LOCUS AK295047 3617 bp mRNA linear HUM 24-JUL-2008
DEFINITION Homo sapiens cDNA FLJ61256 complete cds, highly similar to Guanine
nucleotide exchange factor DBS.
ACCESSION AK295047
VERSION AK295047.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3617)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..3617
/clone="BRAWH3035412"
/clone_lib="BRAWH3"
/db_xref="H-InvDB:HIT000489671"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/organism="Homo sapiens"
/tissue_type="brain"
CDS 195..3566
/codon_start=1
/note="highly similar to Guanine nucleotide exchange
factor DBS"
/protein_id="BAG58097.1"
/transl_table=1
/translation="MTVRRLSLLCRDLWALWLLLKAGADEIMHQDIVPLCAADIQDQL
KKRFAYLSGGRGQDGSPVITFPDYPAFSEIPDKEFQNVMTYLTSIPSLQDAGIGFILV
IDRRRDKWTSVKASVLRIAASFPANLQLVLVLRPTGFFQRTLSDIAFKFNRDDFKMKV
PVIMLSSVPDLHGYIDKSQLTEDLGGTLDYCHSRWLCQRTAIESFALMVKQTAQMLQS
FGTELAETELPNDVQSTSSVLCAHTEKKDKAKEDLRLALKEGHSVLESLRELQAEGSE
PSVNQDQLDNQATVQRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQGFREVKAI
LDAASQKIATFTDIGNSLAHVEHLLRDLASFEEKSGVAVERARALSLDGEQLIGNKHY
AVDSIRPKCQELRHLCDQFSAEIARRRGLLSKSLELHRRLETSMKWCDEGIYLLASQP
VDKCQSQDGAEAALQEIEKFLETGAENKIQELNAIYKEYESILNQDLMEHVRKVFQKQ
ASMEEVFHRRQASLKKLAARQTRPVQPVAPRPEALAKSPSPSPGIRRGSENSSSEGGA
LRRGPYRRAKSEMSESRQGRGSAGEEEESLAILRRHVMSELLDTERAYVEELLCVLEG
YAAGMDNPLMAHLLSTGLHNKKDVLFGNMEEIYHFHNRIFLRELENYTDCPELVGRCF
LERMEDFQIYEKYCQNKPRSESLWRQCSDCPFFQECQRKLDHKLSLDSYLLKPVQRIT
KYQLLLKEMLKYSRNCEGAEDLQEALSSILGILKAVNDSMHLIAITGYDGNLGDLGKL
LMQGSFSVWTDHKRGHTKVKELARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAPSY
SYKQSLNMAAVGITENVKGDAKKFEIWYNAREEVYIVQAPTPEIKAAWVNEIRKVLTS
QLQACREASQHRALEQSQSLPLPAPTSTSPSRGNSRNIKKLEERKTDPLSLEGYVSSA
PLTKPPEKGKGWSKTSHSLEAPEDDGGWSSAEEQINSSDAEEDGGLGPKKLVPGKYTV
VADHEKGGPDALRVRSGDVVELVQEGDEGLWYVRDPTTGKEGWVPASSLSVRLGPSGS
AQRLSSSESSPGSAVLSNSSSCSEGGQAPFSDLQG"
BASE COUNT 816 a 1035 c 1177 g 589 t
ORIGIN
1 gacatcgaat cggaggccct gggaggagca gccggctggc tgccctgcag aggccaggtc
61 tgcccagcaa acccaggaag gtgtggcgtc cccgcttcgc ggccaagatg gtgctggtgc
121 tgcgccatcc tttgtgtgcc cgggaagggc gttccgggag ccgggtcggg ggctcctgac
181 tcgcactggg cagcatgacg gtgcgccggc tgtcactgct gtgccgggac ctctgggcgc
241 tgtggctgct gctgaaggcc ggcgcagatg aaatcatgca ccaggacatc gtcccgctct
301 gtgctgccga catccaggac cagctaaaga agcgctttgc ttacctgtcc ggtgggcggg
361 ggcaggacgg aagcccggtt atcaccttcc ctgactaccc ggccttcagc gagattccgg
421 acaaggagtt ccagaatgtc atgacctacc tcaccagcat ccccagcctg caggacgctg
481 gcatcggatt catcctggtg atagaccggc gacgggacaa atggacctcc gtgaaggcgt
541 ccgtcctgcg catcgcagca tctttcccgg caaacctgca gctcgtcctc gtgcttcgcc
601 cgacgggttt tttccaaagg actctctccg acatcgcttt caaattcaat agagatgact
661 ttaagatgaa ggtgccggtc ataatgctga gctccgtacc agacttacac ggttacatcg
721 ataagtcgca gctgaccgag gacctgggtg ggaccctgga ctactgccac tcccggtggc
781 tgtgccagcg cacggccatc gaaagtttcg ccctcatggt gaagcagacg gctcagatgc
841 tgcagtcctt cgggaccgag ctggctgaaa cagagctgcc caatgacgtc cagtcgacaa
901 gctcagtgct gtgtgcgcac acagagaaga aggacaaggc gaaggaggat ttgaggctgg
961 cactgaaaga ggggcacagt gtcctggaga gcctcaggga gctgcaggct gagggctcag
