LOCUS AK293956 3620 bp mRNA linear HUM 24-JUL-2008
DEFINITION Homo sapiens cDNA FLJ56476 complete cds, highly similar to Solute
carrier family 12 member 4.
ACCESSION AK293956
VERSION AK293956.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3620)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..3620
/clone="BRACE3033869"
/clone_lib="BRACE3"
/db_xref="H-InvDB:HIT000488580"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/organism="Homo sapiens"
/tissue_type="cerebellum"
CDS 81..3194
/codon_start=1
/note="highly similar to Solute carrier family 12 member
4"
/protein_id="BAG57330.1"
/transl_table=1
/translation="MTTSRGSVGWTTGSAPSWMTRTEELDIRPKVSSLLGKLVSYTNL
TQGAKEHEEAESGEGTRRRAAEAPSMGTLMGVYLPCLQNIFGVILFLRLTWMVGTAGV
LQALLIVLICCCCTLLTAISMSAIATNGVVPAGGSYFMISRSLGPEFGGAVGLCFYLG
TTFAAAMYILGAIEILLTYIAPPAAIFYPSGAHDTSNATLNNMRVYGTIFLTFMTLVV
FVGVKYVNKFASLFLACVIISILSIYAGGIKSIFDPPVFPVCMLGNRTLSRDQFDICA
KTAVVDNETVATQLWSFFCHSPNLTTDSCDPYFMLNNVTEIPGIPRAAAGVLQENLWS
AYLEKGDIVEKHGLPSADAPSLKESLPLYVVADIATSFTVLVGIFFPSVTGIMAGSNR
SGDLRDAQKSIPVGTILAIITTSLVYFSSVVLFGACIEGVVLRDKYGDGVSRNLVVGT
LAWPSPWVIVIGSFFSTCGAGLQSLTGAPRLLQAIAKDNIIPFLRVFGHGKVNGEPTW
ALLLTALIAELGILIASLDMVAPILSMFFLMCYLFVNLACAVQTLLRTPNWRPRFKYY
HWALSFLGMSLCLALMFVSSWYYALVAMLIAGMIYKYIEYQGAEKEWGDGIRGLSLSA
ARYALLRLEEGPPHTKNWRPQLLVLLKLDEDLHVKYPRLLTFASQLKAGKGLTIVGSV
IQGSFLESYGEAQAAEQTIKNMMEIEKVKGFCQVVVASKVREGLAHLIQSCGLGGMRH
NSVVLGWPYGWRQSEDPRAWKTFIDTVRCTTAAHLALLVPKNIAFYPSNHERYLEGHI
DVWWIVHDGGMLMLLPFLLRQHKVWRKCRMRIFTVAQMDDNSIQMKKDLAVFLYHLRL
EAEVEVVEMHNSDISAYTYERTLMMEQRSQMLRQMRLTKTEREREAQLVKDRHSALRL
ESLYSDEEDESAVGADKIQMTWTRDKYMTETWDPSHAPDNFRELVHIKPDQSNARRMH
TAVKLNEVIVTRSHDARLVLLNMPGPPRNSEGDEDYMEFLEVLTEGLERVLLVRGGGR
EVITIYS"
BASE COUNT 688 a 1063 c 1091 g 778 t
ORIGIN
1 acggcgggtg cggcggggcc gagcccgcac gatgcctcac ttcaccgtgg tgccagtgga
61 cgggccgagg cgcggcgact atgacaacct cgaggggctc agttgggtgg actacgggga
121 gcgcgccgag ctggatgact cggacggaag agctggacat ccgcccaaag gtatcgtctc
181 ttctgggaaa gctcgtcagc tacaccaacc tcacccaggg cgccaaagag catgaggagg
241 ccgagagtgg ggagggcacc cgccggaggg cagccgaggc acccagcatg ggcaccctca
301 tgggggtgta cctgccctgc ctgcagaata tctttggggt tatcctcttc ctgcggctga
361 cctggatggt gggcacagca ggtgtgctac aggccctcct catcgtgctt atctgctgct
421 gttgtaccct gctgacggcc atctccatga gtgccatcgc caccaacggt gtggttccag
481 ctgggggctc ctatttcatg atctctcgtt cactggggcc agaatttgga ggtgctgtgg
541 gcctgtgctt ctacctggga acaacattcg cagcagccat gtacatcctg ggggccatcg
601 agatcttgct gacctacatt gccccaccag ctgccatttt ttacccatcg ggtgctcatg
661 acacgtcgaa tgccactttg aacaatatgc gtgtgtatgg gaccattttc ctgaccttca
721 tgaccctggt ggtgtttgtg ggggtcaagt atgtgaacaa atttgcctcg ctcttcctgg
781 cctgtgtgat catctccatc ctctccatct atgctggggg cataaagtct atatttgacc
841 ctcccgtgtt tccggtatgc atgctgggca acaggaccct gtcccgggac cagtttgaca
901 tctgtgccaa gacagctgta gtggacaatg agacagtggc cacccagcta tggagtttct
961 tctgccacag ccccaacctt acgaccgact cctgtgaccc ctacttcatg ctcaacaatg
1021 tgaccgagat ccctggcatc cccagggcag ctgctggtgt gctccaggaa aacctgtgga
