LOCUS AK294918 5169 bp mRNA linear HUM 24-JUL-2008
DEFINITION Homo sapiens cDNA FLJ58788 complete cds, highly similar to Homo
sapiens HECT, C2 and WW domain containing E3 ubiquitin protein
ligase 1 (HECW1), mRNA.
ACCESSION AK294918
VERSION AK294918.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 5169)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..5169
/clone="BRAWH3024512"
/clone_lib="BRAWH3"
/db_xref="H-InvDB:HIT000489542"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/organism="Homo sapiens"
/tissue_type="brain"
CDS 325..5043
/codon_start=1
/note="highly similar to Homo sapiens HECT, C2 and WW
domain containing E3 ubiquitin protein ligase 1 (HECW1),
mRNA"
/protein_id="BAG58003.1"
/transl_table=1
/translation="MLLHLCSVKNLYQNRFLGLAAMASPSRNSQSRRRCKEPLRYSYN
PDQFHNMDLRGGPHDGVTIPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIHWDI
KEEVDAGDWIGMYLIDEVLSENFLDYKNRGVNGSHRGQIIWKIDASSYFVEPETKICF
KYYHGVSGALRATTPSVTVKNSAAPIFKSIGADETVQGQGSRRLISFSLSDFQAMGLK
KGMFFNPDPYLKISIQPGKHSIFPALPHHGQERRSKIIGNTVNPIWQAEQFSFVSLPT
DVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSG
QLQFRFEITSSIHPDDEEISLSTEPESAQIQDSPMNNLMESGSGEPRSEAPESSESWK
PEQLGEGSVPDGPGNQSIELSRPAEEAAVITEAGDQGMVSVGPEGAGELLAQVQKDIQ
PAPSAEELAEQLDLGEEASALLLEDGEAPASTKEEPLEEEATTQSRAGREEEEKEQEE
EGDVSTLEQGEGRLQLRASVKRKSRPCSLPVSELETVIASACGDPETPRTHYIRIHTL
LHSMPSAQGGSAAEEEDGAEEESTLKDSSEKDGLSEVDTVAADPSALEEDREEPEGAT
PGTAHPGHSGGHFPSLANGAAQDGDTHPSTGSESDSSPRQGGDHSCEGCDASCCSPSC
YSSSCYSTSCYSSSCYSASCYSPSCYNGNRFASHTRFSSVDSAKISESTVFSSQDDEE
EENSAFESVPDSMQSPELDPESTNGAGPWQDELAAPSGHVERSPEGLESPVAGPSNRR
EDWEARIDSHGRVFYVDHVNRTTTWQRPTAAATPDGMRRSGSIQQMEQLNRRYQNIQR
TIATERSEEDSGSQSCEQAPAGGGGGGGSDSEAESSQSSLDLRREGSLSPVNSQKITL
LLQSPAVKFITNPEFFTVLHANYSAYRVFTSSTCLKHMILKVRRDARNFERYQHNRDL
VNFINMFADTRLELPRGWEIKTDQQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTH
RQHLQRLRSYSAGEASEVSRNRGASLLARPGHSLVAAIRSQHQHESLPLAYNDKIVAF
LRQPNIFEMLQERQPSLARNHTLREKIHYIRTEGNHGLEKLSCDADLVILLSLFEEEI
MSYVPLQAAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKL
EAKGFGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYVTFVGEEGLDYSGPS
REFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENHLEWFRFSGRILGLALIH
QYLLDAFFTRPFYKALLRLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEE
VFGQVTERELKSGGANTQVTEKNKKEYIERMVKWRVERGVVQQTEALVRGFYEVVDSR
LVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHLVIRWFWAAVERFNNEQRL
RLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSY
SMLYEKLLTAVEETSTFGLE"
BASE COUNT 1218 a 1478 c 1468 g 1005 t
ORIGIN
1 gcgcatcagg cgctgttgtt ggagccggaa caccgtgcga ctctgaccga accggccccc
61 tcctcgcgca cacactcgcc gagccgcgcg cgcccctccg ccgtgacagt ggccgtggcc
121 tccgctctct cggggcaccc ggcagccaga gcgcagcgag agcgggcggt cgccagggtc
181 ccctccccag ccagtcccag gcgcccggtg cactatgcgg ggcacgtgcg ccccccagct
241 ctaatctgcg cgctgacagg agcatgatct gtgcccaggc cagggctgcc aaggaattga
