LOCUS AK296340 2929 bp mRNA linear HUM 21-JAN-2009
DEFINITION Homo sapiens cDNA FLJ58368 complete cds, highly similar to Rap
guanine nucleotide exchange factor 4.
ACCESSION AK296340
VERSION AK296340.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2929)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..2929
/clone="BRTHA3004269"
/clone_lib="BRTHA3"
/db_xref="H-InvDB:HIT000490964"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/organism="Homo sapiens"
/tissue_type="thalamus"
CDS 272..2794
/codon_start=1
/note="highly similar to Rap guanine nucleotide exchange
factor 4"
/protein_id="BAH12322.1"
/transl_table=1
/translation="MAGLLAPPYGVMETGSNNDRIPDKENVPSEKILRAGKILRNAIL
SRAPHMIRDRKYHLKTYRQCCVGTELVDWMMQQTPCVHSRTQAVGMWQVLLEDGVLNH
VDQEHHFQDKYLFYRFLDDEHEDAPLPTEEEKKECDEELQDTMLLLSQMGPDAHMRMI
LRKPPGQRTVDDLEIIYEELLHIKALSHLSTTVKRELAGVLIFESHAKGGTVLFNQGE
EGTSWYIILKGSVNVVIYGKGVVCTLHEGDDFGKLALVNDAPRAASIVLREDNCHFLR
VDKEDFNRILRDVEANTVRLKEHDQDVLVLEKVPAGNRASNQGNSQPQQKYTVMSGTP
EKILEHFLETIRLEATLNEATDSVLNDFIMMHCVFMPNTQLCPALVAHYHAQPSQGTE
QEKMDYALNNKRRVIRLVLQWAAMYGDLLQEDDVSMAFLEEFYVSVSDDARMIAALKE
QLPELEKIVKQISEDAKAPQKKHKVLLQQFNTGDERAQKRQPIRGSDEVLFKVYCMDH
TYTTIRVPVATSVKEVISAVADKLGSGEGLIIVKMSSGGEKVVLKPNDVSVFTTLTIN
GRLFACPREQFDSLTPLPEQEGPTVGTVGTFELMSSKDLAYQMTIYDWELFNCVHELE
LIYHTFGRHNFKKTTANLDLFLRRFNEIQFWVVTEICLCSQLSKRVQLLKKFIKIAAH
CKEYKNLNSFFAIVMGLSNVAVSRLALTWEKLPSKFKKFYAEFESLMDPSRNHRAYRL
TVAKLEPPLIPFMPLLIKDMTFTHEGNKTFIDNLVNFEKMRMIANTARTVRYYRSQPF
NPDAAQANKNHQDVRSYVRQLNVIDNQRTLSQMSHRLEPRRP"
BASE COUNT 882 a 644 c 696 g 707 t
ORIGIN
1 agattaccaa tgataaagcc ggggctacgg ctaaagaaag gtaggatgca tacgcagaaa
61 cattagtgct aatatgctgc agaaaagcca agataaatca gtccagctct catacatcac
121 taagggagaa gctgattgca acttgaccat gtgcctgagt agtcccatca gtgagaagag
181 gagaacatgg atttaaaaaa aactagaatg tacccctagg gctcctgccg tggtggacct
241 gggagaagtg tctggtaaat atcgacagta tatggcagga cttctggctc ctccttatgg
301 tgttatggaa acgggctcta acaatgacag gattcctgac aaggagaacg tcccttcaga
361 gaagatcctc agagctggaa aaattttacg aaatgccatt ctctctcgag cacctcacat
421 gataagagat agaaaatacc acctaaagac atacagacaa tgctgtgtgg gaactgaact
481 ggtggactgg atgatgcagc agacaccatg tgttcactcc cggactcaag ctgttggcat
541 gtggcaagtc ctgttagaag atggtgttct caaccacgtg gaccaggagc accatttcca
601 agacaaatat ttattctatc gatttctgga tgatgagcac gaggatgccc ctttgcctac
661 tgaggaggag aagaaggagt gtgatgagga gctccaggac accatgctgc tgctgtcaca
721 gatgggcccc gacgcccaca tgaggatgat ccttcgcaaa ccacctggcc agaggactgt
781 ggatgaccta gagattatct atgaggagct tcttcatatt aaagccttat cccatctttc
