LOCUS AK292582 2268 bp mRNA linear HUM 09-JAN-2008
DEFINITION Homo sapiens cDNA FLJ77623 complete cds, highly similar to Homo
sapiens glutamate decarboxylase 1 (brain, 67kDa) (GAD1),
transcript variant GAD67, mRNA.
ACCESSION AK292582
VERSION AK292582.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2268)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..2268
/clone="TESTI4045187"
/clone_lib="TESTI4"
/db_xref="H-InvDB:HIT000425379"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/organism="Homo sapiens"
/tissue_type="testis"
CDS 226..2010
/codon_start=1
/note="highly similar to Homo sapiens glutamate
decarboxylase 1 (brain, 67kDa) (GAD1), transcript variant
GAD67, mRNA"
/protein_id="BAF85271.1"
/transl_table=1
/translation="MASSTPSSSATSSNAGADPNTTNLRPTTYDTWCGVAHGCTRKLG
LKICGFLQRTNSLEEKSRLVSAFKERQSSKNLLSCENSDRDARFRRTETDFSNLFARD
LLPAKNGEEQTVQFLLEVVDILLNYVRKTFDRSTKVLDFHHPHQLLEGMEGFNLELSD
HPESLEQILVDCRDTLKYGVRTGHPRFFNQLSTGLDIIGLAGEWLTSTANTNMFTYEI
APVFVLMEQITLKKMREIVGWSSKDGDGIFSPGGAISNMYSIMAARYKYFPEVKTKGM
AAVPKLVLFTSEQSHYSIKKAGAALGFGTDNVILIKCNERGKIIPADFEAKILEAKQK
GYVPFYVNATAGTTVYGAFDPIQEIADICEKYNLWLHVDAAWGGGLLMSRKHRHKLNG
IERANSVTWNPHKMMGVLLQCSAILVKEKGILQGCNQMCAGYLFQPDKQYDVSYDTGD
KAIPCGRHVDIFKFWLMWKAKGTVGFENQINKCLELAEYLYAKIKNREEFEMVFNGEP
EHTNVCFWYIPQSLRGVPDSPQRREKLHKVAPKIKALMMESGTTMVGYQPQGDKANFF
RMVISNPAATQSDIDFLIEEIERLGQDL"
BASE COUNT 611 a 552 c 584 g 521 t
ORIGIN
1 ctcttctcct ggcgctcgcg tgcgagaggg aactagcgag aacgaggagg cagctggagg
61 tgacgccggg cagattacgc ctgtcagggc cgagccgagc ggatcgctgg gcgctgtgca
121 gaggaaaggc gggagtgccc ggctcgctgt cgcagagccg agcctgtttc tgcgccggac
181 cagtcgagga ctctggacag tagaggcccc gggacgaccg agctgatggc gtcttcgacc
241 ccatcttcgt ccgcaacctc ctcgaacgcg ggagcggacc ccaataccac taacctgcgc
301 cccacaacgt acgatacctg gtgcggcgtg gcccacggat gcaccagaaa actggggctc
361 aagatctgcg gcttcttgca aaggaccaac agcctggaag agaagagtcg ccttgtgagt
421 gccttcaagg agaggcaatc ctccaagaac ctgctttcct gtgaaaacag cgaccgggat
481 gcccgcttcc ggcgcacaga gactgacttc tctaatctgt ttgctagaga tctgcttccg
541 gctaagaacg gtgaggagca aaccgtgcaa ttcctcctgg aagtggtgga catactcctc
601 aactatgtcc gcaagacatt tgatcgctcc accaaggtgc tggactttca tcacccacac
661 cagttgctgg aaggcatgga gggcttcaac ttggagctct ctgaccaccc cgagtccctg
721 gagcagatcc tggttgactg cagagacacc ttgaagtatg gggttcgcac aggtcatcct
781 cgatttttca accagctctc cactggattg gatattattg gcctagctgg agaatggctg
841 acatcaacgg ccaataccaa catgtttaca tatgaaattg caccagtgtt tgtcctcatg
901 gaacaaataa cacttaagaa gatgagagag atagttggat ggtcaagtaa agatggtgat
961 gggatatttt ctcctggggg cgccatatcc aacatgtaca gcatcatggc tgctcgctac
1021 aagtacttcc cggaagttaa gacaaagggc atggcggctg tgcctaaact ggtcctcttc
1081 acctcagaac agagtcacta ttccataaag aaagctgggg ctgcacttgg ctttggaact
1141 gacaatgtga ttttgataaa gtgcaatgaa agggggaaaa taattccagc tgattttgag
1201 gcaaaaattc ttgaagccaa acagaaggga tatgttccct tttatgtcaa tgcaactgct
1261 ggcacgactg tttatggagc ttttgatccg atacaagaga ttgcagatat atgtgagaaa
1321 tataaccttt ggttgcatgt cgatgctgcc tggggaggtg ggctgctcat gtccaggaag
1381 caccgccata aactcaacgg catagaaagg gccaactcag tcacctggaa ccctcacaag
1441 atgatgggcg tgctgttgca gtgctctgcc attctcgtca aggaaaaggg tatactccaa
1501 ggatgcaacc agatgtgtgc aggatacctc ttccagccag acaagcagta tgatgtctcc
1561 tacgacaccg gggacaaggc aattccgtgt ggccgccacg tggatatctt caagttctgg
1621 ctgatgtgga aagcaaaggg cacagtggga tttgaaaacc agatcaacaa atgcctggaa
1681 ctggctgaat acctctatgc caagattaaa aacagagaag aatttgagat ggttttcaat
1741 ggcgagcctg agcacacaaa cgtctgtttt tggtatattc cacaaagcct caggggtgtg
1801 ccagacagcc ctcaacgacg ggaaaagcta cacaaggtgg ctccaaaaat caaagccctg
1861 atgatggagt caggtacgac catggttggc taccagcccc aaggggacaa ggccaacttc
1921 ttccggatgg tcatctccaa cccagccgct acccagtctg acattgactt cctcattgag
1981 gagatagaaa gactgggcca ggatctgtaa tcatccttcg cagaacatga gtttatggga
2041 atgccttttc cctctggcac tccagaacaa acctctatat gttgctgaaa cacacaggcc
2101 atttcattga gggaaaacat aatatcttga agaatattgt taaaacctta cttaaagctt
2161 gtttgttcta gttagcagga aatagtgttc tttttaaaaa gttgcacatt aggaacagag
2221 tatatatgta cagttataca tacctctctc tatatataca tgtatagt
//