LOCUS AK293334 1035 bp mRNA linear HUM 24-JUL-2008
DEFINITION Homo sapiens cDNA FLJ58839 complete cds, highly similar to Homo
sapiens NEDD8-conjugating enzyme (NCE2), mRNA.
ACCESSION AK293334
VERSION AK293334.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1035)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..1035
/cell_line="GI1"
/cell_type="glioma separated from gliosarcoma"
/clone="BGGI11000368"
/clone_lib="BGGI11"
/db_xref="H-InvDB:HIT000487958"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/organism="Homo sapiens"
CDS 136..771
/codon_start=1
/note="highly similar to Homo sapiens NEDD8-conjugating
enzyme (NCE2), mRNA"
/protein_id="BAG56850.1"
/transl_table=1
/translation="MLTLASKLKRDDGLKGSRTAATASDSTRRVSVRDKLLVKEVAEL
EANLPCTCKVHFPDPNKLHCFQLTVTPDEGYYQGGKFQFETEVPDAYNMVPPKVKCLT
KIWHPNITETGEICLSLLREHSIDGTGWAPTRTLKDVVWGLNSLFTDLLNFDDPLNIE
AAEHHLRDKSPMLLLHRRTSGIKWMTTSNVMPDNKRGRLQAHGLCYSLSLT"
BASE COUNT 272 a 238 c 260 g 265 t
ORIGIN
1 acttccggtc ccgccgccgg gagccggtgc ggctgtgagg ggccgcgtct cgcagcagcc
61 gcccggaccg ggcatggtgt tgggcgccgg gcccgcctcg cctgtctcgg ggagctcagg
121 gtaaaggcag cagtaatgct aacgctagca agtaaactga agcgtgacga tggtctcaaa
181 gggtcccgga cggcagccac agcgtccgac tcgactcgga gggtttctgt gagagacaaa
241 ttgcttgtta aagaggttgc agaacttgaa gctaatttac cttgtacatg taaagtgcat
301 tttcctgatc caaacaagct tcattgtttt cagctaacag taaccccaga tgagggttac
361 taccagggtg gaaaatttca gtttgaaact gaagttcccg atgcgtacaa catggtgcct
421 cccaaagtga aatgcctgac caagatctgg caccccaaca tcacagagac aggggagata
481 tgtctgagtt tattgagaga acattcaatt gatggcactg gctgggctcc cacaagaaca
541 ttaaaggatg tcgtttgggg attaaactct ttgtttactg atcttttgaa ttttgatgat
601 ccactgaata ttgaagctgc agaacatcat ttgcgggaca agtccccaat gctgttactc
661 cacaggagga cttccggaat aaagtggatg actacatcaa acgttatgcc agataataaa
721 aggggacgat tgcaggccca tggactgtgt tacagtttgt ctctaacatg aaacagcaag
781 aggtagcccc ctctcccgtc ctcatgctcc ctctcagtcc cctggattgc cccagtcctg
841 tgaccatgtt gccctgaaga agaccatctt catgactgct cattgtagat ggagaattca
901 acataaatac agcaagaaaa tgtgtttggg cttctgaaga gttgtctgct taccttaaca
961 tgtttacttt tttgaacttg tactgtatag gctgttggtg aaattcttaa gaagttgtaa
1021 tgaactcaaa attga
//