LOCUS AK292643 2176 bp mRNA linear HUM 09-JAN-2008
DEFINITION Homo sapiens cDNA FLJ76956 complete cds, highly similar to Homo
sapiens ER degradation enhancer, mannosidase alpha-like 1 (EDEM1),
mRNA.
ACCESSION AK292643
VERSION AK292643.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2176)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..2176
/clone="THYMU2034843"
/clone_lib="THYMU2"
/db_xref="H-InvDB:HIT000425440"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/organism="Homo sapiens"
/tissue_type="thymus"
CDS 1..1974
/codon_start=1
/note="highly similar to Homo sapiens ER degradation
enhancer, mannosidase alpha-like 1 (EDEM1), mRNA"
/protein_id="BAF85332.1"
/transl_table=1
/translation="MQWRALVLGLVLLRLGLHGVLWLVFGLGPSMGFYQRFPLSFGFQ
RLRSPDGPASPTSGPVGRPGGVSGPSWLQPPGTGAAQSPRKAPRRPGPGMCGPANWGY
VLGGRGRGPDEYEKRYSGAFPPQLRAQMRDLARGMFVFGYDNYMAHAFPQDELNPIHC
RGRGPDRGDPSNLNINDVLGNYSLTLVDALDTLAIMGNSSEFQKAVKLVINTVSFDKD
STVQVFEATIRVLGSLLSAHRIITDSKQPFGDMTIKDYDNELLYMAHDLAVRLLPAFE
NTKTGIPYPRVNLKTGVPPDTNNETCTAGAGSLLVEFGILSRLLGDSTFEWVARRAVK
ALWNLRSNDTGLLGNVVNIQTGHWVGKQSGLGAGLDSFYEYLLKSYILFGEKEDLEMF
NAAYQSIQNYLRRGREACNEGEGDPPLYVNVNMFSGQLMNTWIDSLQAFFPGLQVLIG
DVEDAICLHAFYYAIWKRYGALPERYNWQLQAPDVLFYPLRPELVESTYLLYQATKNP
FYLHVGMDILQSLEKYTKVKCGYATLHHVIDKSTEDRMESFFLSETCKYLYLLFDEDN
PVHKSGTRYMFTTEGHIVSVDEHLRELPWKEFFSEEGGQDQGGKSVHRPKPHELKVIN
SSSNCNRVPDERRYSLPLKSIYMRQIDQMVGLI"
BASE COUNT 518 a 575 c 587 g 496 t
ORIGIN
1 atgcaatggc gagcgctcgt cctggggctg gtgctcctcc ggcttggcct ccatggagta
61 ttgtggctcg tcttcgggct ggggcccagc atgggcttct accagcgctt tccgctcagc
121 ttcggcttcc agcgtctgag gagccccgac ggccccgcgt cgcccacctc ggggcccgtg
181 ggccggcctg ggggggtatc cgggccgtcg tggctgcagc cgccggggac cggggcagcg
241 cagagcccgc gcaaggctcc gcggcgtcct gggccgggga tgtgcggccc agccaactgg
301 ggctacgtgc tgggcggccg gggccgcggc ccggacgagt acgagaagcg ctacagcggc
361 gccttccctc cgcagctgcg tgcccagatg cgcgacctgg cacggggcat gttcgtcttt
421 ggctacgaca actacatggc tcacgccttc ccccaggacg agctcaaccc catccactgc
481 cgcggccgtg ggcccgaccg cggggaccct tcaaatctga acatcaatga tgtactaggg
541 aactactcat tgactcttgt tgatgcattg gatacacttg caataatggg aaattcatcc
601 gagttccaga aagccgtcaa gttagtgatc aacacagttt catttgacaa agattccacc
661 gtccaagtct ttgaggccac gataagggtc ctgggaagcc tcctttctgc tcacagaata
721 ataactgact ccaagcagcc ctttggtgac atgacaatta aggactatga taatgagttg
781 ttatacatgg cccatgacct ggcggtgcgg ctcctccctg cttttgaaaa caccaagaca
841 gggattccat atcctcgggt gaatctaaag acaggagttc ctcctgacac caataatgag
901 acatgcacag cgggagccgg ttccctcctg gtggaatttg ggattctgag tcgactcctg
961 ggggactcca catttgagtg ggtggccaga cgagcagtga aagccctttg gaacctccgg
1021 agcaatgata caggattact aggcaatgtc gtgaacattc agacgggcca ctgggttgga
1081 aagcagagtg gcctgggtgc cgggctggac tccttctatg aatacctctt gaaatcttac
1141 attctctttg gagaaaaaga agacctagaa atgtttaatg ctgcatatca gagtattcag
1201 aactacttaa gaagagggcg ggaagcctgc aatgaaggag aaggagaccc tccactctat
1261 gtcaacgtga acatgttcag tgggcagctg atgaacacct ggattgactc tctgcaggcc
1321 tttttccctg gactgcaggt gctgatagga gatgtggaag atgccatctg ccttcatgcc
1381 ttctactatg ccatatggaa acgatatggt gccctccctg agagatataa ctggcagctg
1441 caggcccctg acgttctctt ctacccactg agaccagagt tagtggaatc cacatatctc
1501 ctctaccagg caaccaagaa tcccttctac ctccatgtag gaatggatat tctgcagagt
1561 ctggaaaagt atacaaaagt caagtgtggg tacgccacgc tgcatcacgt cattgacaag
1621 tccacagaag accggatgga gagcttcttt ctcagtgaga cctgtaaata tttgtatctg
1681 ctgtttgatg aagacaatcc agtacacaag tctggaacca gatacatgtt cacaacagag
1741 ggacacattg tatctgtgga tgagcatctt cgggaattgc catggaagga attcttctct
1801 gaagagggag ggcaggacca agggggaaag tctgtgcaca ggccgaaacc tcatgagtta
1861 aaagtcatca actccagctc caactgcaat cgtgtacctg atgagaggag gtactccctg
1921 cccttaaaga gcatctacat gcgacagatt gaccagatgg ttggtttgat ttgatctgct
1981 ctctgtgagg cctcatcttg aaccagacct taacgaccaa acccagacca tgccaaagtc
2041 cagtctgaaa tgaaagggga cagaagtctt gctgtccatg gtggtgtagg aatttctgtg
2101 caacacctca ccacgtctgg ttaatccttg cacacttcag tgtttctctc ctgttcaata
2161 aaatgccctg ttaagg
//