LOCUS AK294875 3381 bp mRNA linear HUM 21-JAN-2009
DEFINITION Homo sapiens cDNA FLJ59684 complete cds, highly similar to Homo
sapiens pleckstrin homology domain containing, family G (with
RhoGef domain) member 5, transcript variant 1, mRNA.
ACCESSION AK294875
VERSION AK294875.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3381)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..3381
/clone="BRAWH3020110"
/clone_lib="BRAWH3"
/db_xref="H-InvDB:HIT000489499"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/organism="Homo sapiens"
/tissue_type="brain"
CDS 66..3323
/codon_start=1
/note="highly similar to Homo sapiens pleckstrin homology
domain containing, family G (with RhoGef domain) member
5, transcript variant 1, mRNA"
/protein_id="BAH11909.1"
/transl_table=1
/translation="MSLGERGSPQTFGGSSVSKGSDAGHDQSPAEKKGLRCQNPACMD
KGRAAKVCHHADCQQLHRRGPLNLCEACDSKFHSTMHYDGHVRFDLPPQGSVLARNVS
TRSCPPRTSPAVDLEEEEEESSVDGKGDRKSTGLKLSKKKARRRHTDDPSKECFTLKF
DLNVDIETEIVPAMKKKSLGEVLLLVFERKGIALGKVDIYLDQSNTPLSLTFEAYRFG
GHYLRVKAPAKPGDEGKVEQGMKDSKSLSLPILRPAGTGPPALERVDAQSRRESLDIL
APGRRRKNMSEFLGEASIPGQEPPTPSSCSLPSGSSGSTNTGDSWKNRAASRFSGFFS
SGPSTSAFGREVDKMEQLEGKLHTYSLFGLPRLPRGLRFDHDSWEEEYDEDEDEDNAC
LRLEDSWRELIDGHEKLTRRQCHQQEAVWELLHTEASYIRKLRVIINLFLCCLLNLQE
SGLLCEVEAERLFSNIPEIAQLHRRLWASVMAPVLEKARRTRALLQPGDFLKGFKMFG
SLFKPYIRYCMEEEGCMEYMRGLLRDNDLFRAYITWAEKHPQCQRLKLSDMLAKPHQR
LTKYPLLLKSVLRKTEEPRAKEAVVAMIGSVERFIHHVNACMRQRQERQRLAAVVSRI
DAYEVVESSSDEVDKLLKEFLHLDLTAPIPGASPEETRQLLLEGSLRMKEGKDSKMDV
YCFLFTDLLLVTKAVKKAERTRVIRPPLLVDKIVCRELRDPGSFLLIYLNEFHSAVGA
YTFQASGQALCRGWVDTIYNAQNQLQQLRAQEPPGSQQPLQSLEEEEDEQEEEEEEEE
EEEEGEDSGTSAASSPTIMRKSSGSPDSQHCASDGSTETLAMVVVEPGDTLSSPEFDS
GPFSSQSDETSLSTTASSATPTSELLPLGPVDGRSCSMDSAYGTLSPTSLQDFVAPGP
MAELVPRAPESPRVPSPPPSPRLRRRTPVQLLSCPPHLLKSKSEASLLQLLAGAGTHG
TPSAPSRSLSELCLAVPAPGIRTQGSPQEAGPSWDCRGAPSPGSGPGLVGCLAGEPAG
SHRKRCGDLPSGASPRVQPEPPPGVSAQHRKLTLAQLYRIRTTLLLNSTLTASEV"
BASE COUNT 682 a 1074 c 1088 g 537 t
ORIGIN
1 accgcggtgc actccagccc ccaggaagag aggcagcttg gagggggcca aattcagggt
61 caaagatgag tcttggggaa cgggggtcgc cccagacctt cgggggctcc tctgtctcta
121 aagggagcga tgcagggcac gaccagagcc ccgctgaaaa gaagggactg cgctgtcaga
181 accccgcctg catggacaag gggcgggcgg ccaaggtatg tcaccacgcc gactgccagc
241 agctgcaccg ccgggggccc ctcaacctct gcgaggcctg tgacagcaag ttccacagca
301 ccatgcatta tgatgggcat gtccgcttcg accttccccc acaaggctct gtgctggccc
361 ggaacgtgtc cacccggtca tgcccgccgc gcaccagccc cgcagtggac ttggaggagg
421 aggaggagga gagctctgtg gatggcaaag gggaccggaa gagcacaggc ctgaaactct
481 ccaagaagaa agcaaggagg agacacacgg atgacccaag caaggaatgc ttcactctga
541 aatttgacct gaatgtggac attgagacag agatcgtccc agccatgaag aagaagtcac
601 tgggggaggt gctgctgctt gtatttgaaa ggaagggcat tgcgctgggc aaagtggaca
661 tctacctgga ccagtccaac acacccctgt ccctcacctt cgaggcctac aggttcgggg
721 gacactacct tcgtgtcaaa gccccagcca agcctggaga tgagggcaag gtggagcagg
781 gcatgaagga ctccaagtcc ctgagtttgc cgattctgcg gccagctggg accgggcccc
841 ccgccctgga gcgtgtggac gcccagagcc gccgggagag cctggacatc ttggcccctg
