LOCUS AK295097 2895 bp mRNA linear HUM 24-JUL-2008
DEFINITION Homo sapiens cDNA FLJ57930 complete cds, highly similar to Homo
sapiens RAS guanyl releasing protein 1 (calcium and DAG-regulated)
(RASGRP1), mRNA.
ACCESSION AK295097
VERSION AK295097.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2895)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..2895
/clone="BRAWH3040209"
/clone_lib="BRAWH3"
/db_xref="H-InvDB:HIT000489721"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/organism="Homo sapiens"
/tissue_type="brain"
CDS 187..2436
/codon_start=1
/note="highly similar to Homo sapiens RAS guanyl
releasing protein 1 (calcium and DAG-regulated)
(RASGRP1), mRNA"
/protein_id="BAG58133.1"
/transl_table=1
/translation="MMVSLGHLAKGASLDDLIDSCIQSFDADGNLCRSNQLLQVMLTM
HRIVISSAELLQKVITLYKDALAKNSPGLCLKICYFVRYWITEFWVMFKMDASLTDTM
EEFQELVKAKGEELHCRLIDTTQINARDWSRKLTQRIKSNTSKKRKVSLLFDHLEPEE
LSEHLTYLEFKSFRRISFSDYQNYLVNSCVKENPTMERSIALCNGISQWVQLMVLSRP
TPQLRAEVFIKFIQVAQKLHQLQNFNTLMAVIGGLCHSSISRLKETSSHVPHEINKVL
GEMTELLSSSRNYDNYRRAYGECTDFKIPILGVHLKDLISLYEAMPDYLEDGKVNVHK
LLALYNHISELVQLQEVAPPLEANKDLVHLLTLSLDLYYTEDEIYELSYAREPRNHRA
PPLTPSKPPVVVDWASGVSPKPDPKTISKHVQRMVDSVFKNYDHDQDGYISQVEFEKI
AASFPFSFCVMDKDREGLISRDEITAYFMRASSIYSKLGLGFPHNFQETTYLKPTFCD
NCAGFLWGVIKQGYRCKDCGMNCHKQCKDLVVFECKKRAKNPVAPTENNTSVGPVSNL
CSLGAKDLLHAPEEGPFTFPNGEAVEHGEESKDRTIMLMGVSSQKISLRLKRAVAHKA
TQTESQPWIGSEGPSGPFVLSSPRKTAQDTLYVLPSPTSPCPSPVLVRKRAFVKWENK
DSLIKSKEELRHLRLPTYQELEQEINTLKADNDALKIQLKYAQKKIESLQLEKSNHVL
AQMEQGDCS"
BASE COUNT 814 a 728 c 634 g 719 t
ORIGIN
1 attcccttcc tctccaatct gccttcagag cgtggagctg cccgtttgtc ccctcactgc
61 cttctctctt tccccaggaa accttcccat ggctgcagag ctgcctctaa agcaagacta
121 gaggcaaagc cagccaacag ccccttcccc tcccatccca gcttggccca catcacccag
181 ttccgaatga tggtgtctct gggacattta gccaaaggag ccagcctgga cgatctcatt
241 gacagctgca ttcaatcttt tgatgcagat ggaaacctgt gtcgaagtaa ccaactgttg
301 caagtcatgc tgaccatgca ccgaattgtc atctcctctg cagaactgct ccaaaaagtt
361 atcaccctct ataaggatgc tttggcaaag aattcaccag gactttgcct gaagatctgt
421 tattttgtaa ggtattggat aacagaattc tgggtcatgt ttaaaatgga cgccagcttg
481 acagacacta tggaggagtt tcaggaactg gtgaaagcta agggtgagga gttacattgc
541 cgcctgattg acacaactca aatcaatgcc cgtgactggt ccaggaaact tactcaaagg
601 ataaaatcaa ataccagcaa gaaacggaaa gtctccctgc tctttgacca tctggaacca
661 gaagagctat ccgagcacct cacctacctt gagttcaagt ctttccggag gatatcgttc
721 tctgattatc agaattacct tgtaaatagc tgtgtgaagg aaaaccccac catggagcga
