LOCUS AK291826 2294 bp mRNA linear HUM 09-JAN-2008
DEFINITION Homo sapiens cDNA FLJ77774 complete cds.
ACCESSION AK291826
VERSION AK291826.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2294)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..2294
/cell_type="pulmonary artery endothelial cells (HPAEC)"
/clone="PUAEN2000531"
/clone_lib="PUAEN2"
/db_xref="H-InvDB:HIT000424623"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="primary culture, pulmonary artery endothelial
cells"
/organism="Homo sapiens"
CDS 291..2027
/codon_start=1
/protein_id="BAF84515.1"
/transl_table=1
/translation="MNISVDLETNYAELVLDVGRVTLGENSRKKMKDCKLRKKQNESV
SRAMCALLNSGGGVIKAEIENEDYSYTKDGIGLDLENSFSNILLFVPEYLDFMQNGNY
FLIFVKSWSLNTSGLRITTLSSNLYKRDITSAKVMNATAALEFLKDMKKTRGRLYLRP
ELLAKRPCVDIQEENNMKALAGVFFDRTELDRKEKLTFTESTHVEIKNFSTEKLLQRI
KEILPQYVSAFANTDGGYLFIGLNEDKEIIGFKAEMSDLDDLEREIEKSIRKMPVHHF
CMEKKKINYSCKFLGVYDKGSLCGYVCALRVERFCCAVFAKEPDSWHVKDNRVMQLTR
KEWIQFMVEAEPKFSSSYEEVISQINTSLPAPHSWPLLEWQRQRHHCPGLSGRITYTP
ENLCRKLFLQHEGLKQLICEEMDSVRKGSLIFSRSWSVDLGLQENHKVLCDALLISQD
SPPVLYTFHMVQDEEFKGYSTQTALTLKQKLAKIGGYTKKVCVMTKIFYLSPEGMTSC
QYDLRSQVIYPESYYFTRRKYLLKALFKALKRLKSLRDQFSFAENLYQIIGIDCFQKN
DKKMFKSCRRLT"
BASE COUNT 707 a 403 c 510 g 674 t
ORIGIN
1 gtggagaatg agcccgcgga ctgtagcctg ggcctcgcca agttcctttg ttctttgttt
61 tctttaaatt accttttttc tttttgtctt agaaaataca catgggtgaa aaagaaacga
121 gagctcccta gagatcactc agagctgttt tcgtgggttt ttaacttcca gttgctcaag
181 ggtccacgct ggcatctggg gtgtgctcgc acctgcgaag aatccagctt ctagggagca
241 ccgcgagctg aaaagaattt ctgcttggac actgcatagc tgctgggaaa atgaacatca
301 gtgttgattt ggaaacgaat tatgccgagt tggttctaga tgtgggaaga gtcactcttg
361 gagagaacag taggaaaaaa atgaaggatt gtaaactgag aaaaaagcag aatgaaagtg
421 tctcacgagc tatgtgtgct ctgctcaatt ctggaggggg agtgatcaag gctgaaattg
481 agaatgaaga ctatagttat acaaaagatg gaataggact agatttggaa aattctttta
541 gtaacattct gttatttgtt cctgagtact tagacttcat gcagaatggt aactactttc
601 tgatttttgt gaagtcatgg agcttgaaca cctctggtct gcggattacc accttgagct
661 ccaatttgta caaaagagat ataacatctg caaaagtcat gaatgccact gctgcactgg
721 agttcctcaa agacatgaaa aagactagag ggagattgta tttaagacca gaattgctgg
781 caaagaggcc ctgtgttgat atacaagaag aaaataacat gaaggccttg gccggggttt
841 tttttgatag aacagaactt gatcggaaag aaaaattgac ctttactgaa tccacacatg
901 ttgaaattaa aaacttctcg acagaaaagt tgttacaacg aattaaagag attctccctc
961 aatatgtttc tgcatttgca aatactgatg gaggatattt gttcattggt ttaaatgaag
1021 ataaagaaat aattggcttt aaagcagaga tgagtgacct cgatgactta gaaagagaaa
1081 tcgaaaagtc cattaggaag atgcctgtgc atcacttctg tatggagaag aagaagataa
1141 attattcatg caaattcctt ggagtatatg ataaaggaag tctttgtgga tatgtctgtg
1201 cactcagagt ggagcgcttc tgctgtgcag tgtttgctaa agagcctgat tcctggcatg
1261 tgaaagataa ccgtgtgatg cagttgacca ggaaggaatg gatccagttc atggtggagg
1321 ctgaaccaaa attttccagt tcatatgaag aggtgatctc tcaaataaat acgtcattac
1381 ctgctcccca cagttggcct cttttggaat ggcaacggca gagacatcac tgtccagggc
1441 tatcaggaag gataacgtat actccagaaa acctttgcag aaaactgttc ttacaacatg
1501 aaggacttaa gcaattaata tgtgaagaaa tggactctgt cagaaagggc tcactgatct
1561 tctctaggag ctggtctgtg gatctgggct tgcaagagaa ccacaaagtc ctctgtgatg
1621 ctcttctgat ttcccaggac agtcctccag tcctatacac cttccacatg gtacaggatg
1681 aggagtttaa aggctattct acacaaactg ccctaacctt aaagcagaag ctggcaaaaa
1741 ttggtggtta cactaaaaaa gtgtgtgtca tgacaaagat cttctacttg agccctgaag
1801 gcatgacaag ctgccagtat gatttaaggt cgcaagtaat ttaccctgaa tcctactatt
1861 ttacaagaag gaaatacttg ctgaaagccc tttttaaagc cttaaagaga ctcaagtctc
1921 tgagagacca gttttccttt gcagaaaatc tataccagat aatcggtata gattgctttc
1981 agaagaatga taaaaagatg tttaaatctt gtcgaaggct cacctgatgg aaaatggact
2041 gggctactga gatatttttc attatatatt tgataacatt ctctaattct gtgaaaatat
2101 ttctttgaaa actttgcaag ttaagcaact taatgtgatg ttggataatt gggttttgtc
2161 tattttcact tctccctaaa taatcttcac agatattgtt tgagggatat taggaaaatt
2221 aatttgttaa ctcgtctgtg cacagtatta tttactctgt ctgtagttcc tgaataaatt
2281 ttcttccatg cttg
//