301 tgcgcgtaca cgtggtgggt cattatgctg ctacacctgt gtagtgtgaa gaatctgtac
361 cagaacaggt ttttaggcct ggccgccatg gcgtctcctt ctagaaactc ccagagccga
421 cgccggtgca aggagccgct ccgatacagc tacaaccccg accagttcca caacatggac
481 ctcaggggcg gcccccacga tggcgtcacc attccccgct ccaccagcga cactgacctg
541 gtcacctcgg acagccgctc cacgctcatg gtcagcagct cctactattc catcgggcac
601 tctcaggacc tggtcatcca ctgggacata aaggaggaag tggacgctgg ggactggatt
661 ggcatgtacc tcattgatga ggtcttgtcc gaaaactttc tggactataa aaaccgtgga
721 gtcaatggtt ctcatcgggg ccagatcatc tggaagatcg atgccagctc gtactttgtg
781 gaacctgaaa ctaagatctg cttcaaatac taccatggag tgagtggggc cctgcgagca
841 accaccccca gtgtcacggt caaaaactcg gcagctccta tttttaaaag cattggtgct
901 gatgagaccg tccaaggaca aggaagtcgg aggctgatca gcttctctct ctcagatttc
961 caagccatgg ggttgaagaa agggatgttt ttcaacccag acccttatct gaagatttcc
1021 attcagcctg ggaaacacag catcttcccc gccctccctc accatggaca ggagaggaga
1081 tccaagatca taggcaacac cgtgaacccc atctggcagg ccgagcaatt cagttttgtg
1141 tccttgccca ctgacgtgct ggaaattgag gtgaaggaca agtttgccaa gagccgcccc
1201 atcatcaagc gcttcttggg aaagctgtcg atgcccgttc aaagactcct ggagagacac
1261 gccatagggg atagggtggt cagctacaca cttggccgca ggcttccaac agatcatgtg
1321 agtggacagc tgcaattccg atttgagatc acttcctcca tccacccaga tgatgaggag
1381 atttccctga gtaccgagcc tgagtcagcc caaattcagg acagccccat gaacaacctg
1441 atggaaagcg gcagtgggga acctcggtct gaggcaccag agtcctctga gagctggaag
1501 ccagagcagc tgggtgaggg cagtgtcccc gatggtccag ggaaccaaag catagagctt
1561 tccagaccag ctgaggaagc agcagtcatc acggaggcag gagaccaggg catggtctct
1621 gtgggacctg aaggggctgg ggagctcctg gcccaggtgc aaaaggacat ccagcctgcc
1681 cccagtgcag aagagctggc cgagcagctg gacctgggtg aggaggcatc agcactgctg
1741 ctggaagacg gtgaagcccc agccagcacc aaggaggagc ccttggagga ggaagcaacg
1801 acccagagcc gggctggaag ggaagaagag gagaaggagc aggaggagga gggagatgtg
1861 tctaccctgg agcagggaga gggcaggctg cagctgcggg cctcggtgaa gagaaaaagc
1921 aggccctgct ccttgcctgt gtccgagctg gagacggtga tcgcgtcagc ctgcggggac
1981 cccgagaccc cgcggacaca ctacatccgc atccacaccc tgctgcacag catgccctcc
2041 gcccagggcg gcagcgcggc agaggaggag gacggcgcgg aggaggagtc caccctcaag
2101 gactcctcgg agaaggatgg gctcagcgag gtggacacgg tggccgctga cccgtctgcc
2161 ctggaagagg acagagaaga gcccgagggg gctactccag gcacggcgca ccctggccac
2221 tccgggggcc acttccccag cctggccaat ggcgcggccc aggatggcga cacgcacccc
2281 agcaccggga gcgagagcga ctccagcccc aggcaaggcg gggaccacag ttgcgagggc
2341 tgtgacgcgt cctgctgcag cccctcgtgc tacagctcct cgtgctacag cacgtcctgc
2401 tacagcagct cgtgctacag cgcctcgtgc tacagcccct cctgctacaa cggcaacagg
2461 ttcgccagcc acacgcgctt ctcctccgtg gacagcgcca agatctccga gagcacggtc
2521 ttctcctcgc aagacgacga ggaggaggag aacagcgcgt tcgagtcggt acccgactcc
2581 atgcagagcc ctgagctgga cccggagtcc acgaacggcg ctgggccgtg gcaagacgag
2641 ctggccgccc ctagcgggca cgtggaaaga agcccggaag gtctggaatc ccccgtggca
2701 ggtccaagca atcggagaga agactgggaa gctcgaattg acagccacgg gcgggtcttt
