LOCUS AK292012 3277 bp mRNA linear HUM 09-JAN-2008
DEFINITION Homo sapiens cDNA FLJ78173 complete cds, highly similar to Homo
sapiens hexokinase 1 (HK1) mRNA.
ACCESSION AK292012
VERSION AK292012.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3277)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..3277
/clone="SPLEN2004821"
/clone_lib="SPLEN2"
/db_xref="H-InvDB:HIT000424809"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/organism="Homo sapiens"
/tissue_type="spleen"
CDS 102..2855
/codon_start=1
/note="highly similar to Homo sapiens hexokinase 1 (HK1)
mRNA"
/protein_id="BAF84701.1"
/transl_table=1
/translation="MIAAQLLAYYFTELKDDQVKKIDKYLYAMRLSDETLIDIMTRFR
KEMKNGLSRDFNPTATVKMLPTFVRSIPDGSEKGDFIALDLGGSSFRILRVQVNHEKN
QNVHMESEVYDTPENIVHGSGSQLFDHVAECLGDFMEKRKIKDKKLPVGFTFSFPCQQ
SKIDEAILITWTKRFKASGVEGADVVKLLNKAIKKRGDYDANIVAVVNDTVGTMMTCG
YDDQHCEVGLIIGTGTNACYMEELRHIDLVEGDEGRMCINTEWGAFGDDGSLEDIRTE
FDREIDRGSLNPGKQLFEKMVSGMYLGELVRLILVKMAKEGLLFEGRITPELLTRGKF
NTSDVSAIEKNKEGLHNAKEILTRLGVEPSDDDCVSVQHVCTIVSFRSANLVAATLGA
ILNRLRDNKGTPRLRTTVGVDGSLYKTHPQYSRRFHKTLRRLVPDSDVRFLLSESGSG
KGAAMVTAVAYRLAEQHRQIEETLAHFHLTKDMLLEVKKRMRAEMELGLRKQTHNNAV
VKMLPSFVRRTPDGTENGDFLALDLGGTNFRVLLVKIRSGKKRTVEMHNKIYAIPIEI
MQGTGEELFDHIVSCISDFLDYMGIKGPRMPLGFTFSFPCQQTSLDAGILITWTKGFK
ATDCVGHDVVTLLRDAIKRREEFDLDVVAVVNDTVGTMMTCAYEEPTCEVGLIVGTGS
NACYMEEMKNVEMVEGDQGQMCINMEWGAFGDNGCLDDIRTHYDRLVDEYSLNAGKQG
YEKMISGMYLGEIVRNILIDFTKKGFLFRGQISETLKTRGIFETKFLSQIESDRLALL
QVRAILQQLGLNSTCDDSILVKTVCGVVSRRAAQLCGAGMAAVVDKIRENRGLDRLNV
TVGVDGTLYKLHPHFSRIMHQTVKELSPKCNVSFLLSEDGSGKGAALITAVGVRLRTE
ASS"
BASE COUNT 805 a 821 c 956 g 695 t
ORIGIN
1 gaggaggagc cgccgagcag ccgccggagg accacggctc gccagggctg cggaggaccg
61 accgtcccca cgcctgccgc cccgcgaccc cgaccgccag catgatcgcc gcgcagctcc
121 tggcctatta cttcacggag ctgaaggatg accaggtcaa aaagattgac aagtatctgt
181 atgccatgcg gctctccgat gaaactctca tagatatcat gactcgcttc aggaaggaga
241 tgaagaatgg cctctcccgg gattttaatc caacagccac agtcaagatg ttgccaacat
301 tcgtaaggtc cattcctgat ggctctgaaa agggagattt cattgccctg gatcttggtg
361 ggtcttcctt tcgaattctg cgggtgcaag tgaatcatga gaaaaaccag aatgttcaca
421 tggagtccga ggtttatgac accccagaga acatcgtgca cggcagtgga agccagcttt
481 ttgatcatgt tgctgagtgc ctgggagatt tcatggagaa aaggaagatc aaggacaaga
541 agttacctgt gggattcacg ttttcttttc cttgccaaca atccaaaata gatgaggcca
601 tcctgatcac ctggacaaag cgatttaaag cgagcggagt ggaaggagca gatgtggtca
661 aactgcttaa caaagccatc aaaaagcgag gggactatga tgccaacatc gtagctgtgg
721 tgaatgacac agtgggcacc atgatgacct gtggctatga cgaccagcac tgtgaagtcg
781 gcctgatcat cggcactggc accaatgctt gctacatgga ggaactgagg cacattgatc
841 tggtggaagg agacgagggg aggatgtgta tcaatacaga atggggagcc tttggagacg
901 atggatcatt agaagacatc cggacagagt ttgacaggga gatagaccgg ggatccctca
