LOCUS AK291915 1963 bp mRNA linear HUM 09-JAN-2008
DEFINITION Homo sapiens cDNA FLJ75753 complete cds, highly similar to Homo
sapiens primase, polypeptide 2A, 58kDa (PRIM2A), mRNA.
ACCESSION AK291915
VERSION AK291915.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1963)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..1963
/cell_line="SK-N-MC"
/cell_type="neuroepithelioma"
/clone="SKNMC2002005"
/clone_lib="SKNMC2"
/db_xref="H-InvDB:HIT000424712"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/organism="Homo sapiens"
CDS 82..1611
/codon_start=1
/note="highly similar to Homo sapiens primase,
polypeptide 2A, 58kDa (PRIM2A), mRNA"
/protein_id="BAF84604.1"
/transl_table=1
/translation="MEFSGRKWRKLRLAGDQRNASYPHCLQFYLQPPSENISLIEFEN
LAIDRVKLLKSVENLGVSYVKGTEQYQSKLESELRKLKFSYRENLEDEYEPRRRDHIS
HFILRLAYCQSEELRRWFIQQEMDLLRFRFSILPKDKIQDFLKDSQLQFEAISDEEKT
LREQEIVASSPSLSGLRLGFESIYKIPFADALDLFRGRKVYLEDGFAYVPLKDIVAII
LNEFRAKLSKALALTARSLPAVQSDERLQPLLNHLSHSYTGQDYSTQGNVGKISLDQI
DLLSTKSFPPCMRQLHKALRENHHLRHGGRMQYGLFLKGIGLTLEQALQFWKQEFIKG
KMDPDKFDKGYSYNIRHSFGKEGKRTDYTPFSCLKIILSNPPSQGDYHGCPFRHSDPE
LLKQKLQSYKISPGGISQILDLVKGTHYQVACQKYFEMIHNVDDCGFSLNHPNQFFCE
SQRILNGGKDIKKEPIQPETPQPKPSVQKTKDASSALASLNSSLEMDMEGLEDYFSED
S"
BASE COUNT 549 a 407 c 437 g 570 t
ORIGIN
1 atatgaactc tcccgccacc cgggaacagt ggctgccacc gtttgtgttt tcccgagttt
61 gaattcttgc aggtgaccaa gatggagttt tctggaagaa agtggaggaa gctgaggttg
121 gcaggtgacc agaggaatgc ttcctaccct cattgccttc agttttactt gcagccacct
181 tctgaaaaca tatctttaat agaatttgaa aacttggcta ttgatagagt taaattgtta
241 aaatcagttg aaaatcttgg agtgagctat gtgaaaggaa ctgaacaata ccagagtaag
301 ttggagagtg agcttcggaa gctcaagttt tcctacagag aaaacttaga agatgaatat
361 gaaccacgaa gaagagatca tatttctcat tttattttgc ggcttgctta ttgccagtct
421 gaagaactta gacgctggtt cattcaacaa gaaatggatc tccttcgatt tagatttagt
481 attttaccca aggataaaat tcaggatttc ttaaaggata gccaattgca gtttgaggct
541 ataagtgatg aagagaagac tcttcgagaa caggagattg ttgcctcatc accaagttta
601 agtggactta ggttggggtt cgagtccatt tataagatcc cttttgctga tgctctggat
661 ttgtttcgag gaaggaaagt ctatttggaa gatggctttg cttacgtacc acttaaggac
721 attgtggcaa tcatcctgaa tgaatttaga gccaaactgt ccaaggcttt ggcattaaca
781 gccaggtcct tgcctgctgt gcagtctgat gaaagacttc agcctctgct caatcacctc
841 agtcattcct acactggcca agattacagt acccagggaa atgttgggaa gatttcttta
901 gatcagattg atttgctttc taccaaatcc ttcccacctt gcatgcgtca gttacataaa
961 gccttgcggg aaaatcacca tcttcgtcat ggaggccgaa tgcagtatgg cctatttctg
1021 aagggcattg gtttaacttt ggaacaggca ttgcagttct ggaagcaaga atttatcaaa
1081 ggaaagatgg atccagacaa gtttgataaa ggttactctt acaacatccg tcacagcttt
1141 ggaaaggaag gcaagaggac agactataca cctttcagtt gcctgaagat tattctgtcc
1201 aatccaccaa gccaagggga ttatcatggg tgcccattcc gtcacagtga tccagagctg
1261 ctgaagcaaa agttgcagtc atacaagatc tctcctggag ggataagcca gattttggat
1321 ttagtaaagg ggacacatta ccaggtagcc tgtcaaaaat actttgagat gatacacaat
1381 gtggatgatt gtggcttttc tttgaatcat cctaatcagt tcttttgtga gagccaacgt
1441 attctaaatg gtggtaaaga cataaagaag gaacctatcc aaccagaaac tcctcaaccc
1501 aaaccaagtg ttcagaaaac caaggatgca tcatctgctc tggcctcttt aaattcctct
1561 ctggaaatgg atatggaagg actagaagat tactttagtg aagattctta ggcagtttta
1621 taaccctttt tcctcaatag cctgtttcct gtttttaaga ttttgccttt gttgttgaaa
1681 aagggtttca ctctgtcacc aaggcttagt gcagtgacac aattacagct gattgcagcc
1741 ttgaccttcc cagctcaagt gatcctccta cctcagcctc ccaagtagtt aggaccacag
1801 gtgtgcacct catatccaga taattttttt caattttttt ttgtagaggt ggggggtctc
1861 cctatgttgc ccaggcagat ctcagactcc tgggctcaag cgatcctcac acctcagcgt
1921 cccagagtgc tgggattacg gttgtgagcc actgtgcctg gcc
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