaagatg
3481 agggggttta tgtaaacaca tatggaagga tcaccaagga tgtagttcta cagtggggag
3541 agatgcctac atcagtagca tatattcgat ccaatcagac aatgggctgg ggagagaagg
3601 ccatagagat ccgatctgtg gaaactggtc acttggatgg tgtgttcatg cacaaaaggg
3661 ctcaaagact aaaattcttg tgtgaacgca atgacaaggt gttctttgcc tctgttcggt
3721 ctggtggcag cagtcaggtt tatttcatga ccttaggcag gacttctctt ctgagctggt
3781 agaagcagtg tgatccaggg attactggcc tccagagtct tcaagatcct gagaacttgg
3841 aattccttgt aactggagct c
//