LOCUS AK291940 1745 bp mRNA linear HUM 09-JAN-2008
DEFINITION Homo sapiens cDNA FLJ77348 complete cds, highly similar to Homo
sapiens mannosidase, endo-alpha (MANEA), mRNA.
ACCESSION AK291940
VERSION AK291940.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1745)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..1745
/cell_line="SK-N-MC"
/cell_type="neuroepithelioma"
/clone="SKNMC2006013"
/clone_lib="SKNMC2"
/db_xref="H-InvDB:HIT000424737"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/organism="Homo sapiens"
CDS 112..1500
/codon_start=1
/note="highly similar to Homo sapiens mannosidase,
endo-alpha (MANEA), mRNA"
/protein_id="BAF84629.1"
/transl_table=1
/translation="MAKFRRRTCIILALFILFIFSLMMGLKMLRPNTATFGAPFGLDL
LPELHQRTIHLGKNFDFQKSDRINSETNTKNLKSVEITMKPSKASELNLDELPPLNNY
LHVFYYSWYGNPQFDGKYIHWNHPVLEHWDPRIAKNYPQGRHNPPDDIGSSFYPELGS
YSSRDPSVIETHMRQMRSASIGVLALSWYPPDVNDENGEPTDNLVPTILDKAHKYNLK
VTFHIEPYSNRDDQNMYKNVKYIIDKYGNHPAFYRYKTKTGNALPMFYVYDSYITKPE
KWANLLTTSGSRSIRNSPYDGLFIALLVEEKHKYDILQSGFDGIYTYFATNGFTYGSS
HQNWASLKLFCDKYNLIFIPSVGPGYIDTSIRPWNTQNTRNRINGKYYEIGLSAALQT
RPSLISITSFNEWHEGTQIEKAVPKRTSNTVYLDYRPHKPGLYLELTRKWSEKYSKER
ATYALDRQLPVS"
BASE COUNT 568 a 343 c 299 g 535 t
ORIGIN
1 agtcggctct atgttcgcgg tcttaacctc tcctctggcc gagtccttgc aagaagtgaa
61 ttacccgacc ctgcaaaaca cttactattt tggagtttaa agtatgtcat catggcaaag
121 tttcggagaa ggacttgcat cattttggca ctttttattc tatttatttt ctctctgatg
181 atgggtttaa aaatgctgag accaaataca gctacttttg gagctccttt tggacttgac
241 cttcttccag aacttcatca acgaactatt catttgggga aaaattttga tttccaaaag
301 agtgacagaa tcaacagtga aacaaatacc aagaatttaa aaagtgttga aatcactatg
361 aaaccttcca aagcctctga acttaacttg gatgaactac cacctctgaa caattatcta
421 catgtatttt attacagttg gtatggaaat ccacagtttg atggtaaata tatacattgg
481 aatcatccag tgttagagca ttgggaccct agaatagcca agaattatcc acaagggaga
541 cacaaccctc cagatgacat tggctccagc ttttatcctg aattgggaag ttacagttct
601 cgggatcctt ctgtcataga aactcacatg agacaaatgc gctcagcttc aattggtgta
661 ctagccctct cttggtaccc acctgatgta aatgatgaaa atggagaacc tactgataac
721 ttggtaccca ctattttgga taaagctcat aaatataacc taaaggttac ttttcacata
781 gaaccatata gcaatcgaga tgatcaaaac atgtacaaaa atgtcaagta tattatagac
841 aaatatggaa atcatccggc cttttacagg tacaagacga agactggcaa tgctcttcct
901 atgttttatg tctatgattc ctatattacc aagcctgaaa aatgggccaa tctgttaacc
961 acctcagggt ctcggagtat tcgcaattct ccttatgatg gactgtttat tgcccttctg
1021 gtagaagaaa aacataagta tgatattctt caaagtggtt ttgatggaat ttacacatat
1081 tttgccacaa atggctttac ttatggctca tcacatcaga attgggctag cctaaaatta
1141 ttttgtgata aatacaactt aatatttatc ccaagtgtgg gcccaggata catagatacc
1201 agcatccgtc catggaacac gcaaaacact cggaaccgaa tcaatgggaa gtattatgaa
1261 attggtctga gtgccgcact tcagacacgc cccagcttaa tttctatcac ctcttttaat
1321 gagtggcatg aaggaactca gattgaaaaa gctgttccca aaagaaccag taatacagtg
1381 tacctagatt accgtcctca taaaccaggt ctttacctag aactgactcg caagtggtct
1441 gaaaaataca gtaaggaaag agcaacttat gcattagatc gccagctgcc tgtttcttaa
1501 tgcattgatt aaagtttaat agttatcaaa atcacctaat ttttaaaaat agctttcgtt
1561 ttgagttctg gaaagaaaac tgtcaaaatc agtatatact attagttata tttaaaaata
1621 tttttttaaa ttctttacag ataatattat acttgttacc cttcacaata ccacatgaga
1681 aaatatctga gacaaaatgt atacaaatat attccttatg gcataattta ttgcatttct
1741 gactg
//