841 taccacagtg aaacgagagt tagcaggtgt tctcattttt gagtctcacg ccaaaggagg
901 gactgtgttg tttaaccagg gggaagaagg tacctcctgg tacattattc taaaaggatc
961 agtgaatgta gtcatttacg gcaagggtgt ggtctgcacc ctgcatgaag gagatgactt
1021 cggcaagtta gcactagtga atgatgcccc acgagctgcc tctatcgtct tacgagaaga
1081 taactgccat ttcttaagag tagacaagga ggatttcaac cggatcctaa gggacgtgga
1141 ggcgaataca gtcagactta aagaacatga ccaagatgtc ttggtgctgg agaaggtccc
1201 agcagggaac agagcttcta atcaaggaaa ctcacagcct cagcaaaagt atactgtgat
1261 gtcaggaaca cctgaaaaaa ttttagagca ttttctagaa acaatacgcc ttgaggcaac
1321 tttaaatgaa gcaacagatt ctgttttaaa tgactttatt atgatgcact gtgtttttat
1381 gccaaatacc cagctttgcc cggcactggt ggcccactac cacgcacagc cttcacaagg
1441 tacagaacag gagaaaatgg attatgccct caacaataag aggcgagtca tccgcctggt
1501 tctacagtgg gctgccatgt atggagacct cctgcaagag gatgacgtgt ctatggcctt
1561 cctggaggag ttttatgtat ctgtatcaga tgatgcccgg atgattgctg ccctcaagga
1621 gcaactgcca gagttggaga agattgtcaa gcaaatctca gaagatgcaa aggcaccaca
1681 aaagaagcac aaggttcttt tgcaacagtt caatacgggc gatgagagag cccagaagcg
1741 ccagcctatc cgcggctctg atgaagttct gtttaaggtc tattgcatgg accacaccta
1801 cacaaccatt cgggtgccag tggccacttc ggtgaaggaa gtcatcagtg cagttgccga
1861 caagctgggc tccggggagg gcctgatcat agtcaagatg agttccggag gagaaaaggt
1921 ggtgctcaaa cctaatgatg tttcagtatt tacgacgctc accattaatg gacgcctgtt
1981 tgcttgcccg cgagagcaat tcgattcact gactccctta ccagaacagg aaggcccaac
2041 tgttggaaca gtgggaactt ttgaactgat gagctccaaa gatttagcat accagatgac
2101 aatttatgat tgggaactct tcaactgcgt gcatgagctg gagctaatct atcacacatt
2161 tggaaggcat aattttaaaa agaccacagc aaacttggat ttgttcctga ggagatttaa
2221 tgaaattcag ttttgggtcg tcactgagat ctgcctttgt tctcagctca gcaagcgtgt
2281 tcagctatta aaaaaattta ttaagatagc agcccactgt aaggagtata aaaatctgaa
2341 ttcctttttt gccatcgtca tgggactaag taacgttgct gtgagccgct tggcactaac
2401 gtgggagaaa ctgccaagca agttcaagaa gttctatgcg gagtttgaaa gtttaatgga
2461 cccttcaagg aaccacaggg cctacaggct gacagtagct aagctggaac ctcctctcat
2521 ccccttcatg cctttgctca ttaaagatat gacatttact catgagggga acaagacgtt
2581 cattgacaat ctagtaaact ttgaaaaaat gcgcatgatt gcaaatacgg ccagaacagt
2641 gagatactac aggagccaac ccttcaatcc tgatgcagct caagctaata agaaccatca
2701 ggatgtccgg agttatgtac ggcaattaaa tgtgattgac aaccagagaa ctttatcaca
2761 gatgtcacac agattagagc ctcgtcgacc atagacattt caaatgccca aagcaacagt
2821 ttgtctccag tccacaatct ttcaaaaatg ccatttatgc tactactgac tgtattgcca
2881 ctagagaatt ctacaaaaca agcaaaaaca catcctgaga cacctcagg
//