LOCUS AK294428 2980 bp mRNA linear HUM 24-JUL-2008
DEFINITION Homo sapiens cDNA FLJ56506 complete cds, highly similar to
Hexokinase-1 (EC 2.7.1.1).
ACCESSION AK294428
VERSION AK294428.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2980)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..2980
/clone="BRAMY3002801"
/clone_lib="BRAMY3"
/db_xref="H-InvDB:HIT000489052"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/organism="Homo sapiens"
/tissue_type="amygdala"
CDS 99..2846
/codon_start=1
/note="highly similar to Hexokinase-1 (EC 2.7.1.1)"
/protein_id="BAG57673.1"
/transl_table=1
/translation="MIAAQLLAYYFTELKDDQVKKIDKYLYAMRLSDETLIDIMTRFR
KEMKNGLSRDFNPTATVKMLPTFVRSIPDGSEKGDFIALDLGGSSFRILRVQVNHEKN
QNVHMESEVYDTPENIVHGSGSQLFDHVAECLGDFMEKRKIKDKKLPVGFTFSFPCQQ
SKIDEAILITWTKRFKASGVEGADVVKLLNKAIKKRGDYDANIVAVVNDTVGTMMTCG
YDDQHCEVGLIIGTGTNACYMEELRHIDLVEGDEGRMCINTEWGAFGDDGSLEDIRTE
FDREIDRGSLNPGKQLFEKMVSGMYLGELVRLILVKMAKEGLLFEGRITPELLTRGKF
NTSDVSAIEKNKEGLHNAKEILTRLGVEPSDDDCVSVQHVCTIVSFRSANLVAATLGA
ILNRLRDNKGTPRLRTTVGVDGSLYKTHPQYSRRFHKTLRRLVPDSDVRFLLSESGSG
KGAAMVTAVAYRLAEQHRQIEETLAHFHLTKDMLLEVKKRMRAEMELGLRKQTHNNAV
VKMLPSFVRRTPDGTENGDFLALDLGGTNFRVLLVKIRSGKKRTVEMHNKIYAIPIEI
MQGTGEELFDHIVSCISDFLDYMGIKGPRMPLGFTFSFPCQQTSLDAGILITWTKGFK
ATDCVGHDVVTLLRDAIKRREEFDLDVVAVVNDTVGTMMTCAYEEPTCEVGLIVGTGS
NACYMEEMKNVEMVEGDQGQMCINMEWGAFGDNGCLDDIRTHYDRLVDEYSLNAGKQR
YEKMISGMYLGEIVRNILIDFTKKGFLFRGQISETLKTRGIFETKFLSQIESDRLALL
QVRAILQQLGLNSTCDDSILVKTVCGVVSRRAAQLCGAGMAAVVDKIRENRGLDRLNV
TVGVDGTLYKLHPHFSRIMHQTVKELSPKCNVSFLLSEDGSGKGAALITAVGVRLRTE
A"
BASE COUNT 732 a 736 c 883 g 629 t
ORIGIN
1 gaggagccgc cgagcagccg ccggaggacc acggctcgcc agggctgcgg aggaccgacc
61 gtccccacgc ctgccgcccc gcgaccccga ccgccagcat gatcgccgcg cagctcctgg
121 cctattactt cacggagctg aaggatgacc aggtcaaaaa gattgacaag tatctctatg
181 ccatgcggct ctccgatgaa actctcatag atatcatgac tcgcttcagg aaggagatga
241 agaatggcct ctcccgggat tttaatccaa cagccacagt caagatgttg ccaacattcg
301 taaggtccat tcctgatggc tctgaaaagg gagatttcat tgccctggat cttggtgggt
361 cttcctttcg aattctgcgg gtgcaagtga atcatgagaa aaaccagaat gttcacatgg
421 agtccgaggt ttatgacacc ccagagaaca tcgtgcacgg cagtggaagc cagctttttg
481 atcatgttgc tgagtgcctg ggagatttca tggagaaaag gaagatcaag gacaagaagt
541 tacctgtggg attcacgttt tcttttcctt gccaacaatc caaaatagat gaggccatcc
601 tgatcacctg gacaaagcga tttaaagcga gcggagtgga aggagcagat gtggtcaaac
661 tgcttaacaa agccatcaaa aagcgagggg actatgatgc caacatcgta gctgtggtga
721 atgacacagt gggcaccatg atgacctgtg gctatgacga ccagcactgt gaagtcggcc
781 tgatcatcgg cactggcacc aatgcttgct acatggagga actgaggcac attgatctgg