781 tctattgctc tgtgcaacgg catctcccag tgggtacaac tgatggttct cagccgcccc
841 acgccgcagc tccgagcaga agtcttcatc aagttcatcc aggtggctca gaagctccac
901 caactacaga acttcaatac actgatggct gtgataggtg ggctgtgtca cagctcaatc
961 tcgaggctca aggagacaag ttcgcatgtc ccacatgaaa tcaataaggt tctcggtgag
1021 atgactgagc tgctgtcctc ctccagaaac tacgacaatt accggcgagc ctatggagag
1081 tgcaccgact tcaagatccc cattctgggt gtgcatctca aggacctcat ctccctgtat
1141 gaagccatgc ctgactatct ggaggacggg aaagtgaacg tccataagct actggcccta
1201 tacaatcata tcagtgaatt ggtccagctg caagaggtgg ccccaccctt ggaggctaac
1261 aaggacttgg tacacttgct gacgttatcc ctggatcttt actacactga ggatgaaatc
1321 tatgagcttt cctatgcccg ggaaccaagg aaccacagag ctccaccact aacaccttca
1381 aagccaccag tagtagtgga ctgggcttct ggagtgtctc ccaaacctga tccaaaaacc
1441 attagcaaac acgtccagag gatggtggat tctgtcttca agaactatga tcacgaccag
1501 gatggataca tttctcaggt agaatttgaa aagattgctg cgagttttcc attttccttc
1561 tgtgtgatgg acaaagacag ggaaggcctc atcagcaggg atgagatcac agcctacttc
1621 atgagagcca gctcaatcta ttccaagctg ggcctgggct ttcctcacaa cttccaagag
1681 accacctacc tgaagcccac tttttgtgac aactgtgctg gatttctctg gggagtgatc
1741 aaacaaggat atcgatgtaa agactgcggg atgaactgtc acaaacaatg caaagatctg
1801 gttgtgtttg agtgtaagaa gcgagccaag aacccagtag ctcccacaga gaacaacact
1861 tctgtggggc cagtgtccaa cctttgctca ttgggagcca aagatctgct ccatgcacct
1921 gaggaaggac cttttacatt ccctaatggg gaggctgtgg aacatggtga ggagagtaag
1981 gatcggacca tcatgctgat gggagtgtcc tcacagaaga tttctcttcg gctgaagagg
2041 gctgttgccc acaaggccac ccagactgaa tcacagcctt ggattggcag tgagggccct
2101 tcaggtccct ttgtgctgtc ttccccaagg aagacagccc aggatactct atatgtgctt
2161 cccagtccca cctctccatg tcctagccca gtcttggtca gaaagcgggc ttttgtcaag
2221 tgggagaata aagactccct cataaaatca aaggaggagc tccgtcacct cagactgcct
2281 acctaccaag aactggaaca ggaaataaat actctgaaag cagataatga tgccctaaag
2341 atccaactga aatatgcaca gaagaaaata gaatccctcc agcttgaaaa aagcaatcat
2401 gtcttagctc aaatggagca gggtgactgt tcttagccca gaaactaagt agcacaatct
2461 gtagatgagt atagtgatct catttcctaa actgtaatgc acagacctga ggaactttac
2521 actgaccagc tttaaaacag tactttaaaa ggaaaagcct gttactgttt atttacctaa
2581 aagattccta atgtgcagca ctgttttctc tttcagttag ttgactcaaa gggggaaaac
2641 taaagaatgc aaaacttttg ctattcatac cactgatgtt catcaaaagt atgcgatata
2701 aaacatgact atcatttcct gacaatgggg catctcggtg gcctgcctgg ctgattcttc
2761 cttaaaacta aaatctctgg aaaatggatt tgcttcttac cctgtgtttt ctgcaaactg
2821 acttactttg ttccagccaa agcttgctaa taatagaaaa ctacccatat tccaaaagta
2881 gatttcctct gtatc
//