LOCUS AK291500 1905 bp mRNA linear HUM 09-JAN-2008
DEFINITION Homo sapiens cDNA FLJ76051 complete cds, highly similar to Homo
sapiens mitogen-activated protein kinase kinase 1 (MAP2K1), mRNA.
ACCESSION AK291500
VERSION AK291500.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1905)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..1905
/cell_type="peripheral blood mononuclear cells
(HPBMC5939)"
/clone="PEBLM2002264"
/clone_lib="PEBLM2"
/db_xref="H-InvDB:HIT000424297"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="primary culture, peripheral blood mononuclear
cells"
/organism="Homo sapiens"
CDS 437..1618
/codon_start=1
/note="highly similar to Homo sapiens mitogen-activated
protein kinase kinase 1 (MAP2K1), mRNA"
/protein_id="BAF84189.1"
/transl_table=1
/translation="MPKKKPTPIQLNPAPDGSAVNGTSSAETNLEALQKKLEELELDE
QQRKRLEAFLTQKQKVGELKDDDFEKISELGAGNGGVVFKVSHKPSGLVMARKLIHLE
IKPAIRNQIIRELQVLHECNSPYIVGFYGAFYSDGEISICMEHMDGGSLDQVLKKAGR
IPEQILGKVSIAVIKGLTYLREKHKIMHRDVKPSNILVNSRGEIKLCDFGVSGQLIDS
MANSFVGTRSYMSPERLQGTHYSVQSDIWSMGLSLVEMAVGRYPIPPPDAKELELMFG
CQVEGDAAETPPRPRTPGRPLSSYGMDSRPPMAIFELLDYIVNEPPPKLPSGVFSLEF
QDFVNKCLIKNPAERADLKQLMVHAFIKRSDAEEVDFAGWLCSTIGLNQPSTPTHAAG
V"
BASE COUNT 424 a 501 c 573 g 407 t
ORIGIN
1 agtccctccc agggccgctt cgcagagcgg ctaggagcac ggcggcggcg gcactttccc
61 cggcaggagc tggagctggg ctctggtgcg cgcgcggctg tgccgcccga gccggaggga
121 ctggttggtt gagagagaga gaggaaggga atcccgggct gccgaaccgc acgttcagcc
181 cgctccgctc ctgcagggca gcctttcggc tctctgcgcg cgaagccgag tcccgggcgg
241 gtggggcggg ggtccactga gaccgctacc ggcccctcgg cgctgacggg accgcgcggg
301 gcgcacccgc tgaaggcagc cccggggccc gcggcccgga cttggtcctg cccagcgggc
361 gcggggcagc gcagcgggag gaagcgagag gtgctgccct ccccccggag ttggaagcgc
421 gttacccggg tccaaaatgc ccaagaagaa gccgacgccc atccagctga acccggcccc
481 cgacggctct gcagttaacg ggaccagctc tgcggagacc aacttggagg ccttgcagaa
541 gaagctggag gagctagagc ttgatgagca gcagcgaaag cgccttgagg cctttcttac
601 ccagaagcag aaggtgggag aactgaagga tgacgacttt gagaagatca gtgagctggg
661 ggctggcaat ggcggtgtgg tgttcaaggt ctcccacaag ccttctggcc tggtcatggc
721 cagaaagcta attcatctgg agatcaaacc cgcaatccgg aaccagatca taagggagct
781 gcaggttctg catgagtgca actctccgta catcgtgggc ttctatggtg cgttctacag
841 cgatggcgag atcagtatct gcatggagca catggatgga ggttctctgg atcaagtcct
901 gaagaaagct ggaagaattc ctgaacaaat tttaggaaaa gttagcattg ctgtaataaa
961 aggcctgaca tatctgaggg agaagcacaa gatcatgcac agagatgtca agccctccaa
1021 catcctagtc aactcccgtg gggagatcaa gctctgtgac tttggggtca gcgggcagct
1081 catcgactcc atggccaact ccttcgtggg cacaaggtcc tacatgtcgc cagaaagact
1141 ccaggggact cattactctg tgcagtcaga catctggagc atgggactgt ctctggtaga
1201 gatggcggtt gggaggtatc ccatccctcc tccagatgcc aaggagctgg agctgatgtt
1261 tgggtgccag gtggaaggag atgcggctga gaccccaccc aggccaagga cccccgggag
1321 gccccttagc tcatacggaa tggacagccg acctcccatg gcaatttttg agttgttgga
1381 ttacatagtc aacgagcctc ctccaaaact gcccagtgga gtgttcagtc tggaatttca
1441 agattttgtg aataaatgct taataaaaaa ccccgcagag agagcagatt tgaagcaact
1501 catggttcat gcttttatca agagatctga tgctgaggaa gtggattttg caggttggct
1561 ctgctccacc atcggcctta accagcccag cacaccaacc catgctgctg gcgtctaagt
1621 gtttgggaag caacaaagag cgagtcccct gcccggtggt ttgccatgtc gcttttgggc
1681 ctccttccca tgcctgtctc tgttcagatg tgcatttcac ctgtgacaaa ggatgaagaa
1741 cacagcatgt gccaagattc tactcttgtc atttttaata ttactgtctt tattcttatt
1801 actattattg ttcccctaag tggattggct ttgtgcttgg ggctatttgt gtgtatgctg
1861 atgatcaaaa cctgtgccag gctgaattac agtgaaattt tggtg
//