LOCUS AK291476 1870 bp mRNA linear HUM 09-JAN-2008
DEFINITION Homo sapiens cDNA FLJ77308 complete cds, highly similar to Homo
sapiens ceramide kinase (CERK), transcript variant 1, mRNA.
ACCESSION AK291476
VERSION AK291476.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1870)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..1870
/clone="OCBBF3025707"
/clone_lib="OCBBF3"
/db_xref="H-InvDB:HIT000424273"
/db_xref="taxon:9606"
/dev_stage="fetal"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/organism="Homo sapiens"
/tissue_type="brain"
CDS 64..1677
/codon_start=1
/note="highly similar to Homo sapiens ceramide kinase
(CERK), transcript variant 1, mRNA"
/protein_id="BAF84165.1"
/transl_table=1
/translation="MGATGAAEPLQSVLWVKQQRCAVSLEPARALLRWWRSPGPGAGA
PGADACSVPVSEIIAVEETDVHGKHQGSGKWQKMEKPYAFTVHCVKRARRHRWKWAQV
TFWCPEEQLCHLWLQTLREMLEKLTSRPKHLLVFINPFGGKGQGKRIYERKVAPLFTL
ASITTDIIVTEHANQAKETLYEINIDKYDGIVCVGGDGMFSEVLHGLIGRTQRSAGVD
QNHPRAVLVPSSLRIGIIPAGSTDCVCYSTVGTSDAETSALHIVVGDSLAMDVSSVHH
NSTLLRYSVSLLGYGFYGDIIKDSEKKRWLGLARYDFSGLKTFLSHHCYEGTVSFLPA
QHTVGSPRDRKPCRAGCFVCRQSKQQLEEEQKKALYGLEAAEDVEEWQVVCGKFLAIN
ATNMSCACRRSPRGLSPAAHLGDGSSDLILIRKCSRFNFLRFLIRHTNQQDQFDFTFV
EVYRVKKFQFTSKHMEDEDSDLKEGGKKRFGHICSSHPSCCCTVSNSSWNCDGEVLHS
PAIEVRVHCQLVRLFARGIEENPKPDSHS"
BASE COUNT 434 a 489 c 545 g 402 t
ORIGIN
1 aacggtccgg cgcccctcgg cgtccgcgcg cccccagcct ggcggacgag cccggcggcg
61 gagatggggg cgacgggggc ggcggagccg ctgcaatccg tgctgtgggt gaagcagcag
121 cgctgcgccg tgagcctgga gcccgcgcgg gctctgctgc gctggtggcg gagcccgggg
181 cccggagccg gcgcccccgg cgcggatgcc tgctctgtgc ctgtatctga gatcatcgcc
241 gttgaggaaa cagacgttca cgggaaacat caaggcagtg gaaaatggca gaaaatggaa
301 aagccttacg cttttacagt tcactgtgta aagagagcac gacggcaccg ctggaagtgg
361 gcgcaggtga ctttctggtg tccagaggag cagctgtgtc acttgtggct gcagaccctg
421 cgggagatgc tggagaagct gacgtccaga ccaaagcatt tactggtatt tatcaacccg
481 tttggaggaa aaggacaagg caagcggata tatgaaagaa aagtggcacc actgttcacc
541 ttagcctcca tcaccactga catcatcgtt actgaacatg ctaatcaggc caaggagact
601 ctgtatgaga ttaacataga caaatacgac ggcatcgtct gtgtcggcgg agatggtatg
661 ttcagcgagg tgctgcacgg tctgattggg aggacgcaga ggagcgccgg ggtcgaccag
721 aaccaccccc gggctgtgct ggtccccagt agcctccgga ttggaatcat tcccgcaggg
781 tcaacggact gcgtgtgtta ctccaccgtg ggcaccagcg acgcagaaac ctcggcgctg
841 catatcgttg ttggggactc gctggccatg gatgtgtcct cagtccacca caacagcaca
901 ctccttcgct actccgtgtc cctgctgggc tacggcttct acggggacat catcaaggac
961 agtgagaaga aacggtggtt gggtcttgcc agatacgact tttcaggttt aaagaccttc
1021 ctctcccacc actgctatga agggacagtg tccttcctcc ctgcacaaca cacggtggga
1081 tctccaaggg ataggaagcc ctgccgggca ggatgctttg tttgcaggca aagcaagcag
1141 cagctggagg aggagcagaa gaaagcactg tatggtttgg aagctgcgga ggacgtggag
1201 gagtggcaag tcgtctgtgg gaagtttctg gccatcaatg ccacaaacat gtcctgtgct
1261 tgtcgccgga gccccagggg cctctccccg gctgcccact tgggagacgg gtcttctgac
1321 ctcatcctca tccggaaatg ctccaggttc aattttctga gatttctcat caggcacacc
1381 aaccagcagg accagtttga cttcactttt gttgaagttt atcgcgtcaa gaaattccag
1441 tttacgtcga agcacatgga ggatgaggac agcgacctca aggagggggg gaagaagcgc
1501 tttgggcaca tttgcagcag ccacccctcc tgctgctgca ccgtctccaa cagctcctgg
1561 aactgcgacg gggaggtcct gcacagccct gccatcgagg tcagagtcca ctgccagctg
1621 gttcgactct ttgcacgagg aattgaagag aatccgaagc cagactcaca cagctgagaa
1681 gccggcgtcc tgctctcaaa ctgggaaagt gtgaaaacta tttaagataa ttattacaga
1741 ccaattatgt tgatatatac atttaaatgt agaaatttat ttttgatagt taaatcttga
1801 ttttagaaga aaaccctttt gtcaacaatt ttgtgtacat atttggcatt ttcagttctg
1861 tacgcatctg
//