LOCUS AK291838 1028 bp mRNA linear HUM 09-JAN-2008
DEFINITION Homo sapiens cDNA FLJ75736 complete cds, highly similar to Homo
sapiens endothelin 1 (EDN1), mRNA.
ACCESSION AK291838
VERSION AK291838.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1028)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..1028
/cell_type="pulmonary artery endothelial cells (HPAEC)"
/clone="PUAEN2005264"
/clone_lib="PUAEN2"
/db_xref="H-InvDB:HIT000424635"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="primary culture, pulmonary artery endothelial
cells"
/organism="Homo sapiens"
CDS 272..910
/codon_start=1
/note="highly similar to Homo sapiens endothelin 1
(EDN1), mRNA"
/protein_id="BAF84527.1"
/transl_table=1
/translation="MDYLLMIFSLLFVACQGAPETAVLGAELSAVGENGGEKPTPSPP
WRLRRSKRCSCSSLMDKECVYFCHLDIIWVNTPEHVVPYGLGSPRSKRALENLLPTKA
TDRENRCQCASQKDKKCWNFCQAGKELRAEDIMEKDWNNHKKGKDCSKLGKKCIYQQL
VRGRKIRRSSEEHLRQTRSETMRNSVKSSFHDPKLKGKPSRERYVTHNRAHW"
BASE COUNT 250 a 272 c 273 g 233 t
ORIGIN
1 aaaagacgcg cctctgcatc tgcgccaggc gaacgggtcc tgcgcctcct gcagtcccag
61 ctctccaccg ccgcgtgcgc ctgcagacgc tccgctcgct gccttctctc ctggcaggcg
121 ctgccttttc tccccgttaa agggcacttg ggctgaagga tcgctttgag atctgaggaa
181 cccgcagcgc tttgagggac ctgaagctgt ttttcttcgt tttcctttgg gttcagtttg
241 aacgggaggt ttttgatccc tttttttcag aatggattat ttgctcatga ttttctctct
301 gctgtttgtg gcttgccaag gagctccaga aacagcagtc ttaggcgctg agctcagcgc
361 ggtgggtgag aacggcgggg agaaacccac tcccagtcca ccctggcggc tccgccggtc
421 caagcgctgc tcctgctcgt ccctgatgga taaagagtgt gtctacttct gccacctgga
481 catcatttgg gtcaacactc ccgagcacgt tgttccgtat ggacttggaa gccctaggtc
541 caagagagcc ttggagaatt tacttcccac aaaggcaaca gaccgtgaga atagatgcca
601 atgtgctagc caaaaagaca agaagtgctg gaatttttgc caagcaggaa aagaactcag
661 ggctgaagac attatggaga aagactggaa taatcataag aaaggaaaag actgttccaa
721 gcttgggaaa aagtgtattt atcagcagtt agtgagagga agaaaaatca gaagaagttc
781 agaggaacac ctaagacaaa ccaggtcgga gaccatgaga aacagcgtca aatcatcttt
841 tcatgatccc aagctgaaag gcaagccctc cagagagcgt tatgtgaccc acaaccgagc
901 acattggtga cagaccttcg gggcctgtct gaagccatag cctccacgga gagccctgtg
961 gccgactctg cactctccac cctggctggg atcagagcag gagcatcctc tgctggttcc
1021 tgactggc
//