LOCUS AK295723 2924 bp mRNA linear HUM 24-JUL-2008
DEFINITION Homo sapiens cDNA FLJ61276 complete cds, highly similar to
Probable E3 ubiquitin-protein ligase HECTD2.
ACCESSION AK295723
VERSION AK295723.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2924)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..2924
/clone="BRHIP3018689"
/clone_lib="BRHIP3"
/db_xref="H-InvDB:HIT000490347"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/organism="Homo sapiens"
/tissue_type="hippocampus"
CDS 98..2440
/codon_start=1
/note="highly similar to Probable E3 ubiquitin-protein
ligase HECTD2"
/protein_id="BAG58564.1"
/transl_table=1
/translation="MSEAVRVPSPATPLVVAAAAPEERKGKESEREKLPPIVSAGAGA
TAGLDRGAKGQISTFSSFISAVSPKKEAAENRSSPAHLVFPNIKNVREPPPICLDVRQ
KQRTSMDASSSEMKAPVLPEPILPIQPKTVKDFQEDVEKVKSSGDWKAVHDFYLTTFD
SFPELNAAFKKDATASFNTIEDSGINAKFVNAVYDTLLNTPQDVQKTVLKGIINSLLR
EWKGPRTKDDLRAYFILLQNPQFNNTSTYVIYAHLLRQIATLVEADHHFLVHWFKKQY
SRLSQKRFKQLVERLLQFISLRLFPAKPEEFPPITKCSWWIPSAAKVLALLNTANNLV
HPPLIPYTDFYNSTLDHIDLMEEYHTWQNFGNSHRFSFCQYPFVISVAAKKIIIQRDS
EQQMINIARQSLVDKVSRRQRPDMNILFLNMKVRRTHLVSDSLDELTRKRADLKKKLK
VTFVGEAGLDMGGLTKGWFLLLIRQIFHPDYGMFTYHKDSHCHWFSSFKCDNYSEFRL
VGILMGLAVYNSITLDIRFPPCCYKKLLSPPIIPSDQNIPVGICNVTVDDLCQIMPEL
AHGLSELLSHEGNVEEDFYSTFQVFQEEFGIIKSYNLKPGGDKISVTNQNRKEYVQLY
TDFLLNKSIYKQFAAFYYGFHSVCASNALMLLRPEEVEILVCGSPDLDMHALQRSTQY
DGYAKTDLTIKYFWDVVLGFPLDLQKKLLHFTTGSDRVPVGGMADLNFKISKNETSTN
CLPVAHTCFNQLCLPPYKSKKDLKQKLIIGISNSEGFGLE"
BASE COUNT 888 a 612 c 586 g 838 t
ORIGIN
1 gcagagccct ctcgcggccg cggcggcagc agcagcgcca gccccagcaa cactgaggcc
61 gccgccgccg cctggcgctc ccgccgcccg gcccgacatg agtgaggcgg ttcgggtacc
121 ctcgcccgcc actccgctgg tggtggcggc ggccgcgcct gaggagagga aagggaagga
181 gtcagagcgc gagaagctgc cgcccatcgt atcggcgggc gccggcgcga ccgcgggttt
241 ggacagagga gccaaaggcc aaatttccac tttcagcagt tttatttcag ctgttagccc
301 gaagaaagaa gctgctgaaa acagaagttc acctgcacat cttgttttcc ctaacatcaa
361 gaatgtgaga gaaccaccac caatttgcct tgatgttaga caaaaacagc gtacatctat
421 ggatgcatca tcatccgaaa tgaaggcccc agtccttcca gaacctattc ttcctatcca
481 gcccaaaact gtgaaagact ttcaggaaga tgtagaaaaa gttaagtcat caggagattg
541 gaaagcagta catgattttt atctaacaac gtttgattct ttcccagaat taaatgctgc
601 atttaagaaa gatgccactg cctcatttaa caccattgaa gactctggga ttaatgctaa
661 atttgtgaat gctgtgtatg ataccttact taatactcct caagacgttc agaagacagt
721 attaaaggga atcattaaca gcctgttacg agaatggaaa ggtccacgaa caaaagatga
781 tcttagagca tattttatac ttttacagaa tcctcagttt aataacacat ctacgtatgt
