LOCUS AK291281 4124 bp mRNA linear HUM 09-JAN-2008
DEFINITION Homo sapiens cDNA FLJ78646 complete cds, highly similar to Homo
sapiens polymerase (DNA directed), gamma (POLG), mRNA.
ACCESSION AK291281
VERSION AK291281.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 4124)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..4124
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RP8007747"
/clone_lib="NT2RP8"
/db_xref="H-InvDB:HIT000424078"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells after
5-weeks retinoic acid (RA) induction."
/organism="Homo sapiens"
CDS 302..4021
/codon_start=1
/note="highly similar to Homo sapiens polymerase (DNA
directed), gamma (POLG), mRNA"
/protein_id="BAF83970.1"
/transl_table=1
/translation="MSRLLWRKVAGATVGPGPVPAPGRWVSSSVPASDPSDGQRRRQQ
QQQQQQQQQQQPQQPQVLSSEGGQLRHNPLDIQMLSRGLHEQIFGQGGEMPGEAAVRR
SVEHLQKHGLWGQPAVPLPDVELRLPPLYEDNLDQHFRLLAQKQSLPYLEAANLLLQA
QLPPKPPAWAWAEGWTRYGPEGEAVPVAIPEERALVFDVEVCLAEGTCPTLAVAISPS
AWYSWCSQRLVEERYSWTSQLSPADLIPLEVPTGASSPTQRDWQEQLVVGHNVSFDRA
HIREQYLIQGSRMRFLDTMSMHMAISGLSSFQRSLWIAAKQGKHKVQPPTKQGQKSQR
KARRGPAISSWDWLDISSVNSLAEVHRLYVGGPPLEKEPRELFVKGTMKDIRENFQDL
MQYCAQDVWATHEVFQQQLPLFLERCPHPVTLAGMLEMGVSYLPVNQNWERYLAEAQG
TYEELQREMKKSLMDLANDACQLLSGERYKEDPWLWDLEWDLQEFKQKKAKKVKKEPA
TASKLPIEGAGAPGDPMDQEDLGPCSEEEEFQQDVMARACLQKLKGTTELLPKRPQHL
PGHPGWYRKLCPRLDDPAWTPGPSLLSLQMRVTPKLMALTWDGFPLHYSERHGWGYLV
PGRRDNLAKLPTGTTLESAGVVCPYRAIESLYRKHCLEQGKQQLMPQEAGLAEEFLLT
DNSAIWQTVEELDYLEVEAEAKMENLRAAVPGQPLALTARGGPKDTQPSYHHGNGPYN
DVDIPGCWFFKLPHKDGNSCNVGSPFAKDFLPKMEDGTLQAGPGGASGPRALEINKMI
SFWRNAHKRISSQMVVWLPRSALPRAVIRHPDYDEEGLYGAILPQVVTAGTITRRAVE
PTWLTASNARPDRVGSELKAMVQAPPGYTLVGADVDSQELWIAAVLGDAHFAGMHGCT
AFGWMTLQGRKSRGTDLHSKTATTVGISREHAKIFNYGRIYGAGQPFAERLLMQFNHR
LTQQEAAEKAQQMYAATKGLRWYRLSDEGEWLVRELNLPVDRTEGGWISLQDLRKVQR
ETARKSQWKKWEVVAERAWKGGTESEMFNKLESIATSDIPRTPVLGCCISRALEPSAV
QEEFMTSRVNWVVQSSAVDYLHLMLVAMKWLFEEFAIDGRFCISIHDEVRYLVREEDR
YRAALALQITNLLTRCMFAYKLGLNDLPQSVAFFSAVDIDRCLRKEVTMDCKTPSNPT
GMERRYGIPQGEALDIYQIIELTKGSLEKRSQPGP"
BASE COUNT 879 a 1195 c 1307 g 743 t
ORIGIN
1 agttgcggct gccagcgaag cggaccggcc gggtggaggc cacacgctac cccgaggctg
61 cgtaggccgc gcgaaggggg acgccgtgcc gtgggcctgg ggtcggggga gcagcagacc
121 gggaagcacc gtgaggaccg aggatttggg gtggaaggca ggcatggtca aacccatttc
181 actgacagga gagcagagac aggacgtgtc tctctccacg tcttccagcc agtaaaagaa
241 gccaagctgg agcccaaagc caggtgttct gactcccagc gtgggggtcc ctgcaccaac
301 catgagccgc ctgctctgga ggaaggtggc cggcgccacc gtcgggccag ggccggttcc
361 agctccgggg cgctgggtct ccagctccgt ccccgcgtcc gaccccagcg acgggcagcg
421 gcggcggcag cagcagcagc agcagcagca gcagcagcaa cagcagcctc agcagccgca
481 agtgctatcc tcggagggcg ggcagctgcg gcacaaccca ttggacatcc agatgctctc
541 gagagggctg cacgagcaaa tcttcgggca aggaggggag atgcctggcg aggccgcggt
601 gcgccgcagc gtcgagcacc tgcagaagca cgggctctgg gggcagccag ccgtgccctt
661 gcccgacgtg gagctgcgcc tgccgcccct ctacgaggac aacctggacc agcacttccg
721 cctcctggcc cagaagcaga gcctgcccta cctggaggcg gccaacttgc tgttgcaggc
781 ccagctgccc ccgaagcccc cggcttgggc ctgggcggag ggctggaccc ggtacggccc
841 cgagggggag gccgtacccg tggccatccc cgaggagcgg gccctggtgt tcgacgtgga
901 ggtctgcttg gcagagggaa cttgccccac attggcggtg gccatatccc cctcggcctg
961 gtattcctgg tgcagccagc ggctggtgga agagcgttac tcttggacca gccagctgtc
1021 gccggctgac ctcatccccc tggaggtccc tactggtgcc agcagcccca cccagagaga
1081 ctggcaggag cagttagtgg tggggcacaa tgtttccttt gaccgagctc atatcaggga
1141 gcagtacctg atccagggtt cccgcatgcg tttcctggac accatgagca tgcacatggc