901 gccgccgccg caagaacatg tcggagttcc tgggggaggc gagcatcccc gggcaggagc
961 cccccacgcc ctccagctgc tctctgccca gcggcagcag tggcagcacc aacactggcg
1021 acagctggaa gaaccgggcg gccagtcgct tcagcggctt tttcagctcc ggccccagca
1081 ccagcgcctt tggccgggag gtagacaaga tggagcagct ggagggcaag ctgcacacct
1141 acagcctctt cgggctgccc aggctgcccc gggggctgcg cttcgaccat gactcctggg
1201 aggaggagta cgatgaagac gaggatgagg acaatgcctg cctgaggctg gaggacagct
1261 ggcgggagct cattgatggg catgagaagc tgacccggcg gcagtgccac cagcaggagg
1321 cggtgtggga gctgctgcac acggaggcct cctacatcag gaaactgcgg gtgatcatca
1381 acctgttcct gtgctgcctc ctgaacctgc aagagtcagg gctgctgtgt gaggtggagg
1441 cggagcgcct gttcagcaac atcccggaga tcgcgcagct gcaccgcagg ctgtgggcta
1501 gcgtgatggc gccggtgctg gagaaggcgc ggcgcacgcg agcgctgcta cagcccgggg
1561 acttcctcaa aggcttcaag atgttcggct cgctcttcaa gccctacatc cgctactgca
1621 tggaggagga gggctgcatg gagtacatgc gcggcctgct gcgcgacaac gacctcttcc
1681 gggcctacat cacgtgggcg gagaagcacc cacagtgcca gaggctgaag ctgagcgaca
1741 tgctggccaa accccaccag cggctcacca agtacccgct gctgctcaag tcggtgctga
1801 ggaagaccga ggagccgcgc gccaaggagg ccgtcgtcgc catgatcggc tccgtggagc
1861 gcttcatcca ccacgtgaac gcgtgcatgc ggcagcggca ggagcggcag cggctggcgg
1921 ccgtggtgag ccgcatcgac gcctacgagg tggtggaaag cagcagcgac gaagtggaca
1981 agctcctgaa ggaatttctg cacctggact tgacagcgcc catccctggc gcctccccgg
2041 aggagacgcg gcagctgctg ctggagggga gcctgaggat gaaggagggg aaggacagca
2101 agatggatgt gtactgcttc ctcttcacgg atctgctgtt ggtgaccaaa gcagtgaaga
2161 aggcagagag gaccagggtc atcaggccac ccctgctcgt ggacaagatt gtgtgccggg
2221 agctacggga ccctgggtcc ttcctcctta tctacctgaa tgagtttcac agtgctgtag
2281 gggcctacac gttccaggcc agtggccagg ccttgtgccg tggctgggtg gacaccattt
2341 acaatgccca gaaccagctg caacagctgc gtgcacagga gcccccaggc agtcagcagc
2401 ccctgcagag cctggaagag gaggaggatg agcaggagga ggaagaggag gaggaggagg
2461 aggaggagga aggcgaggac agtggcactt cagctgccag ctcccctacc atcatgcgga
2521 aaagcagcgg cagccccgac tctcagcact gtgcctcaga tggctccacg gagaccctgg
2581 ccatggttgt ggtagagcct ggggacacgc tgtcctcccc cgagttcgac agcggtcctt
2641 tcagctccca gtctgatgag acctctctca gcaccactgc ctcatctgcc acgcccacca
2701 gtgagctgct gcccctgggt ccggtggacg gccgctcctg ctccatggac tctgcctacg
2761 gcaccctctc cccaacctcc ttacaagact ttgtggcccc aggcccaatg gcagagctag
2821 tgcctcgggc cccagagtcc ccacgagttc cttcccctcc accctcgccc cgtctccgcc
2881 gccgcacccc tgtccagctg ttgagctgcc cgccccacct gctcaagtct aagtccgagg
2941 ccagcctcct ccagctgctg gcaggggctg gcacccatgg gacaccctct gcccccagcc
3001 gcagcctgtc agagctctgc ctggctgttc cagccccagg tattaggact cagggctccc
3061 ctcaggaagc tgggcccagc tgggattgcc gaggggcccc tagccctggc agcggtcctg
3121 ggctagtcgg ctgcctggcc ggggaacctg caggctccca caggaagagg tgtggagacc
3181 tgccctcggg ggcctctccc agggtccagc ctgagccccc accaggggtc tctgcccagc
3241 acaggaagct gaccctggcc cagctctacc gaatcaggac caccctgctg cttaactcca
3301 cgctcactgc ctcggaggtc tgagcagagg gaggccccca agagtgccat tgaccaagag
3361 acagcagaca gcctgcctcc t
//