LOCUS AK294728 3504 bp mRNA linear HUM 31-JUL-2008
DEFINITION Homo sapiens cDNA FLJ61239 complete cds, highly similar to
Connector enhancer of kinase suppressor of ras 2.
ACCESSION AK294728
VERSION AK294728.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3504)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..3504
/clone="BRAWH3003586"
/clone_lib="BRAWH3"
/db_xref="H-InvDB:HIT000489352"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/organism="Homo sapiens"
/tissue_type="brain"
CDS 443..2992
/codon_start=1
/note="highly similar to Connector enhancer of kinase
suppressor of ras 2"
/protein_id="BAG57875.1"
/transl_table=1
/translation="MALIMEPVSKWSPSQVVDWMKGLDDCLQQYIKNFEREKISGDQL
LRITHQELEDLGVSRIGHQELILEAVDLLCALNYGLETENLKTLSHKLNASAKNLQNF
ITGRRRSGHYDGRTSRKLPNDFLTSVVDLIGAAKSLLAWLDRSPFAAVTDYSVTRNNV
IQLCLELTTIVQQDCTVYETENKILHVCKTLSGVCDHIISLSSDPLVSQSAHLEVIQL
ANIKPSEGLGMYIKSTYDGLHVITGTTENSPADRCKKIHAGDEVIQVNHQTVPLIPRS
PTSSVATPSSTISTPTKRDSSALQDLYIPPPPAEPYIPRDEKGNLPCEDLRGHMVGKP
VHKGSESPNSFLDQEYRKRFNIVEEDTVLYCYEYEKGRSSSQGRRESTPTYGKLRPIS
MPVEYNWVGDYEDPNKMKRDSRRENSLLRYMSNEKIAQEEYMFQRNSKKDTGKKSKKK
GDKSNSPTHYSLLPSLQMDALRQDIMGTPVPETTLYHTFQQSSLQHKSKKKNKGPIAG
KSKRRISCKDLGRGDCEGWLWKKKDAKSYFSQKWKKYWFVLKDASLYWYINEEDEKAE
GFISLPEFKIDRASECRKKYAFKACHPKIKSFYFAAEHLDDMNRWLNRINMLTAGYAE
RERIKQEQDYWSESDKEEADTPSTPKQDSPPPPYDTYPRPPSMSCASPYVEAKHSRLS
STETSQSQSSHEEFRQEVTGSSAVSPIRKTASQRRSWQDLIETPLTSSGLHYLQTLPL
EDSVFSDSAAISPEHRRQSTLPTQKCHLQDHYGPYPLAESERMQVLNGNGGKPRSFTL
PRDSGFNHCCLNAPVSACDPQDDVQPPEVEEEEEEEEEEGEAAGENIGEKS"
BASE COUNT 1056 a 800 c 880 g 768 t
ORIGIN
1 actcccgcgc gtgaggcgag cgggcaagtt ggctgagggc gtgcggcaga ggctgcttcc
61 ctcggcgacg cgacccctca gcaactcaag ctatgaactg aagctcccta gggacggaga
121 ccggagcgga gcggcggagg cagcagcagc agcagcagca gcagcagcag cagcagccgc
181 cgccgccgcc gccttagcgg gaactgagca gacccggcgc ggagccacga ctcctgcacg
241 tttacctccc tgtcgccgtt cctgccggcg gttggctaaa agacgttaca gccgcgagac
301 ccgacacaca aaagccgctt tctccgcgcc gcccgcccag ggaggctgcg gccagcaagg
361 gaccccacct gagagcagct cgggctgctg agttcgtttt gtgtctgagc tctgcgctct
421 gcacggaacc gaccccgtac ccatggctct gataatggaa ccggtgagca aatggtctcc
481 gagtcaagta gtggactgga tgaaaggtct tgatgactgt ttgcagcagt atattaagaa
541 ctttgagagg gagaagatca gtggggacca gctgctgcgc attacacatc aggagctaga
601 agatctgggg gtcagccgca ttggccatca ggaactgatc ttggaagcag ttgaccttct
661 gtgtgcattg aattatggct tggaaacaga aaatctaaaa accctttctc acaagttgaa
721 tgcatctgcc aaaaatctgc agaattttat aacaggaagg agaaggagtg gccattatga
781 tgggaggacc agccgaaaat tgccaaacga ctttctgacc tcagttgtgg atctgattgg
841 agcagccaag agtctgcttg cctggttgga caggtcacca tttgctgctg tgacagacta
901 ttcagttaca agaaataatg tcatacaact ctgcctggag ttaacaacaa ttgtgcaaca
961 ggattgtact gtatatgaaa cagagaataa aattcttcac gtgtgtaaaa ctctttctgg
1021 agtctgtgac cacatcatat ccctgtcgtc agatcctctg gtttcacagt ctgctcacct
