LOCUS AK293620 2523 bp mRNA linear HUM 31-JUL-2008
DEFINITION Homo sapiens cDNA FLJ53464 complete cds, highly similar to
Threonyl-tRNA synthetase, cytoplasmic (EC 6.1.1.3).
ACCESSION AK293620
VERSION AK293620.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2523)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..2523
/clone="BRACE2028375"
/clone_lib="BRACE2"
/db_xref="H-InvDB:HIT000488244"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/organism="Homo sapiens"
/tissue_type="cerebellum"
CDS 123..2393
/codon_start=1
/note="highly similar to Threonyl-tRNA synthetase,
cytoplasmic (EC 6.1.1.3)"
/protein_id="BAG57079.1"
/transl_table=1
/translation="MFEEKASSPSGKMGGEEKPIGAGEEKQKEGGKKKNKEGSGDGGR
AELNPWPEYIYTRLEMYNILKAEHDSILAEKAEKDSKPIKVTLPDGKQVDAESWKTTP
YQIACGISHTASCKNLSSLASLLASVAIPSSGMPWPPLFFLQGLADNTVIAKVNNVVW
DLDRPLEEDCTLELLKFEDEEAQAVYWHSSAHIMGEAMERVYGGCLCYGPPIENGFYY
DMYLEEGGVSSNDFSSLEALCKKIIKEKQAFERLEVKKETLLAMFKYNKFKCRILNEK
VNTPTTTVYRCGPLIDLCRGPHVRHTGKIKALKIHKNSSTYWEGKADMETLQRIYGIS
FPDPKMLKEWEKFQEEAKNRDHRKIGRDQELYFFHELSPGSCFFLPKGAYIYNALIEF
IRSEYRKRGFQEVVTPNIFNSRLWMTSGHWQHYSENMFSFEVEKELFALKPMNCPGHC
LMFDHRPRSWRELPLRLADFGVLHRNELSGALTGLTRVRRFQQDDAHIFCAMEQIEDE
IKGCLDFLRTVYSVFGFSFKLNLSTRPEKFLGDIEVWDQAEKQLENSLNEFGEKWELN
SGDGAFYGPKIDIQIKDAIGRYHQCATIQLDFQLPIRFNLTYVSHDGDDKKRPVIVHR
AILGSVERMIAILTENYGGKWPFWLSPRQVMVVPVGPTCDEYAQKVRQQFHDAKFMAD
IDLDPGCTLNKKIRNAQLAQYNFILVVGEKEKISGTVNIRTRDNKVHGERTISETIER
LQQLKEFRSKQAEEEF"
BASE COUNT 763 a 517 c 606 g 637 t
ORIGIN
1 gattgcatca gctggtccag ccgaggccaa gtcccgggcg ctagcccacc tccgacccgc
61 ctcttggctc ctctcctcta ggccgtcgct ttcgggttct ctcatcgctt cgtcgttcgc
121 caatgtttga ggagaaggcc agcagtcctt cagggaagat gggaggcgag gagaagccga
181 ttggtgctgg tgaagagaag caaaaggaag gaggcaaaaa gaagaacaaa gaaggatctg
241 gagatggagg tcgagctgag ttgaatcctt ggcctgaata tatttacaca cgtcttgaga
301 tgtataatat actaaaagca gaacatgatt ccattctggc agaaaaggca gaaaaagata
361 gcaagccaat taaagtcact ttgcctgatg gtaaacaggt tgatgcggaa tcttggaaaa
421 ctacaccata tcaaattgcc tgtggaatta gtcacacagc ttcctgcaaa aacctctctt
481 cacttgcatc tctgcttgca tctgtggcca ttccctcatc tggaatgcca tggcctcctc
541 ttttcttcct tcaaggcctg gccgacaaca ccgttattgc taaagtaaat aatgttgtgt
601 gggacctgga ccgccctctg gaagaagatt gtaccttgga gcttctcaag tttgaggatg
661 aggaagctca ggcagtgtat tggcactcta gtgctcacat aatgggtgaa gccatggaaa
721 gagtctatgg tggatgttta tgctacggtc cgccaataga aaatggattc tattatgaca
781 tgtacctcga agaagggggt gtgtctagca atgatttctc ttctctggag gctttgtgta
841 agaaaatcat taaagaaaaa caagcttttg aaagactgga agttaagaaa gaaactttac
901 tggcaatgtt taagtataac aagttcaaat gccggatatt gaatgaaaag gtgaatactc
961 caactaccac agtctataga tgtggccctt tgatagatct ctgccggggt cctcatgtta
1021 gacacacggg caaaattaag gctttaaaaa tacacaaaaa ttcctccacg tactgggaag
1081 gcaaagcaga tatggagact ctccagagaa tttatggcat ttcattccca gatcctaaaa
1141 tgttgaaaga gtgggagaag ttccaagagg aagctaaaaa ccgagatcat aggaaaattg
1201 gcagggacca agaactatat ttctttcatg aactcagccc tggaagttgc ttttttctgc
1261 caaaaggagc ctacatttat aatgcactta ttgaattcat taggagcgaa tataggaaaa
1321 gaggattcca ggaggtagtc accccaaaca tcttcaacag ccgactctgg atgacctcgg
1381 gccactggca gcactacagc gagaacatgt tctcctttga ggtggagaag gagctgtttg
1441 ccctgaaacc catgaactgc ccaggacact gccttatgtt tgatcatcgg ccaaggtcct
1501 ggcgagaact gcctctgcgg ctagctgatt ttggggtact tcataggaac gagctgtctg
1561 gagcactcac aggactcacc cgggtacgaa gattccaaca ggatgatgct cacatattct
1621 gtgccatgga gcagattgaa gatgaaataa aaggttgttt ggattttcta cgtacggtat
1681 atagcgtatt tggattttct tttaaactaa acctttctac tcgcccggaa aaattccttg
1741 gagatatcga agtatgggat caagctgaga aacaacttga aaacagtctg aatgaatttg
1801 gtgaaaagtg ggagttaaac tctggagatg gagctttcta tggcccaaag attgacatac
1861 agattaaaga tgcgattggg cggtaccacc agtgtgcaac catccagctg gatttccagt
1921 tgcccatcag atttaatctt acttatgtaa gccatgatgg tgatgataag aaaaggccag
1981 tgattgttca tcgagccatc ttgggatcag tggaaagaat gattgctatc ctcacagaaa
2041 actatggggg caaatggccc ttttggctgt cccctcgcca ggtaatggta gttccagtgg
2101 gaccaacctg tgatgaatat gcccaaaagg tacgacaaca attccacgat gccaaattca
2161 tggcagacat tgatctggat ccaggctgta cattgaataa aaagattcga aatgcacagt
2221 tagcacagta taacttcatt ttagttgttg gtgaaaaaga gaaaatcagt ggcactgtta
2281 atatccgcac aagagacaat aaggtccacg gggaacgcac catttctgaa actatcgagc
2341 ggctacagca gctcaaagag ttccgcagca aacaggcaga agaagaattt taatgaaaaa
2401 attacccaga ttggctccat ggaaaaggag gaacagcgtt tccgtaaaat tgactttgta
2461 ctctgaaaac gtcaatttat attgaacttg gaggagtttg gcaaagtctg aataggtcaa
2521 cct
//