LOCUS AK294914 2644 bp mRNA linear HUM 21-JAN-2009
DEFINITION Homo sapiens cDNA FLJ50956 complete cds, highly similar to Rap
guanine nucleotide exchange factor 4.
ACCESSION AK294914
VERSION AK294914.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2644)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..2644
/clone="BRAWH3023946"
/clone_lib="BRAWH3"
/db_xref="H-InvDB:HIT000489538"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/organism="Homo sapiens"
/tissue_type="brain"
CDS 165..2540
/codon_start=1
/note="highly similar to Rap guanine nucleotide exchange
factor 4"
/protein_id="BAH11923.1"
/transl_table=1
/translation="MIRDRKYHLKTYRQCCVGTELVDWMMQQTPCVHSRTQAVGMWQV
LLEDGVLNHVDQEHHFQDKYLFYRFLDDEHEDAPLPTEEEKKECDEELQDTMLLLSQM
GPDAHMRMILRKPPGQRTVDDLEIIYEELLHIKALSHLSTTVKRELAGVLIFESHAKG
GTVLFNQGEEGTSWYIILKGSVNVVIYGKGVVCTLHEGDDFGKLALVNDAPRAASIVL
REDNCHFLRVDKEDFNRILRDVEANTVRLKEHDQDVLVLEKVPAGNRASNQGNSQPQQ
KYTVMSGTPEKILEHFLETIRLEATLNEATDSVLNDFIMMHCVFMPNTQLCPALVAHY
HAQPSQGTEQEKMDYALNNKRRVIRLVLQWAAMYGDLLQEDDVSMAFLEEFYVSVSDD
ARMIAALKEQLPELEKIVKQISEDAKAPQKKHKVLLQQFNTGDERAQKRQPIRGSDEV
LFKVYCMDHTYTTIRVPVATSVKEVISAVADKLGSGEGLIIVKMSSGGEKVVLKPNDV
SVFTTLTINGRLFACPREQFDSLTPLPEQEGPTVGTVGTFELMSSKDLAYQMTIYDWE
LFNCVHELELIYHTFGRHNFKKTTANLDLFLRRFNEIQFWVVTEICLCSQLSKRVQLL
KKFIKIAAHCKEYKNLNSFFAIVMGLSNVAVSRLALTWEKLPSKFKKFYAEFESLMDP
SRNHRAYRLTVAKLEPPLIPFMPLLIKDMTFTHEGNKTFIDNLVNFEKMRMIANTART
VRYYRSQPFNPDAAQANKNHQDVRSYVRQLNVIDNQRTLSQMSHRLEPRRP"
BASE COUNT 778 a 589 c 615 g 662 t
ORIGIN
1 agcaaaattt caattccact ttaccggttg attatacaat tcatggcctc tctctgtctc
61 tttttcctcc tttagggatt cctgacaagg agaacgtccc ttcagagaag atcctcagag
121 ctggaaaaat tttacgaaat gccattctct ctcgagcacc tcacatgata agagatagaa
181 aataccacct aaagacatac agacaatgct gtgtgggaac tgaactggtg gactggatga
241 tgcagcagac accatgtgtt cactcccgga ctcaagctgt tggcatgtgg caagtcctgt
301 tagaagatgg tgttctcaac cacgtggacc aggagcacca tttccaagac aaatatttat
361 tctatcgatt tctggatgat gagcacgagg atgccccttt gcctactgag gaggagaaga
421 aggagtgtga tgaggagctc caggacacca tgctgctgct gtcacagatg ggccccgacg
481 cccacatgag gatgatcctt cgcaaaccac ctggccagag gactgtggat gacctagaga
541 ttatctatga ggagcttctt catattaaag ccttatccca tctttctacc acagtgaaac
601 gagagttagc aggtgttctc atttttgagt ctcacgccaa aggagggact gtgttgttta
661 accaggggga agaaggtacc tcctggtaca ttattctaaa aggatcagtg aatgtagtca
721 tttacggcaa gggtgtggtc tgcaccctgc atgaaggaga tgacttcggc aagttagcac
781 tagtgaatga tgccccacga gctgcctcta tcgtcttacg agaagataac tgccatttct
841 taagagtaga caaggaggat ttcaaccgga tcctaaggga cgtggaggcg aatacagtca
901 gacttaaaga acatgaccaa gatgtcttgg tgctggagaa ggtcccagca gggaacagag
961 cttctaatca aggaaactca cagcctcagc aaaagtatac tgtgatgtca ggaacacctg
1021 aaaaaatttt agagcatttt ctagaaacaa tacgccttga ggcaacttta aatgaagcaa
1081 cagattctgt tttaaatgac tttattatga tgcactgtgt ttttatgcca aatacccagc
1141 tttgcccggc actggtggcc cactaccacg cacagccttc acaaggtaca gaacaggaga
1201 aaatggatta tgccctcaac aataagaggc gagtcatccg cctggttcta cagtgggctg
1261 ccatgtatgg agacctcctg caagaggatg acgtgtctat ggccttcctg gaggagtttt
1321 atgtatctgt atcagatgac gcccggatga ttgctgccct caaggagcaa ctgccagagt
1381 tggagaagat tgtcaagcaa atctcagaag atgcaaaggc accacaaaag aagcacaagg
1441 ttcttttgca acagttcaat acgggcgatg agagagccca gaagcgccag cctatccgcg
1501 gctctgatga agttctgttt aaggtctatt gcatggacca cacctacaca accattcggg
1561 tgccagtggc cacttcggtg aaggaagtca tcagtgcagt tgccgacaag ctgggctccg
1621 gggagggcct gatcatagtc aagatgagtt ccggaggaga aaaggtggtg ctcaaaccta
1681 atgatgtttc agtatttacg acgctcacca ttaatggacg cctgtttgct tgcccgcgag
1741 agcaattcga ttcactgact cccttaccag aacaggaagg cccaactgtt ggaacagtgg
1801 gaacttttga actgatgagc tccaaagatt tagcatacca gatgacaatt tatgattggg
1861 aactcttcaa ctgcgtgcat gagctggagc taatctatca cacatttgga aggcataatt
1921 ttaaaaagac tacagcaaac ttggatttgt tcctgaggag atttaatgaa attcagtttt
1981 gggtcgtcac tgagatctgc ctttgttctc agctcagcaa gcgtgttcag ctattaaaaa
2041 aatttattaa gatagcagcc cactgtaagg agtataaaaa tctgaattcc ttttttgcca
2101 tcgtcatggg actaagtaac gttgctgtga gccgcttggc actaacgtgg gagaaactgc
2161 caagcaagtt caagaagttc tatgcggagt ttgaaagttt aatggaccct tcaaggaacc
2221 acagggccta caggctgaca gtagctaagc tggaacctcc tctcatcccc ttcatgcctt
2281 tgctcattaa agatatgaca tttactcatg aggggaacaa gacgttcatt gacaatctag
2341 taaactttga aaaaatgcgc atgattgcaa atacggccag aacagtgaga tactacagga
2401 gccaaccctt caatcctgat gcagctcaag ctaataagaa ccatcaggat gtccggagtt
2461 atgtacggca attaaatgtg attgacaacc agagaacttt atcacagatg tcacacagat
2521 tagagcctcg tcgaccatag acatttcaaa tgcccaaagc aacagtttgt ctccagtcca
2581 caatctttca aaaatgccat ttatgctact actgactgta ttgccactag agaattctac
2641 aaaa
//