2761 tatgtggacc acgtgaaccg cacaaccacc tggcagcgtc cgacggcagc agccaccccg
2821 gatggcatgc ggagatcggg gtccatccag cagatggagc aactcaacag gcggtatcaa
2881 aacattcagc gaaccattgc aacagagagg tccgaagaag attctggcag ccaaagctgc
2941 gagcaagccc cagcaggagg aggcggaggt ggagggagtg actcagaagc cgaatcttcc
3001 cagtccagct tagatctaag gagagagggg tcactttctc cagtgaactc acaaaaaatc
3061 accttgctgc tgcagtcccc agcggtcaag ttcatcacca accccgagtt cttcactgtg
3121 ctacacgcca attatagtgc ctaccgagtc ttcaccagta gcacctgctt aaagcacatg
3181 attctgaaag tccgacggga tgctcgcaat tttgaacgct accagcacaa ccgggacttg
3241 gtgaatttca tcaacatgtt cgcagacact cggctggaac tgccccgggg ctgggagatc
3301 aaaacggacc agcagggaaa gtcttttttc gtggaccaca acagtcgagc taccactttc
3361 attgaccccc gaatccctct tcagaacggt cgtcttccca atcatctaac tcaccgacag
3421 cacctccaga ggctccgaag ttacagcgcc ggagaggcct cagaagtttc tagaaacaga
3481 ggagcctctt tactggccag gccaggacac agcttagtag ctgctattcg aagccaacat
3541 caacatgagt cattgccact ggcatataat gacaagattg tggcatttct tcgccagcca
3601 aacatttttg aaatgctgca agagcgtcag ccaagcttag caagaaacca cacactcagg
3661 gagaaaatcc attacattcg gactgagggt aatcacgggc ttgagaagtt gtcctgtgat
3721 gcggatctgg tcattttgct gagtctcttt gaagaagaga ttatgtccta cgtccccctg
3781 caggctgcct tccaccctgg gtatagcttc tctccccgat gttcaccctg ttcttcacct
3841 cagaactccc caggtttaca gagagccagt gcaagagccc cttcccccta ccgaagagac
3901 tttgaggcca agctccgcaa tttctacaga aaactggaag ccaaaggatt tggtcagggt
3961 ccggggaaaa ttaagctcat tattcgccgg gatcatttgt tggagggaac cttcaatcag
4021 gtgatggcct attcgcggaa agagctccag cgaaacaagc tctacgtcac ctttgttgga
4081 gaggagggcc tggactacag tggcccctcg cgggagttct tcttccttct gtctcaggag
4141 ctcttcaacc cttactatgg actctttgag tactcggcaa atgatactta cacggtgcag
4201 atcagcccca tgtccgcatt tgtagaaaac catcttgagt ggttcaggtt tagcggtcgc
4261 atcctgggtc tggctctgat ccatcagtac cttcttgacg ctttcttcac gaggcccttc
4321 tacaaggcac tcctgagact gccctgtgat ttgagtgacc tggaatattt ggatgaggaa
4381 ttccaccaga gtttgcagtg gatgaaggac aacaacatca cagacatctt agacctcact
4441 ttcactgtta atgaagaggt ttttggacag gtcacggaaa gggagttgaa gtctggagga
4501 gccaacacac aggtgacgga gaaaaacaag aaggagtaca tcgagcgcat ggtgaagtgg
4561 cgggtggagc gcggcgtggt acagcagacc gaggcgctgg tgcgcggctt ctacgaggtt
4621 gtagactcga ggctggtgtc cgtgtttgat gccagggagc tggagctggt gatagctggc
4681 accgcggaaa tcgacctaaa tgactggcgg aataacactg agtaccgggg aggttaccac
4741 gatgggcatc ttgtgatccg ctggttctgg gctgcggtgg agcgcttcaa taatgagcag
4801 aggctgagat tactgcagtt tgtcacggga acatccagcg tgccctacga aggcttcgca
4861 gccctccgtg ggagcaatgg gcttcggcgc ttctgcatag agaaatgggg gaaaattact
4921 tctctcccca gggcacacac atgcttcaac cgactggatc ttccaccgta tccctcgtac
4981 tccatgttgt atgaaaagct gttaacagca gtagaggaaa ccagcacctt tggacttgag
5041 tgaggacatg gaacctcgcc tgacattttc ctggccagtg acatcaccct tcctgggatg
5101 atcccctttt ccctttccct taatcaactc tcctttgatt ttggtattcc atgattttta
5161 ttttcaaac
//