LOCUS AK294315 2004 bp mRNA linear HUM 24-JUL-2008
DEFINITION Homo sapiens cDNA FLJ56065 complete cds, highly similar to
Pyruvate kinase isozyme M1 (EC 2.7.1.40).
ACCESSION AK294315
VERSION AK294315.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2004)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..2004
/clone="BRAMY2031229"
/clone_lib="BRAMY2"
/db_xref="H-InvDB:HIT000488939"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/organism="Homo sapiens"
/tissue_type="amygdala"
CDS 127..1944
/codon_start=1
/note="highly similar to Pyruvate kinase isozyme M1 (EC
2.7.1.40)"
/protein_id="BAG57589.1"
/transl_table=1
/translation="MQWSSERGERLLTPGACSSEVPSAVPSRSGGSPGHTVFSSERSL
LVRPRSHPEPKGEHYVTGSPTPENQRTSAAMSKPHSEAGTAFIQTQQLHAAMADTFLE
HMCRLDIDSPPITARNTGIICTIGPASRSVETLKEMIKSGMNVARLNFSHGTHEYHAE
TIKNVRTATESFASDPILYRPVAVALDTKGPEIRTGLIKGSGTAEVELKKGATLKITL
DNAYMEKCDENILWLDYKNICKVVEVGSKIYVDDGLISLQVKQKGADFLVTEVENGGS
LGSKKGVNLPGAAVDLPAVSEKDIQDLKFGVEQDVDMVFASFIRKASDVHEVRKVLGE
KGKNIKIISKIENHEGVRRFDEILEASDGIMVARGDLGIEIPAEKVFLAQKMMIGRCN
RAGKPVICATQMLESMIKKPRPTRAEGSDVANAVLDGADCIMLSGETAKGDYPLEAVR
MQHLIAREAEAAMFHRKLFEELVRASSHSTDLMEAMAMGSVEASYKCLAAALIVLTES
GRSAHQVARYRPRAPIIAVTRNPQTARQAHLYRGIFPVLCKDPVQEAWAEDVDLRVNF
AMNVGKARGFFKKGDVVIVLTGWRPGSGFTNTMRVVPVP"
BASE COUNT 452 a 547 c 602 g 403 t
ORIGIN
1 gagcggtgca ggaggctacg ccatcagtcc ccaccaaggg ccagtcgccc ggctagtgcg
61 gaatcccggc gcgccggccg gccccgggca cgcaggcagg gcggcgcagg atccagggcg
121 tctgggatgc agtggagctc agagagagga gaacggctcc tcacgcctgg ggcctgctct
181 tcagaagtcc ccagcgccgt tccttccaga tcaggcggct ctccagggca caccgtattc
241 agctctgagc ggtctttgct agtgaggcca aggagccacc ctgagccaaa aggggagcat
301 tatgtcaccg gaagcccaac cccagagaac caaaggacct cagcagccat gtcgaagccc
361 catagtgaag ccgggactgc cttcattcag acccagcagc tgcacgcagc catggctgac
421 acattcctgg agcacatgtg ccgcctggac attgattcac cacccatcac agcccggaac
481 actggcatca tctgtaccat tggcccagct tcccgatcag tggagacgtt gaaggagatg
541 attaagtctg gaatgaatgt ggctcgtctg aacttctctc atggaactca tgagtaccat
601 gcggagacca tcaagaatgt gcgcacagcc acggaaagct ttgcttctga ccccatcctc
661 taccggcccg ttgctgtggc tctagacact aaaggacctg agatccgaac tgggctcatc
721 aagggcagcg gcactgcaga ggtggagctg aagaagggag ccactctcaa aatcacgctg
781 gataacgcct acatggaaaa gtgtgacgag aacatcctgt ggctggacta caagaacatc
841 tgcaaggtgg tggaagtggg cagcaagatc tacgtggatg atgggcttat ttctctccag
901 gtgaagcaga aaggtgccga cttcctggtg acggaggtgg aaaatggtgg ctccttgggc
961 agcaagaagg gtgtgaacct tcctggggct gctgtggact tgcctgctgt gtcggagaag
1021 gacatccagg atctgaagtt tggggtcgag caggatgttg atatggtgtt tgcgtcattc
1081 atccgcaagg catctgatgt ccatgaagtt aggaaggtcc tgggagagaa gggaaagaac
1141 atcaagatta tcagcaaaat cgagaatcat gagggggttc ggaggtttga tgaaatcctg
1201 gaggccagtg atgggatcat ggtggctcgt ggtgatctag gcattgagat tcctgcagag
1261 aaggtcttcc ttgctcagaa gatgatgatt ggacggtgca accgagctgg gaagcctgtc
1321 atctgtgcta ctcagatgct ggagagcatg atcaagaagc cccgccccac tcgggctgaa
1381 ggcagtgatg tggccaatgc agtcctggat ggagccgact gcatcatgct gtctggagaa
1441 acagccaaag gggactatcc tctggaggct gtgcgcatgc agcacctgat agctcgtgag
1501 gctgaggcag ccatgttcca ccgcaagctg tttgaagaac ttgtgcgagc ctcaagtcac
1561 tccacagacc tcatggaagc catggccatg ggcagcgtgg aggcttctta taagtgttta
1621 gcagcagctt tgatagttct gacggagtct ggcaggtctg ctcaccaggt ggccagatac
1681 cgcccacgtg cccccatcat tgctgtgacc cggaatcccc agacagctcg tcaggcccac
1741 ctgtaccgtg gcatcttccc tgtgctgtgc aaggacccag tccaggaggc ctgggctgag
1801 gacgtggacc tccgggtgaa ctttgccatg aatgttggca aggcccgagg cttcttcaag
1861 aagggagatg tggtcattgt gctgaccgga tggcgccctg gctccggctt caccaacacc
1921 atgcgtgttg ttcctgtgcc gtgatggacc ccagagcccc tcctccagcc cctgtcccac
1981 ccccttcccc cagcccatcc atta
//