LOCUS AK294107 1637 bp mRNA linear HUM 24-JUL-2008
DEFINITION Homo sapiens cDNA FLJ60473 complete cds, moderately similar to
Homo sapiens NEDD8-conjugating enzyme (NCE2), mRNA.
ACCESSION AK294107
VERSION AK294107.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1637)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..1637
/clone="BRALZ2009231"
/clone_lib="BRALZ2"
/db_xref="H-InvDB:HIT000488731"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/organism="Homo sapiens"
/tissue_type="alzheimer cortex"
CDS 85..393
/codon_start=1
/note="moderately similar to Homo sapiens
NEDD8-conjugating enzyme (NCE2), mRNA"
/protein_id="BAG57442.1"
/transl_table=1
/translation="MLTLASKLKRDDGLKGSRTAATASDSTRRVSVRDKLLVKEVAEL
EANLPCTCKVHFPDPNKLHCFQLTVTPASQSEMPDQDLAPQHHRDRGNMSEFIERTFN
"
BASE COUNT 446 a 343 c 384 g 464 t
ORIGIN
1 gcagcagccg cccggaccgg gcatggtgtt gggcgccggg cccgcctcgc ctgtctcggg
61 gagcccaggg taaaggcagc agtaatgcta acgctagcaa gtaaactgaa gcgtgacgat
121 ggtctcaaag ggtcccggac ggcagccaca gcgtccgact cgactcggag ggtttctgtg
181 agagacaaat tgcttgttaa agaggttgca gaacttgaag ctaatttacc ttgtacatgt
241 aaagtgcatt ttcctgatcc aaacaagctt cattgttttc agctaacagt aaccccagcc
301 tcccaaagtg aaatgcctga ccaagatctg gcaccccaac atcacagaga caggggaaat
361 atgtctgagt ttattgagag aacattcaat tgatggcact ggctgggctc ccacaagaac
421 attaaaggat gtcgtttggg gattaaactc tttgtttact gatcttttga attttgatga
481 tccactgaat attgaagctg cagaacatca tttgcgggac aaggaggact tccggaataa
541 agtggatgac tacatcaaac gttatgccag atgataaaag gggacgattg caggcccatg
601 gactgtgtta cagtttgtct ctaacatgaa acagcaagag gtagccccct ctcccgtcct
661 catgctccct ctcagtcccc tggattgccc cagtcctgtg accatgttgc cctgaagaag
721 accatcttca tgactgctca ttgtagatgg agaattcaac ataaatacag caagaaaatg
781 tgtttgggct tctgaagagt tgtctgctta ccttaacatg tttacttttt tgaacttgta
841 ctgtataggc tgttggtgaa attcttaaga agttgtaatg aactcaaaat tgaggccaga
901 gcttgctttc ccttttccca aacaaaattg gttttctgca caagcgatgc taatgatgtg
961 ttcagtgtaa ctcgcagatt ggcaataaga tacccgctac aaactgtgat tggatgcaaa
1021 atctcttagc ttctttcacg aatgttggcc ctgcctagat gttgtgaagc ctcccagaat
1081 gcatagagtc attcactgta gatctcttat tgaaatgcgt attttattta atgtaagtat
1141 attttggaac agatttgtaa tttgtacaat tcaatgcttt aattattttt tctattctca
1201 tttagtttgt attttcattg tatagagcag acagaaagat gttgggtcaa gcaactattg
1261 aagagaaata caaagaaaat atgaaaggca cattattcat tttgtccaaa tgcaatgaga
1321 atctcactct taaaaatcag ctcttgcttt cgggtccgga tgtggtgagc acattttgga
1381 gccctttgaa gctagatttg gatgatcgaa acaaaaaggc agggagccca ttctaacatg
1441 ctgcccagag gaaactggct ggagcctgga ccagctgggg ttgatgcttt tgcagtggtc
1501 atgtgattgt gacctggtag ctacttatca gagagccaga ccctgctgtc ctgggagaca
1561 ggagcgatgc ctcaggaatc agcccaatgt ctgatgtcac tgagactgta cctgtggcct
1621 tcttctgagt ttgctat
//