1081 ggaagtgatt caactggcaa acattaaacc aagcgaaggg ctgggtatgt atattaaatc
1141 tacatatgat ggcctccatg taattactgg aaccacagaa aattcacctg cagatcggtg
1201 caagaaaatc catgctggcg atgaagtgat tcaagttaat catcagactg tgcctcttat
1261 acctagaagt cccacaagca gcgttgccac gccttccagc accatcagta cacccaccaa
1321 aagagacagt tctgccctcc aggatctcta cattccccct cctcctgcag aaccatatat
1381 tcccagggat gaaaaaggaa accttccttg tgaagacctc agaggacata tggtgggcaa
1441 gccagtgcat aagggatctg aatcaccaaa ttcatttctg gatcaggaat atcgaaagag
1501 atttaatatt gtcgaagaag atactgtctt atattgctat gaatatgaaa aaggaagatc
1561 aagtagtcaa ggaagacgag aaagcacccc aacttatggc aagctacgac ctatatctat
1621 gccagtggaa tataattggg tgggggacta tgaagatcca aataagatga agagagatag
1681 tagaagagaa aactctctac ttcggtatat gagcaatgaa aagattgctc aagaagaata
1741 catgtttcag agaaacagca aaaaggacac agggaagaag tcaaaaaaga agggtgataa
1801 gagtaatagc ccaactcact attcattgct acctagttta caaatggatg cactgagaca
1861 agacatcatg ggcactcctg tgccagagac cacactatac catacatttc agcagtcctc
1921 actgcagcac aaatcaaaga agaaaaacaa aggtcctata gcaggcaaga gcaaaagacg
1981 aatttcttgc aaagatcttg gccgtggtga ctgtgagggc tggctttgga aaaagaaaga
2041 tgcgaagagt tacttttcac agaaatggaa aaaatattgg tttgtcctaa aggatgcatc
2101 cctttattgg tatattaatg aggaggatga aaaagcagaa ggattcatta gcctgcctga
2161 atttaaaatt gatagagcca gtgaatgccg caaaaaatat gcattcaaag cctgtcatcc
2221 taaaatcaaa agcttttatt ttgctgctga acatcttgat gatatgaaca ggtggcttaa
2281 cagaattaat atgctgactg caggatatgc agaaagagag aggattaagc aggaacaaga
2341 ttactggagt gagagtgaca aggaagaagc agatactcca tcaacaccaa aacaagatag
2401 ccctccaccc ccatatgata catacccacg acctccctcg atgagttgcg ccagtcctta
2461 tgtggaagca aaacatagcc gactttcctc cacggagact tctcagtctc agtcttctca
2521 tgaggagttt cgccaggaag taactgggag cagtgcagtg tctcccattc gcaagacagc
2581 cagtcagcgc cgctcctggc aggatttaat tgagacgcca ctgacaagtt caggcttaca
2641 ctatcttcag actctgcccc tggaggattc tgtcttctct gactccgcgg ccatctcccc
2701 agagcacagg cggcagtcta ccctgccaac tcagaaatgc cacctgcagg atcactatgg
2761 gccatacccc ttagctgaga gtgagaggat gcaagtgcta aatggaaatg ggggcaagcc
2821 tcgaagtttt actctgcctc gagatagcgg gttcaaccat tgctgtctga atgctccagt
2881 tagtgcctgt gacccacagg atgacgtgca acccccagag gtggaggaag aggaggagga
2941 ggaggaggag gaaggggagg cagcagggga aaacatagga gaaaaaagct aatacactgc
3001 gagagttggt agaacctctc catgccaaat cggatccact tctgttggca ctcaacccat
3061 tggactcaca gattgataag ctaatgttta gagaatttag atcggagaga gtcggtacgg
3121 cgcagactca acatcaacct cttgcaagca actaaaatgg cctcgtcctt gctgtttata
3181 acagaaaaca gacttgtaaa aagcttagat catcaagtgt tttggattgg gggcctccca
3241 aagggatata agaggggcag gccactctta agaagaatgc gagctttcta cattgggact
3301 agcataagat caaagccaat caagatggag cacagtaaca gaaaactgcg gtttctgtgg
3361 gagaacagaa ggggaaaggg tcttaactgg gaaagggctc tgtgtggtaa cacctcagtt
3421 gtgttctcct gacaccagga aaagagaggg atcagcttca ataactagaa aattctggct
3481 gtttaatgga ctctttggtg gcct
//