LOCUS AK293577 2178 bp mRNA linear HUM 24-JUL-2008
DEFINITION Homo sapiens cDNA FLJ56545 complete cds, highly similar to
ATP-dependent RNA helicase DDX50 (EC 3.6.1.-).
ACCESSION AK293577
VERSION AK293577.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2178)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..2178
/clone="BRACE2021607"
/clone_lib="BRACE2"
/db_xref="H-InvDB:HIT000488201"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/organism="Homo sapiens"
/tissue_type="cerebellum"
CDS 67..1998
/codon_start=1
/note="highly similar to ATP-dependent RNA helicase DDX50
(EC 3.6.1.-)"
/protein_id="BAG57047.1"
/transl_table=1
/translation="MPGKLLWGDIMELEAPLEESESQKKERQKSDRRKSRHHYDSDEK
SETRENGVTDDLDAPKAKKSKMKEKLNGDTEEGFNRLSDEFSKSHKSRRKDLPNGDID
EYEKKSKRVSSLDTSTHKSSDNKLEETLTREQKEGAFSNFPISEETIKLLKGRGVTYL
FPIQVKTFGPVYEGKDLIAQARTGTGKTFSFAIPLIERLQRNQETIKKSRSPKVLVLA
PTRELANQVAKDFKDITRKLSVACFYGGTSYQSQINHIRNGIDILVGTPGRIKDHLQS
GRLDLSKLRHVVLDEVDQMLDLGFAEQVEDIIHESYKTDSEDNPQTLLFSATCPQWVY
KVAKKYMKSRYEQVDLVGKMTQKAATTVEHLAIQCHWSQRPAVIGDVLQVYSGSEGRA
IIFCETKKNVTEMAMNPHIKQNAQCLHGDIAQSQREITLKGFREGSFKVLVATNVAAR
GLDIPEVDLVIQSSPPQDVESYIHRSGRTGRAGRTGICICFYQPRERGQLRYVEQKAG
ITFKRVGVPSTMDLVKSKSMDAIRSLASVSYAAVDFFRPSAQRLIEEKGAVDALAAAL
AHISGASSFEPRSLITSDKGFVTMTLESLEEIQDVSCAWKELNRKLSSNAVSQITRMC
LLKGNMGVCFDVPTTESER"
BASE COUNT 706 a 400 c 516 g 556 t
ORIGIN
1 tcacgctgtc gctgcccgta ggtggttgtg gccactgtgc ccggagggag gcggcggtgg
61 ccagtaatgc ctgggaaact cctctggggg gacattatgg agctggaagc acccttggag
121 gagtccgaga gccagaagaa ggagaggcaa aagagtgaca gaaggaagtc aaggcaccat
181 tatgactcgg atgagaaatc agaaacaaga gaaaatggtg ttacagatga cctggatgct
241 cccaaggcca aaaaatctaa aatgaaagag aagctaaatg gagacactga agaaggattt
301 aatagacttt cagatgaatt ctccaaatct cataagtcaa gaagaaaaga tctaccaaat
361 ggagatatag atgaatatga aaaaaaatca aagcgagtat catctttaga tacttctact
421 cataaatcaa gtgataataa actagaggag accttaacac gtgaacagaa agaaggagcc
481 ttctccaatt ttcctatttc tgaagagact ataaagcttc tgaaaggtcg aggggtaaca
541 tatctctttc ctattcaagt taagaccttt ggtcctgtat atgaaggaaa agatttaata
601 gctcaagcac ggacaggaac aggaaagaca ttctcttttg ccatcccctt aattgaaaga
661 ctccaaagaa atcaagaaac aattaaaaaa agccgctcac caaaggtact tgttttggct
721 ccaacaaggg aactggcaaa ccaagtagcc aaagacttca aagatataac taggaaactc
781 agcgtggcgt gtttttatgg tggaacatca tatcaaagcc aaattaatca tattcgaaat
841 ggtattgaca tcttggttgg aacacctggt cgtatcaaag accatctgca gagtggccga
901 ttggatcttt ctaaactgcg acatgttgtg cttgatgaag tggatcagat gttagattta
961 ggtttcgctg aacaagttga agatattatt catgaatcct acaaaactga ttctgaagac
1021 aatcctcaga ctttactttt ttctgcaact tgcccacagt gggtatacaa agttgcaaaa
1081 aaatacatga aatccagata tgaacaggtt gaccttgttg gaaaaatgac tcaaaaggct
1141 gcaactactg tggaacattt ggccatccag tgtcattggt ctcagaggcc agcagttatt
1201 ggagatgtcc ttcaagtcta cagtgggtct gaagggaggg ctattatttt ctgtgagacc
1261 aagaagaatg taactgaaat ggccatgaat ccacacataa aacagaatgc ccagtgttta
1321 catggggaca ttgcacagtc acaaagagaa attacactaa aaggcttcag agaaggtagt
1381 tttaaagttt tggtggcaac caatgtggct gcccgtggtt tggacattcc tgaagttgac
1441 ctggtgattc aaagttctcc tcctcaggat gttgagtcct atatccatcg ctctggacgc
1501 acaggtagag ctggacggac agggatttgt atatgttttt atcaaccaag agaaagaggt
1561 caactaagat atgtggaaca aaaagcagga attactttta aacgtgtagg tgttccttct
1621 acaatggatt tagttaaatc taaaagcatg gatgccatca ggtctctggc ttccgtttct
1681 tacgctgctg ttgatttttt ccgaccatca gctcagagac tgatagaaga gaaaggtgca
1741 gtggatgcat tggctgcagc tttagcccac atttctggtg catcaagctt tgaaccacga
1801 tctttgatca cctctgataa ggggtttgtg accatgactc tggaaagcct agaggaaata
1861 caggatgtca gctgtgcttg gaaagaactt aacagaaagc tgagtagtaa tgcagtgtct
1921 cagattacca gaatgtgcct cctgaaagga aatatgggtg tttgctttga tgttcctaca
1981 actgagtcag aaaggtagac agagtcgaca aggaagtcgc tcaggaagtc gacaagatgg
2041 tagaagacga agtggggata gaaatcgatc aagaagtggg ggccacaaac ggagttttga
2101 ctgagtattt gatagttaat ctaccagtgt gagcttgcct atttctgctt aatcatgtac
2161 attatccacc aaaaatta
//