1021 agcccagtgt gaaccaggac cagcttgaca accaggccac cgtgcagagg ctcctggccc
1081 agctgaacga aaccgaggct gccttcgatg agttctgggc aaagcatcag cagaaactgg
1141 agcagtgtct gcagctccgg cactttgagc agggcttccg ggaggtcaaa gccatcttgg
1201 acgcagcgtc ccagaagata gcaaccttca cagacatcgg caacagcctg gcgcatgtgg
1261 aacacctgct gagggacctg gccagcttcg aggagaaatc aggcgtggcc gtggagaggg
1321 cccgggccct gtctctggac ggcgagcagc tcattgggaa caagcactac gcggtagact
1381 ccatccgccc aaagtgccag gagctccggc acctctgtga ccagttctct gcggagatcg
1441 caaggaggag ggggctgctc agcaagtccc tggagctgca ccgccgcctg gagacgtcca
1501 tgaagtggtg tgatgaaggg atttacctgc tggcctcaca acctgtggac aagtgccagt
1561 cccaggacgg cgcggaggct gccctccagg aaatcgagaa gtttttggag accggtgcgg
1621 aaaataagat ccaggagctc aacgcgattt acaaggaata cgaatccatc ctcaaccaag
1681 atctcatgga gcacgtgcga aaggtcttcc agaagcaggc aagcatggag gaggtgttcc
1741 accgcaggca ggccagcctg aagaagctgg cggccaggca gacgcggccc gtgcagccgg
1801 tggcccccag acccgaggca ctggcaaagt cgccctcccc ctccccaggc attcggcgag
1861 gctctgagaa ctccagctcc gagggcggtg cgctccggag agggccctac cggagggcca
1921 agagtgagat gagtgagagc cggcagggcc gcggctcagc gggggaggag gaggaaagcc
1981 tggccatcct gcgcaggcac gtgatgagcg agctcctgga cacagaacgg gcctacgtgg
2041 aggagctgct gtgcgtcctg gagggctacg ccgcggggat ggataaccca ctgatggctc
2101 acctcctgtc aacaggcctt cacaacaaga aggatgtttt gtttggaaac atggaggaaa
2161 tctatcactt ccacaacagg atattcctca gggagctgga aaactacact gactgcccag
2221 aactggttgg aagatgcttt ctggagagga tggaagattt ccagatctat gagaagtact
2281 gtcagaacaa gccccgctct gagagcctgt ggagacagtg ctccgactgc ccgtttttcc
2341 aggaatgcca gagaaagctg gaccacaagc tgagcctgga ctcctacctg ctgaagccag
2401 tgcagaggat caccaagtac cagctgctgc tcaaggaaat gctgaaatac agcaggaact
2461 gcgagggggc tgaggacctg caggaggcgc tgagctccat cctgggcatc ctgaaggccg
2521 tgaacgactc catgcacctc atcgctatca ccggctatga cgggaatctc ggcgacctgg
2581 gcaagctgct gatgcagggc tcgttcagcg tctggaccga ccacaagagg ggccacacca
2641 aggtgaagga gctggccagg ttcaagccca tgcagcggca cctgttcctg cacgagaagg
2701 cagtgctctt ctgcaagaag agggaggaga atggggaggg gtatgagaaa gctccctcct
2761 acagctacaa gcagtcctta aacatggctg ccgttggcat tacggagaac gtgaagggag
2821 atgctaagaa gttcgagatc tggtacaacg cgcgcgagga ggtctacatc gtccaggcgc
2881 caactcctga gattaaagcc gcgtgggtga atgaaattcg gaaagtgctg accagccagc
2941 tgcaagcttg tagagaagcc agccagcacc gggcgctgga gcagtcacag agcctgcccc
3001 tgccggcccc gaccagcacc agtccctcaa gaggaaactc aaggaacatc aagaagctgg
3061 aagaaaggaa aacagacccc ctaagcctgg agggatacgt cagctcagcg ccactgacaa
3121 agccccccga aaagggcaaa ggttggagca aaacgtccca ctcactggag gcacctgagg
3181 acgacggggg ctggtcaagt gcagaggagc agattaactc gtccgacgca gaggaggacg
3241 gcgggttggg ccccaagaag ctggttccag gtaaatacac ggtcgtggcg gaccacgaga
3301 agggaggccc cgatgcgctg cgcgtgagga gcggggacgt ggtggagctg gtgcaggagg
3361 gcgacgaggg cctctggtac gtcagggacc cgaccactgg caaggagggc tgggtgccgg
3421 ccagcagcct gtccgtccgg ctcggcccgt ccggctcggc ccagcgcctg agcagctcag
3481 agtcgagccc ggggtcggcc gtgctgagca actcgtccag ctgcagcgag ggcggccagg
3541 cccccttctc cgacctgcag gggtagcgcg gcctcggcgc cggagacccg cgcgctgtct
3601 ggggctgcgg tggcgtg
//