1201 catctcaggg ctaagcagct tccagcgcag tctgtggata gcagccaagc agggcaaaca
1261 caaggtccag ccccccacaa agcaaggcca gaagtcccag aggaaagcca gaagaggccc
1321 agcgatctca tcctgggact ggctggacat cagcagtgtc aacagtctgg cagaggtgca
1381 cagactttat gtaggggggc ctcccttaga gaaggagcct cgagaactgt ttgtgaaggg
1441 caccatgaag gacattcgtg agaacttcca ggacctgatg cagtactgtg cccaggacgt
1501 gtgggccacc catgaggttt tccagcagca gctaccgctc ttcttggaga ggtgtcccca
1561 cccagtgact ctggccggca tgctggagat gggtgtctcc tacctgcctg tcaaccagaa
1621 ctgggagcgt tacctggcag aggcacaggg cacttatgag gagctccagc gggagatgaa
1681 gaagtcgttg atggatctgg ccaatgatgc ctgccagctg ctctcaggag agaggtacaa
1741 agaagacccc tggctctggg acctggagtg ggacctgcaa gaatttaagc agaagaaagc
1801 taagaaggtg aagaaggaac cagccacagc cagcaagttg cccatcgagg gggctggggc
1861 ccctggtgat cccatggatc aggaagacct cggcccctgc agtgaggagg aggagtttca
1921 acaagatgtc atggcccgcg cctgcttgca gaagctgaag gggaccacag agctcctgcc
1981 caagcggccc cagcaccttc ctggacaccc tggatggtac cggaagctct gcccccggct
2041 agacgaccct gcatggaccc cgggccccag cctcctcagc ctgcagatgc gggtcacacc
2101 taaactcatg gcacttacct gggatggctt ccctctgcac tactcagagc gtcatggctg
2161 gggctacttg gtgcctgggc ggcgggacaa cctggccaag ctgccgacag gtaccaccct
2221 ggagtcagct ggggtggtct gcccctacag agccatcgag tccctgtaca ggaagcactg
2281 tctcgaacag gggaagcagc agctgatgcc ccaggaggcc ggcctggcgg aggagttcct
2341 gctcactgac aatagtgcca tatggcaaac ggtagaagaa ctggattact tagaagtgga
2401 ggctgaggcc aagatggaga acttgcgagc tgcagtgcca ggtcaacccc tagctctgac
2461 tgcccgtggt ggccccaagg acacccagcc cagctatcac catggcaatg gaccttacaa
2521 cgacgtggac atccctggct gctggttttt caagctgcct cacaaggatg gtaatagctg
2581 taatgtggga agcccctttg ccaaggactt cctgcccaag atggaggatg gcaccctgca
2641 ggctggccca ggaggtgcca gtgggccccg tgctctggaa atcaacaaaa tgatttcttt
2701 ctggaggaac gcccataaac gtatcagctc ccagatggtg gtgtggctgc ccaggtcagc
2761 tctgccccgt gctgtgatca ggcaccccga ctatgatgag gaaggcctct atggggccat
2821 cctgccccaa gtggtgactg ccggcaccat cactcgccgg gctgtggagc ccacatggct
2881 caccgccagc aatgcccggc ctgaccgagt aggcagtgag ttgaaagcca tggtgcaggc
2941 cccacctggc tacacccttg tgggtgctga tgtggactcc caagagctgt ggattgcagc
3001 tgtgcttgga gacgcccact ttgccggcat gcatggctgc acagcctttg ggtggatgac
3061 actgcagggc aggaagagca ggggcactga tctacacagt aagacagcca ctactgtggg
3121 catcagccgt gagcatgcca aaatcttcaa ctacggccgc atctatggtg ctgggcagcc
3181 ctttgctgag cgcttactaa tgcagtttaa ccaccggctc acacagcagg aggcagctga
3241 gaaggcccag cagatgtacg ctgccaccaa gggcctccgc tggtatcggc tgtcggatga
3301 gggcgagtgg ctggtgaggg agttgaacct cccagtggac aggactgagg gtggctggat
3361 ttccctgcag gatctgcgca aggtccagag agaaactgca aggaagtcac agtggaagaa
3421 gtgggaggtg gttgctgaac gggcatggaa ggggggcaca gagtcagaaa tgttcaataa
3481 gcttgagagc attgctacgt ctgacatacc acgtaccccg gtgctgggct gctgcatcag
3541 ccgagccctg gagccctcgg ctgtccagga agagtttatg accagccgtg tgaattgggt
3601 ggtacagagc tctgctgttg actacttaca cctcatgctt gtggccatga agtggctgtt
3661 tgaagagttt gccatagatg ggcgcttctg catcagcatc catgacgagg ttcgctacct
3721 ggtgcgggag gaggaccgct accgcgctgc cctggccttg cagatcacca acctcttgac
3781 caggtgcatg tttgcctaca agctgggtct gaatgacttg ccccagtcag tcgccttttt
3841 cagtgcagtc gatattgacc ggtgcctcag gaaggaagtg accatggatt gtaaaacccc
3901 ttccaaccca actgggatgg aaaggagata cgggattccc cagggtgaag cgctggatat
3961 ttaccagata attgaactca ccaaaggctc cttggaaaaa cgaagccagc ctggaccata
4021 gcactgcctg gaggctctgt atttgctccc gtggagcttc atcggggtgg tgcaggctcc
4081 caaactcagg ctttcagctg tgctttttgc aaaagggctt gcct
//