LOCUS AK293460 1548 bp mRNA linear HUM 24-JUL-2008
DEFINITION Homo sapiens cDNA FLJ52880 complete cds, highly similar to Malate
dehydrogenase, mitochondrial precursor (EC 1.1.1.37).
ACCESSION AK293460
VERSION AK293460.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1548)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..1548
/clone="BRACE2001748"
/clone_lib="BRACE2"
/db_xref="H-InvDB:HIT000488084"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/organism="Homo sapiens"
/tissue_type="cerebellum"
CDS 1..891
/codon_start=1
/note="highly similar to Malate dehydrogenase,
mitochondrial precursor (EC 1.1.1.37)"
/protein_id="BAG56955.1"
/transl_table=1
/translation="MLSALARPASAALRRSFSTSAQNNAKVAVLGASGGIGQPLSLLL
KNSPLVSRLTLYDIAHTPGVAADLSHIETKAAVKGYLGPEQLPDCLKGCDVVVIPAGV
PRKPGMTRDDLFNTNATIVATLTAACAQHCPEAMICVIANPGLDPARVNVPVIGGHAG
KTIIPLISQCTPKVDFPQDQLTALTGRIQEAGTEVVKAKAGAGSATLSMAYAGARFVF
SLVDAMNGKEGVVECSFVKSQETECTYFSTPLLLGKKGIERNLGIGKVSSFEEKMISD
AIPELKASIKKGEDFVKTLK"
BASE COUNT 330 a 437 c 399 g 382 t
ORIGIN
1 atgctctccg ccctcgcccg gcctgccagc gctgctctcc gccgcagctt cagcacctcg
61 gcccagaaca atgctaaagt agctgtgcta ggggcctctg gaggcatcgg gcagccactt
121 tcacttctcc tgaagaacag ccccttggtg agccgcctga ccctctatga tatcgcgcac
181 acacccggag tggccgcaga tctgagccac atcgagacca aagccgctgt gaaaggctac
241 ctcggacctg aacagctgcc tgactgcctg aaaggttgtg atgtggtagt tattccggct
301 ggagtcccca gaaagccagg catgacccgg gacgacctgt tcaacaccaa tgccacgatt
361 gtggccaccc tgaccgctgc ctgtgcccag cactgcccgg aagccatgat ctgcgtcatt
421 gccaatccgg gtttggatcc agctcgagtc aacgtccctg tcattggtgg ccatgctggg
481 aagaccatca tccccctgat ctctcagtgc acccccaagg tggactttcc ccaggaccag
541 ctgacagcac tcactgggcg gatccaggag gccggcacgg aggtggtcaa ggctaaagcc
601 ggagcaggct ctgccaccct ctccatggcg tatgccggcg cccgctttgt cttctccctt
661 gtggatgcaa tgaatggaaa ggaaggtgtt gtggaatgtt ccttcgttaa gtcacaggaa
721 acggaatgta cctacttctc cacaccgctg ctgcttggga aaaagggcat cgagaggaac
781 ctgggcatcg gcaaagtctc ctcttttgag gagaagatga tctcggatgc catccccgag
841 ctgaaggcct ccatcaagaa gggggaagat ttcgtgaaga ccctgaagtg agccgctgtg
901 acgggtggcc agtttcctta atttatgaag gcatcatgtc actgcaaagc cgttgcagat
961 aaactttgta ttttaatttg ctttggtgat gattactgta ttgacatcat catgccttcc
1021 aaattgtggg tggctctgtg ggcgcatcaa taaaagccgt ccttgatttt atttttcaag
1081 gtcccttctg taaatgctgt gctttcttcc ctgtgagagc caactttaga gtgtctgcta
1141 cctcttcatt accaatcaga attagatgat gtttaactgt tagactgaag cgtgacgctt
1201 tcatcagtag cttcaagaaa gtctaaattg ttaatttatg gaattggaca cagtattcag
1261 tttacccgta catgctcctc ccgcccctcc tgttggcacc cttgcatcgc ccaggcctga
1321 ttcctcctgg gggtagttca cccccacggg ttcatagttc agcggcgaat gccaggcagc
1381 tgttttctgg ctgagcaaac agcacctttc tcattgagct tcctctactg acctctgtcc
1441 cccttgggat ttcatcttct gaccgaaccc tgatgttcag tggcagagac agcccatagc
1501 cagaactgtg ggtagaccag ggttggggtg tgcggtttgg gacagccc
//