841 tggaaggaga cgaggggagg atgtgtatca atacagaatg gggagccttt ggagacgatg
901 gatcattaga agacatccgg acagagtttg acagggagat agaccgggga tccctcaacc
961 ctggaaaaca gctgtttgag aagatggtca gtggcatgta cttgggagag ctggttcgac
1021 tgatcctagt caagatggcc aaggagggcc tcttatttga agggcggatc accccggagc
1081 tgctcacccg agggaagttt aacaccagtg atgtgtcagc catcgaaaag aataaggaag
1141 gcctccacaa tgccaaagaa atcctgaccc gcctgggagt ggagccgtcc gatgatgact
1201 gtgtctcagt ccagcacgtt tgcaccattg tctcatttcg ctcagccaac ttggtggctg
1261 ccacactggg cgccatcttg aaccgcctgc gtgataacaa gggcacaccc aggctgcgga
1321 ccacggttgg tgtcgacgga tctctttaca agacgcaccc acagtattcc cggcgtttcc
1381 acaagactct aaggcgcttg gtgccagact ccgatgtgcg cttcctcctc tcggagagtg
1441 gcagcggcaa gggggctgcc atggtgacgg cggtggccta ccgcttggcc gagcagcacc
1501 ggcagataga ggagaccctg gctcatttcc acctcaccaa ggacatgctg ctggaggtga
1561 agaagaggat gcgggccgag atggagctgg ggctgaggaa gcagacgcac aacaatgccg
1621 tggttaagat gctgccctcc ttcgtccgga gaactcccga cgggaccgag aatggtgact
1681 tcttggccct ggatcttgga ggaaccaatt tccgtgtgct gctggtgaaa atccgtagtg
1741 ggaaaaagag aacggtggaa atgcacaaca agatctacgc cattcctatt gaaatcatgc
1801 agggcactgg ggaagagctg tttgatcaca ttgtctcctg catctctgac ttcttggact
1861 acatggggat caaaggcccc aggatgcctc tgggcttcac gttctcattt ccctgccagc
1921 agacgagtct ggacgcggga atcttgatca cgtggacaaa gggttttaag gcaacagact
1981 gcgtgggcca cgatgtagtc accttactaa gggatgcgat aaaaaggaga gaggaatttg
2041 acctggacgt ggtggctgtg gtcaacgaca cagtgggcac catgatgacc tgtgcttatg
2101 aggagcccac ctgtgaggtt ggactcattg ttgggaccgg cagcaatgcc tgctacatgg
2161 aggagatgaa gaacgtggag atggtggagg gggaccaggg gcagatgtgc atcaacatgg
2221 agtggggggc ctttggggac aacgggtgtc tggatgatat caggacacac tacgacagac
2281 tggtggacga atattcccta aatgctggga aacaaaggta tgagaagatg atcagtggta
2341 tgtacctggg tgaaatcgtc cgcaacatct taatcgactt caccaagaag ggattcctct
2401 tccgagggca gatctctgag acgctgaaga cccggggcat ctttgagacc aagtttctct
2461 ctcagatcga gagtgaccga ttagcactgc tccaggtccg ggctatcctc caacagctag
2521 gtctgaatag cacctgcgat gacagtatcc tcgtcaagac agtgtgcggg gtggtgtcca
2581 ggagggccgc acagctgtgt ggcgcaggca tggctgcggt tgtggataag atccgcgaga
2641 acagaggact ggaccgtctg aatgtgactg tgggagtgga cgggacactc tacaagcttc
2701 atccacactt ctccagaatc atgcaccaga cggtgaagga actgtcacca aaatgtaacg
2761 tgtccttcct cctgtctgag gatggcagcg gcaagggggc cgccctcatc acggccgtgg
2821 gcgtgcggtt acgcacagag gcatagcatt agctgcttcc tcccctcctg gcacccactg
2881 tggcctggca tcgcatcgtg gtgtgtcaat gccacaaaat cgtgtgtccg tggaaccagt
2941 cctagccgcg tgtgacagtc ttgcattctg tttgtctcgt
//