LOCUS AK294040 2276 bp mRNA linear HUM 24-JUL-2008
DEFINITION Homo sapiens cDNA FLJ51812 complete cds, highly similar to
Galactocerebrosidase precursor (EC 3.2.1.46).
ACCESSION AK294040
VERSION AK294040.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2276)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..2276
/clone="BRACE3050086"
/clone_lib="BRACE3"
/db_xref="H-InvDB:HIT000488664"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/organism="Homo sapiens"
/tissue_type="cerebellum"
CDS 586..2010
/codon_start=1
/note="highly similar to Galactocerebrosidase precursor
(EC 3.2.1.46)"
/protein_id="BAG57392.1"
/transl_table=1
/translation="MLNYQGLQRVKIIASDNLWESISASMLLDAELFKVVNVIGAHYP
GTHSAKDAKLTGKKLWSSEDFSTLNSDMGAGCWGRILNQNYINGYMTSTIAWNLVASY
YEQLPYGRCGLMTAQEPWSGHYVVESPVWVSAHTTQFTQPGWYYLKTVGHLEKGGSYV
ALTDGLGNLTIIIETMSHKHSKCIRPFLPYFNVSQQFATFVLKGSFSEIPELQVWYTK
LGKTSERFLFKQLDSLWLLDSDGSFTLSLHEDELFTLTTLTTGRKGSYPLPPKSQPFP
STYKDDFNVDYPFFSEAPNFADQTGVFEYFTNIEDPGEHHFTLRQVLNQRPITWAADA
SNTISIIGDYNWTNLTIKCDVYIETPDTGGVFIAGRVNKGGILIRSARGIFFWIFANG
SYRVTGDLAGWIIYALGRVEVTAKKWYTLTLTIKGHFASGMLNDKSLWTDIPVNFPKN
GWAAIGTHSFEFAQFDNFLVEATR"
BASE COUNT 616 a 480 c 536 g 644 t
ORIGIN
1 atgtgaccca cacaatggct gagtggctac tctcggcttc ctggcaacgc cgagccaaag
61 ctatgactgc ggccccgggt tcggcgggac gcgccgcggt gcccttgctg ctgtgtgcgc
121 tgctggcgcc cggcggcgcg tacgtgctcg acgactccga cgggctgggc cgggagttcg
181 acggcatcgg cgcggtcagc ggcggcgggg caacctcccg acttctagta aattacccag
241 agccctatcg ttctcagata ttggattatc tctttaagcc gaattttggt gcctctttgc
301 atattttaaa agtggaaata ggtggtgatg ggcagacaac agacggcact gagccctccc
361 acatgcatta tgcactagat gagaattatt tccgaggata cgagtggtgg ttgatgaaag
421 aagctaagaa gaggaatccc aatattacac tcattgggtt gccatggtca ttccctggat
481 ggctggattg tgggcgccaa gcgttaccat gatttggaca ttgattatat tggaatttgg
541 aatgagaggt catataatgc caattatatt aagatattaa gaaaaatgct gaattatcaa
601 ggtctccagc gagtgaaaat catagcaagt gataatctct gggagtccat ctctgcatcc
661 atgctccttg atgccgaact cttcaaggtg gttaatgtta taggggctca ttatcctgga
721 acccattcag caaaagatgc aaagttgact gggaagaagc tttggtcttc tgaagacttt
781 agcactttaa atagtgacat gggtgcaggc tgctggggtc gcattttaaa tcagaattat
841 atcaatggct atatgacttc cacaatcgca tggaatttag tggctagtta ctatgaacag
901 ttgccttatg ggagatgcgg gttgatgacg gcccaggagc catggagtgg gcactacgtg
961 gtagaatctc ctgtctgggt atcagctcat accactcagt ttactcaacc tggctggtat
1021 tacctgaaga cagttggcca tttagagaaa ggaggaagct acgtagctct gactgatggc
1081 ttagggaacc tcaccatcat cattgaaacc atgagtcata aacattctaa gtgcatacgg
1141 ccatttcttc cttatttcaa tgtgtcacaa caatttgcca cctttgttct taagggatct
1201 tttagtgaaa taccagagct acaggtatgg tataccaaac ttggaaaaac atccgaaaga
1261 tttcttttta agcagctgga ttctctatgg ctccttgaca gcgatggcag tttcacactg
1321 agcctgcatg aagatgagct gttcacactc accactctca ccactggtcg caaaggcagc
1381 tacccgcttc ctccaaaatc ccagcccttc ccaagtacct ataaggatga tttcaatgtt
1441 gattacccat tttttagtga agctccaaac tttgctgatc aaactggtgt atttgaatat
1501 tttacaaata ttgaagaccc tggcgagcat cacttcacgc tacgccaagt tctcaaccag
1561 agacccatta cgtgggctgc cgatgcatcc aacacaatca gtattatagg agactacaac
1621 tggaccaatc tgactataaa gtgtgatgtt tacatagaga cccctgacac aggaggtgtg
1681 ttcattgcag gaagagtaaa taaaggtggt attttgatta gaagtgccag aggaattttc
1741 ttctggattt ttgcaaatgg atcttacagg gttacaggtg atttagctgg atggattata
1801 tatgctttag gacgtgttga agttacagca aaaaaatggt atacactcac gttaactatt
1861 aagggtcatt tcgcctctgg catgctgaat gacaagtctc tgtggacaga catccctgtg
1921 aattttccaa agaatggctg ggctgcaatt ggaactcact cctttgaatt tgcacagttt
1981 gacaactttc ttgtggaagc cacacgctaa tacttaacag ggcatcatag aatactctgg
2041 attttcttcc cttctttttg gttttggttc agagccaatt cttgtttcat tggaacagta
2101 tatgaggctt ttgagactaa aaataatgaa gagtaaaagg ggagagaaat ttatttttaa
2161 tttaccctgt ggaagatttt attagaatta attccaaggg gaaaactggt gaatctttaa
2221 cattacctgg tgtgttccct aacattcaaa ctgtgcattg gccataccct taggag
//