LOCUS AK294541 1789 bp mRNA linear HUM 24-JUL-2008
DEFINITION Homo sapiens cDNA FLJ53595 complete cds, highly similar to
Iduronate 2-sulfatase precursor (EC 3.1.6.13).
ACCESSION AK294541
VERSION AK294541.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1789)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..1789
/clone="BRAMY3018824"
/clone_lib="BRAMY3"
/db_xref="H-InvDB:HIT000489165"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/organism="Homo sapiens"
/tissue_type="amygdala"
CDS 399..1781
/codon_start=1
/note="highly similar to Iduronate 2-sulfatase precursor
(EC 3.1.6.13)"
/protein_id="BAG57748.1"
/transl_table=1
/translation="MPLRRRPDTTRLYDFNSYWRVHAGNFSTIPQYFKENGYVTMSVG
KVFHPGISSNHTDDSPYSWSFPPYHPSSEKYENTKTCRGPDGELHANLLCPVDVLDVP
EGTLPDKQSTEQAIQLLEKMKTSASPFFLAVGYHKPHIPFRYPKEFQKLYPLENITLA
PDPEVPDGLPPVAYNPWMDIRQREDVQALNISVPYGPIPVDFQRKIRQSYFASVSYLD
TQVGRLLSALDDLQLANSTIIAFTSDHGWALGEHGEWAKYSNFDVATHVPLIFYVPGR
TASLPEAGEKLFPYLDPFDSASQLMEPGRQSMDLVELVSLFPTLAGLAGLQVPPRCPV
PSFHVELCREGKNLLKHFRFRDLEEDPYLPGNPRELIAYSQYPRPSDIPQWNSDKPSL
KDIKIMGYSIRTIDYRYTVWVGFNPDEFLANFSDIHAGELYFVDSDPLQDHNMYNDSQ
GGDLFQLLMP"
BASE COUNT 378 a 517 c 445 g 449 t
ORIGIN
1 ggcactcgcg ctgcggccag cgcccgggcc tgcgggcccg ggcggcggct gtgttgcgca
61 gtcttcatgg gttcccgacg aggaggtctc tgtggctgcg gcggcggctg ctaactgcgc
121 cacctgctgc agcctgtccc cgccgctctg aagcggccgc gtcgaagccg aaatgccgcc
181 accccggacc ggccgaggcc ttctctggct gggtctggtt ctgagctccg tctgcgtcgc
241 cctcggatcc gaaacgcagg ccaactcgac cacagatgct ctgaacgttc ttctcatcat
301 cgtggatgac ctgcgcccct ccctgggctg ttatggggat aagctggtga ggtccccaaa
361 tattgaccaa ctggcatccc acagcctcct cttccagaat gcctttgcgc aggagacctg
421 acaccacccg cctgtacgac ttcaactcct actggagggt gcacgctgga aacttctcca
481 ccatccccca gtacttcaag gagaatggct atgtgaccat gtcggtggga aaagtctttc
541 accctgggat atcttctaac cataccgatg attctccgta tagctggtct tttccacctt
601 atcatccttc ctctgagaag tatgaaaaca ctaagacatg tcgagggcca gatggagaac
661 tccatgccaa cctgctttgc cctgtggatg tgctggatgt tcccgagggc accttgcctg
721 acaaacagag cactgagcaa gccatacagt tgttggaaaa gatgaaaacg tcagccagtc
781 ctttcttcct ggccgttggg tatcataagc cacacatccc cttcagatac cccaaggaat
841 ttcagaagtt gtatcccttg gagaacatca ccctggcccc cgatcccgag gtccctgatg
901 gcctaccccc tgtggcctac aacccctgga tggacatcag gcaacgggaa gacgtccaag
961 ccttaaacat cagtgtgccg tatggtccaa ttcctgtgga ctttcagcgg aaaatccgcc
1021 agagctactt tgcctctgtg tcatatttgg atacacaggt cggccgcctc ttgagtgctt
1081 tggacgatct tcagctggcc aacagcacca tcattgcatt tacctcggat catgggtggg
1141 ctctaggtga acatggagaa tgggccaaat acagcaattt tgatgttgct acccatgttc
1201 ccctgatatt ctatgttcct ggaaggacgg cttcacttcc ggaggcaggc gagaagcttt
1261 tcccttacct cgaccctttt gattccgcct cacagttgat ggagccaggc aggcaatcca
1321 tggaccttgt ggaacttgtg tctctttttc ccacgctggc tggacttgca ggactgcagg
1381 ttccacctcg ctgccccgtt ccttcatttc acgttgagct gtgcagagaa ggcaagaacc
1441 ttctgaagca ttttcgattc cgtgacttgg aagaggatcc gtacctccct ggtaatcccc
1501 gtgaactgat tgcctatagc cagtatcccc ggccttcaga catccctcag tggaattctg
1561 acaagccgag tttaaaagat ataaagatca tgggctattc catacgcacc atagactata
1621 ggtatactgt gtgggttggc ttcaatcctg atgaatttct agctaacttt tctgacatcc
1681 atgcagggga actgtatttt gtggattctg acccattgca ggatcacaat atgtataatg
1741 attcccaagg tggagatctt ttccagttgt tgatgccttg agttttgcc
//