841 catctatgct cacttgctac gacagatagc taccttagtg gaagctgacc atcatttcct
901 agttcactgg tttaaaaaac agtattcaag attgtcccag aagaggttca aacaattggt
961 agagagattg ctgcaattta tttctttacg cctgtttcct gcaaagcctg aagaatttcc
1021 acctataaca aagtgttcct ggtggattcc atcagccgct aaagtgttgg ctttacttaa
1081 tactgcaaac aatttagttc accctcccct tattccttat actgatttct ataattctac
1141 attggatcac attgatctca tggaagaata tcatacttgg caaaactttg gaaactctca
1201 caggttttcc ttctgtcagt acccattcgt tatttctgta gctgcaaaaa aaatcattat
1261 tcagagagac tcagagcaac agatgataaa catcgcaagg caaagtctgg tggataaagt
1321 atctcgaaga cagagacctg atatgaatat attatttcta aatatgaaag taagacggac
1381 acatctggtt agcgattcac ttgatgagct aacccggaaa agagccgatt tgaaaaagaa
1441 gttaaaagtt acatttgtag gggaagctgg tttggatatg ggtggtttga ctaaaggatg
1501 gttccttctt ctaattcgcc aaatttttca tccagattat ggcatgttta catatcacaa
1561 ggattcacac tgccattggt ttagcagctt taaatgtgat aactattctg aattccgatt
1621 ggttggaatt cttatgggac tagctgttta taacagcatc accttggata ttcgtttccc
1681 tccctgctgt tacaagaaat tattgagccc tcccatcatt cctagtgatc aaaatatacc
1741 agtaggcatc tgcaatgtta ccgtggacga cttatgtcaa attatgcctg agttggccca
1801 tggattaagt gaactcttat cacatgaagg caatgttgaa gaagatttct attcaacatt
1861 tcaggttttt caagaagaat ttggaataat caagtcctat aatttaaagc ccggtggtga
1921 taaaatttca gttaccaatc aaaatagaaa agaatatgta cagctttata ctgacttcct
1981 tctgaacaaa tccatctata agcagtttgc tgcattttat tatggatttc atagtgtgtg
2041 tgcttcaaat gccctaatgc tgcttcgtcc agaagaggtt gaaattttgg tctgtggaag
2101 tcctgacctg gatatgcatg ctctgcagag aagtactcag tatgatggct atgcaaaaac
2161 ggacttaact ataaaatact tttgggatgt tgtgcttgga tttcctcttg atcttcaaaa
2221 gaagttgcta cattttacta cagggagtga cagagtacct gtaggaggga tggctgattt
2281 gaactttaaa atctcaaaga atgaaacttc tactaactgc ttacctgtgg cccatacctg
2341 cttcaatcaa ctttgccttc ccccctacaa gagcaaaaag gatctgaaac agaaattgat
2401 cattggaatt tcaaattcag aaggttttgg acttgagtaa cctagaagac ttgaaatata
2461 atcttttata tgtagcattc acttccctct tactgtgcct ttagcctttt catgtttctg
2521 tctcaaaaca ctttgacaaa gctcaccaac tttaaaatat taagttttta aaaaatcaaa
2581 tatgaagtat gttcagcaaa ggcataattt tctaaaaaca cacacagaaa tcgcttgagt
2641 gaggaaaagt ccacatggca gagacaggta tggtccagtt cctcttttat atagcacttt
2701 atcattctcc acaagtagtt tgtcacaggc attccactgg gaaagcaaag tacagtgaag
2761 aatgaattta tggtatagtt gaacccagtg gcagattgta cactgctagc actgctagca
2821 cacagtagca aagcaaacag ctacattatc tttaacataa ggcatgtagc catattttca
2881 tatagtggta tacaacacag tatcattgca aatgcagttt acag
//