LOCUS AK295712 2294 bp mRNA linear HUM 24-JUL-2008
DEFINITION Homo sapiens cDNA FLJ58724 complete cds, highly similar to
Eukaryotic translation initiation factor2-alpha kinase 1 (EC
2.7.11.1).
ACCESSION AK295712
VERSION AK295712.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2294)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..2294
/clone="BRHIP3016243"
/clone_lib="BRHIP3"
/db_xref="H-InvDB:HIT000490336"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/organism="Homo sapiens"
/tissue_type="hippocampus"
CDS 320..2053
/codon_start=1
/note="highly similar to Eukaryotic translation
initiation factor2-alpha kinase 1 (EC 2.7.11.1)"
/protein_id="BAG58556.1"
/transl_table=1
/translation="MNQTHFVQDRCLSDPCEDISRIQKIRSREVALEAQTSRYLNEFE
ELAILGKGGYGRVYKVRNKLDGQYYAIKKILIKGATKTVCMKVLREVKVLAGLQHPNI
VGYHTAWIEHVHVIQPRADRAAIELPSLEVLSDQEEDREQCGVKNDESSSSSIIFAEP
TPEKEKRFGESDTENQNNKSVKYTTNLVIRESGELESTLELQENGLAGLSASSIVEQQ
LPLRRNSHLEESFTSTEESSEENVNFLGQTEAQYHLMLHIQMQLCELSLWDWIVERNK
RGREYVDESACPYVMANVATKIFQELVEGVFYIHNMGIVHRDLKPRNIFLHGPDQQVK
IGDFGLACTDILQKNTDWTNRNGKRTPTHTSRVGTCLYASPEQLEGSEYDAKSDMYSL
GVVLLELFQPFGTEMERAEVLTGLRTGQLPESLRKRCPVQAKYIQHLTRRNSSQRPSA
IQLLQSELFQNSGNVNLTLQMKIIEQEKEIAELKKQLNLLSQDKGFSVIPKPHPCKAL
YSAPQMISRQLRSLSCLCPALFPGTSSFIVALSSPANLYIPQRQRSDELVFESQKGSA
MELAVITVDGV"
BASE COUNT 668 a 530 c 559 g 537 t
ORIGIN
1 ggacggcgct agctgcagca tcggagtgtg gcagtgctgg gctggccggc gggctgggct
61 gcggcccgcg cgcggccggc gatgcagggg ggcaactccg gggtccgcaa gcgcgaagag
121 gagggcgacg gggctggggc tgtggctgcg ccgccggcca tcgactttcc cgccgagggc
181 ccggaccccg aatatgacga atctgatgtt ccagcagaaa tccaggtgtt aaaagaaccc
241 ctacaacagc caaccttccc ttttgcagtt gcaaaccaac tcttgctggt ttctttgctg
301 gagcacttga gccacgtgca tgaaccaaac ccacttcgtt caagacaggt gtttaagcga
361 tccttgtgag gatatttctc gtatccagaa aatcagatca agggaagtag ccttggaagc
421 acaaacttca cgttacttaa atgaatttga agaacttgcc atcttaggaa aaggtggata
481 cggaagagta tacaaggtca ggaataaatt agatggtcag tattatgcaa taaaaaaaat
541 cctgattaag ggtgcaacta aaacagtttg catgaaggtc ctacgggaag tgaaggtgct
601 ggcaggtctt cagcacccca atattgttgg ctatcacacc gcgtggatag aacatgttca
661 tgtgattcag ccacgagcag acagagctgc cattgagttg ccatctctgg aagtgctctc
721 cgaccaggaa gaggacagag agcaatgtgg tgttaaaaat gatgaaagta gcagctcatc
781 cattatcttt gctgagccca ccccagaaaa agaaaaacgc tttggagaat ctgacactga
841 aaatcagaat aacaagtcgg tgaagtacac caccaattta gtcataagag aatctggtga
901 acttgagtcg accctggagc tccaggaaaa tggcttggct ggtttgtctg ccagttcaat
961 tgtggaacag cagctgccac tcaggcgtaa ttcccaccta gaggagagtt tcacatccac
1021 cgaagaatct tccgaagaaa atgtcaactt tttgggtcag acagaggcac agtaccacct
1081 gatgctgcac atccagatgc agctgtgtga gctctcgctg tgggattgga tagtcgagag
1141 aaacaagcgg ggccgggagt atgtggacga gtctgcctgt ccttatgtta tggccaatgt
1201 tgcaacaaaa atttttcaag aattggtaga aggtgtgttt tacatacata acatgggaat
1261 tgtgcaccga gatctgaagc caagaaatat ttttcttcat ggccctgatc agcaagtaaa
1321 aataggagac tttggtctgg cctgcacaga catcctacag aagaacacag actggaccaa
1381 cagaaacggg aagagaacac caacacatac gtccagagtg ggtacttgtc tgtacgcttc
1441 acccgaacag ttggaaggat ctgagtatga tgccaagtca gatatgtaca gcttgggtgt
1501 ggtcctgcta gagctctttc agccgtttgg aacagaaatg gagcgagcag aagttctaac
1561 aggtttaaga actggtcagt tgccggaatc cctccgtaaa aggtgtccag tgcaagccaa
1621 gtatatccag cacttaacga gaaggaactc atcgcagaga ccatctgcca ttcagctgct
1681 gcagagtgaa cttttccaaa attctggaaa tgttaacctc accctacaga tgaagataat
1741 agagcaagaa aaagaaattg cagaactaaa gaagcagcta aacctccttt ctcaagacaa
1801 agggtttagc gtcattccaa aaccacaccc ttgcaaagct ttgtactccg caccccagat
1861 gatctccagg cagcttagat ctctttcctg cctttgccct gcactgttcc ccggtacttc
1921 ctcctttatt gtagcactca gctccccagc caatctgtac atccctcaga ggcagcgatc
1981 tgatgaattg gtttttgaat cccagaaagg gtctgccatg gagttggcag tcatcacggt
2041 agatggcgta tgattttgct gaattttaaa taaaatgaaa accataaatt acatgatgct
2101 tttattgaca cttgacaact ggcctaaata aaaagactct gactctaata caagtcccct
2161 tactgataat aggcatgaaa gagcaccatt cttaaaatct aaacccttta aaatcagtta
2221 cggcaattca cttaaggagc ttgagggccg tgttaaaagg agccagattt tcacaagacc
2281 tcatccacct ctgc
//