1081 gcgcctacct ggagaagggt gacatcgtgg agaagcatgg gctgccctcc gcagatgccc
1141 cgagcctgaa ggagagcctg cctctgtacg tggtcgctga catcgccaca tccttcaccg
1201 tgctggtcgg catcttcttc ccttctgtaa caggcatcat ggctggctca aaccgctctg
1261 gggaccttcg tgacgcccag aagtctatcc ctgtggggac cattctggcc atcattacaa
1321 cttccctcgt gtacttcagc agtgtggttc tctttggtgc ctgcattgag ggtgtggttc
1381 tccgggacaa gtatggcgat ggtgtcagca ggaacttggt ggtgggcaca ctggcctggc
1441 cttcaccctg ggtcatcgtc atcggctcct tcttttcaac gtgtggcgct ggcctccaga
1501 gcctcacagg ggcaccacgc ctattgcagg ccattgccaa ggacaacatc atccccttcc
1561 tccgggtgtt tggccacggg aaggtgaatg gtgaacccac atgggcactc ctcctgacgg
1621 cactcatcgc cgagctgggc atcctcatcg cctccctcga catggtggcc cccatcttat
1681 ccatgttctt tctgatgtgc tacctgttcg tgaacctcgc ctgtgcggtg cagacactcc
1741 tgaggacccc caactggcgg ccccggttca agtactatca ctgggcgctg tccttcctgg
1801 gcatgagtct ctgcctggcc cttatgtttg tctcctcctg gtactatgcc ctggtggcca
1861 tgctcatcgc cggcatgatc tacaaataca tcgagtacca aggggctgag aaggagtggg
1921 gtgacgggat ccgaggcctg tccctgagcg ctgcccgcta cgcgctgttg cggctggagg
1981 aggggcctcc tcacaccaag aactggcggc cgcagctgct ggtgctgctg aagctggacg
2041 aggacctcca cgtgaagtac ccgcggctcc tcaccttcgc ctcccagctc aaggctggca
2101 agggcctgac cattgttggt tctgtcatcc aggggagctt cttggagagc tatggcgagg
2161 ctcaggccgc cgagcagacc atcaagaaca tgatggaaat tgagaaggtg aagggcttct
2221 gccaggtggt ggtggccagc aaggtgcggg aggggctggc ccacctcatc cagtcctgtg
2281 gcctgggagg catgcggcat aactccgtgg tgctgggctg gccctacggc tggcgacaga
2341 gcgaggaccc ccgtgcctgg aagaccttca ttgacaccgt gcgctgcact acggctgccc
2401 acctggccct gctcgtgccc aagaacatcg ccttctaccc cagcaaccac gagcgctacc
2461 tggagggcca catagacgtg tggtggatcg tgcacgatgg tggcatgctc atgcttctgc
2521 ccttcctgct gcgccagcat aaggtctgga ggaagtgccg gatgcgcatc ttcacagtgg
2581 cccagatgga tgacaacagc atccagatga agaaggacct ggctgtcttt ctgtaccatc
2641 tgcgccttga ggccgaggtg gaggtggtgg agatgcataa cagtgacatc tctgcataca
2701 cctacgagcg gacgctgatg atggagcagc ggtcgcagat gctgcggcag atgagactga
2761 ccaagactga gcgggagcga gaagcccagc tggtcaagga tcggcactcg gccctgcggc
2821 tggagagcct gtactcggac gaggaagatg agtctgcagt gggggctgac aagatccaga
2881 tgacgtggac cagggacaag tacatgactg agacctggga ccccagccat gcccctgaca
2941 atttccggga gctggtgcac attaagccgg accaatccaa tgcgcggcgc atgcacactg
3001 ctgtgaagct caatgaagtc attgtcacgc gctcccacga cgcccgcctg gttctcctaa
3061 acatgcctgg cccacccagg aacagtgagg gcgacgagga ctacatggag ttcctcgagg
3121 tgctgaccga gggccttgag cgggtgctgt tggtgcgcgg tggtggccgt gaagtcatca
3181 ccatctactc ctgagcccag tgtcatcttg tggcctggag tcgaggtctt ggccaggaca
3241 taacaagctg tggtctgggg taacagcctc ttcccagcac ccacctgcca gccctgcttg
3301 cctggccctg tcctggaccc agctttgcta ggtctccttg gaaaccaggc ctgggcctca
3361 aaatggagat ggatcccagg tcttgtggga ccctgggatg tttggggact ttactatcta
3421 gcaccccagt aggcctgtcc tggccagaga agactggtag gggccgagtg gggtttgaag
3481 gcagccggcc cggcccagcc caggagcgct atttattgca tatttattgt ttggatgtca
3541 ccatcagaga cgaagggaag ggtagccagg gagggagtcc agcccagctg cctgcaggaa
3601 gatctggctc agtctactat
//