LOCUS AK293864 2197 bp mRNA linear HUM 21-JAN-2009
DEFINITION Homo sapiens cDNA FLJ58753 complete cds, highly similar to Rho
guanine nucleotide exchange factor 3.
ACCESSION AK293864
VERSION AK293864.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2197)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..2197
/clone="BRACE3020875"
/clone_lib="BRACE3"
/db_xref="H-InvDB:HIT000488488"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/organism="Homo sapiens"
/tissue_type="cerebellum"
CDS 455..1948
/codon_start=1
/note="highly similar to Rho guanine nucleotide exchange
factor 3"
/protein_id="BAH11616.1"
/transl_table=1
/translation="MIEEPSNKRVKPLSRVTSLANLIPPVKATPLKRFSQTLQRSISF
RSESRPDILAPRPWSRNAAPSSTKRRDSKLWSETFDVCVNQMLTSKEIKRQEAIFELS
QGEEDLIEDLKLAKKAYHDPMLKLSIMTEQELNQIFGTLDSLIPLHEELLSQLRDVRK
PDGSTEHVGPILVGWLPCLSSYDSYCSNQVAAKALLDHKKQDHRVQDFLQRCLESPFS
RKLDLWNFLDIPRSRLVKYPLLLREILRHTPNDNPDQQHLEEAINIIQGIVAEINTKT
GESECRYYKERLLYLEEGQKDSLIDSSRVVCCHGELKNNRGVKLHVFLFQEVLVITRA
VTHNEQLCYQLYRQPIPVKDLLLEDLQDGEVRLGGSLRGAFSNNERIKNFFRVSFKNG
SQSQTHSLQANDTFNKQQWLNCIRQAKETVLCAAGQAGVLDSEGSFLNPTTGSRELQG
ETKLEQMDQSDSESDCSMDTSEVSLDCERMEQTDSSCGNSRHGESNV"
BASE COUNT 614 a 524 c 522 g 537 t
ORIGIN
1 aactgagcct tcctagtgtt ctgagagttt tctgctgtct gttcagctta gcacgctaag
61 aagctggaga ggtacccagc agcctggctg cattctccgt cttggaaaag tatttaactg
121 ctttgagaaa tcatggaatc gtttctggcc acattgtgag attgtattga attttctttt
181 taagttttta aatttttata tgcaaatgct ggaaagcttg gagacagtct atttcaaaga
241 aggctgttga tttctgagac tggcaggggc ataaaatgac atgagagtgt ctcagcagga
301 ttccaaattc acgaaagatt tttccaggca cctactatgt tcagaacggg gatgcagaaa
361 tatcctggag aatctgaagt tctgccttaa ccctcgaaac tttgaaagca atggctgaaa
421 aggaaagatg aatacctaga ggctccctct ggaaatgatt gaggagccta gtaataaacg
481 ggtcaaaccc ctttcccgag tcacgtcgct agcaaacctc atcccgcccg tgaaggccac
541 gccattaaag cgcttcagtc aaaccctgca gcgctccatt agcttccgca gtgagagccg
601 ccctgacatc ctcgcccccc gaccctggtc cagaaatgcc gccccctcga gcacgaaacg
661 gagagatagc aagctgtgga gtgagacctt cgatgtgtgc gtcaatcaga tgcttacatc
721 caaggaaatc aaacgtcagg aggcgatctt tgagctttcc caaggagaag aagacttgat
781 agaagacttg aaattagcaa aaaaggccta tcatgacccc atgctgaaac tctccataat
841 gacagaacaa gagttgaatc aaatttttgg aacactggac tctctaattc ctctacatga
901 agagctcctt agtcagcttc gagatgttag gaagcctgat ggctcgactg aacatgttgg
961 tcccatcctc gtgggctggc tcccttgcct cagctcctat gatagctact gcagcaatca
1021 agtagccgcc aaagctctgc tggaccacaa aaagcaagat caccgagtcc aggatttcct
1081 acagcgatgt ttagaatccc cctttagccg caaactagat ctctggaatt tcctcgatat
1141 tccaagaagc cgcctggtaa aataccctct gcttctccga gaaatcttga ggcacacacc
1201 aaatgataat ccagatcagc agcacttgga agaagctata aatatcattc agggaattgt
1261 ggcagaaatc aacaccaaga ctggtgaatc tgaatgccgc tattataaag agcggcttct
1321 ttacttggaa gaaggccaga aagactccct gatcgacagc tctcgagtcg tgtgttgtca
1381 tggtgaactg aagaacaatc ggggcgtgaa actgcatgtt ttcctgttcc aagaagtgct
1441 tgtgatcact cgagccgtca cccacaatga gcagctttgc taccagctgt accgtcagcc
1501 aatccccgtg aaagacctcc tgctggaaga cctccaggat ggagaagtga ggctgggtgg
1561 ctccctgcga ggggcattca gcaacaatga gagaattaaa aacttcttca gagtcagttt
1621 caaaaatgga tcccaaagtc agacccactc gctacaagcc aatgacactt tcaacaaaca
1681 gcagtggctt aactgtattc gtcaagccaa agaaacagtt ttgtgtgctg ccgggcaagc
1741 tggggtgctt gactccgagg gatcgttcct aaatcccacc accgggagca gagagctaca
1801 gggagaaaca aaacttgagc agatggacca atcggacagt gagtcagact gtagtatgga
1861 cacgagtgag gtcagcctcg actgtgagcg catggaacag acagactctt cctgtgggaa
1921 cagcaggcat ggtgaaagta acgtctgaca gaagcatgtg cacttcggga agcaggcctg
1981 catcttacct gtacagtatt tgcattccac agatggaacg gtttggagaa gcactttttc
2041 atacttttgt gaaagtatac atgttggccc agtctctcgt atctgtacct ttgtccctag
2101 tactgtaact gccaatctgt ctgtgtaagc tggaatctgt ggcaactatt accctgtgtt
2161 gtatttccca agtgtctgga tggatggaga ggtactc
//