961 accctggaaa acagctgttt gagaagatgg tcagtggcat gtacttggga gagctggttc
1021 gactgatcct agtcaagatg gccaaggagg gcctcttatt tgaagggcgg atcaccccgg
1081 agctgctcac ccgagggaag tttaacacca gtgatgtgtc agccatcgaa aagaataagg
1141 aaggcctcca caatgccaaa gaaatcctga cccgcctggg agtggagccg tccgatgatg
1201 actgtgtctc agtccagcac gtttgcacca ttgtctcatt tcgctcagcc aacttggtgg
1261 ctgccacact gggcgccatc ttgaaccgcc tgcgtgataa caagggcaca cccaggctgc
1321 ggaccacggt tggtgtcgac ggatctcttt acaagacgca cccacagtat tcccggcgtt
1381 tccacaagac tctaaggcgc ttggtgccag actccgatgt gcgcttcctc ctctcggaga
1441 gtggcagcgg caagggggct gccatggtga cggcggtggc ctaccgcttg gccgagcagc
1501 accggcagat agaggagacc ctggctcatt tccacctcac caaagacatg ctgctggagg
1561 tgaagaagag gatgcgggcc gagatggagc tggggctgag gaagcagacg cacaacaatg
1621 ccgtggttaa gatgctgccc tccttcgtcc ggagaactcc cgacgggacc gagaatggtg
1681 acttcttggc cctggatctt ggaggaacca atttccgtgt gctgctggtg aaaatccgta
1741 gtgggaaaaa gagaacggtg gaaatgcaca acaagatcta cgccattcct attgaaatca
1801 tgcagggcac tggggaagag ctgtttgatc acattgtctc ctgcatctct gacttcttgg
1861 actacatggg gatcaaaggc cccaggatgc ctctgggctt cacgttctca tttccctgcc
1921 agcagacgag tctggacgcg ggaatcttga tcacgtggac aaagggtttt aaggcaacag
1981 actgcgtggg ccacgatgta gtcaccttac taagggatgc gataaaaagg agagaggaat
2041 ttgacctgga cgtggtggct gtggtcaacg acacagtggg caccatgatg acctgtgctt
2101 atgaggagcc cacctgtgag gttggactca ttgttgggac cggcagcaat gcctgctaca
2161 tggaggagat gaagaacgtg gagatggtgg agggggacca ggggcagatg tgcatcaaca
2221 tggagtgggg ggcctttggg gacaacgggt gtctggatga tatcaggaca cactacgaca
2281 gactggtgga cgaatattcc ctaaatgctg ggaaacaagg gtatgagaag atgatcagtg
2341 gtatgtacct gggtgaaatc gtccgcaaca tcttaatcga cttcaccaag aagggattcc
2401 tcttccgagg gcagatctct gagacgctga agacccgggg catctttgag accaagtttc
2461 tctctcagat cgagagtgac cgattagcac tgctccaggt ccgggctatc ctccagcagc
2521 taggtctgaa tagcacctgc gatgacagta tcctcgtcaa gacagtgtgc ggggtggtgt
2581 ccaggagggc cgcacagctg tgtggcgcag gcatggctgc ggttgtggat aagatccgcg
2641 agaacagagg actggaccgt ctgaatgtga ctgtgggagt ggacgggaca ctctacaagc
2701 ttcatccaca cttctccaga atcatgcacc agacggtgaa ggaactgtca ccaaaatgta
2761 acgtgtcctt cctcctgtct gaggatggca gcggcaaggg ggccgccctc atcacggccg
2821 tgggcgtgcg gttacgcaca gaggcaagca gctaagagtc cgggatcccc agcctactgc
2881 ctctccagca cttctctctt caagcggcga ccccctaccc tcccagcgag ttgcgctggg
2941 agacgctggc gccagggcct gccggcgcgg ggaggaaagc aaaatccaac taatggtata
3001 tattgtaggg tacagaatag agcgtgtgct gttgataata tctctcaccc ggatccctcc
3061 tcacttgccc tgccactttg catggtttga ttttgacctg gtcccccacg tgtgaagtgt
3121 agtggcatcc atttctaatg tatgcattca tccaacagag ttatttattg gctggagatg
3181 gaaaatcaca ccacctgaca ggccttctgg gcctccaaag cccatccttg gggttccccc
3241 tccctgtgtg aaatgtatta tcaccagcag acactgc
//