LOCUS AK291266 3887 bp mRNA linear HUM 09-JAN-2008
DEFINITION Homo sapiens cDNA FLJ78645 complete cds, highly similar to Homo
sapiens DEAH (Asp-Glu-Ala-His) box polypeptide 30 (DHX30),
transcript variant 2, mRNA.
ACCESSION AK291266
VERSION AK291266.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3887)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..3887
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RP8004992"
/clone_lib="NT2RP8"
/db_xref="H-InvDB:HIT000424063"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells after
5-weeks retinoic acid (RA) induction."
/organism="Homo sapiens"
CDS 348..3815
/codon_start=1
/note="highly similar to Homo sapiens DEAH
(Asp-Glu-Ala-His) box polypeptide 30 (DHX30), transcript
variant 2, mRNA"
/protein_id="BAF83955.1"
/transl_table=1
/translation="MAASRDLLKEFPQPKNLLNSVIGRALGISHAKDKLVYVHTNGPK
KKKVTLHIKWPKSVEVEGYGSKKIDAERQAAAAACQLFKGWGLLGPRNELFDAAKYRV
LADRFGSPADSWWRPEPTMPPTSWRQLNPESIRPGGPGGLSRSLGREEEEDEEEELEE
GTIDVTDFLSMTQQDSHAPLRDSRGSSFEMTDDDSAIRALTQFPLPKNLLAKVIQIAT
SSSTAKNLMQFHTVGTKTKLSTLTLLWPCPMTFVAKGRRKAEAENKAAALACKKLKSL
GLVDRNNEPLTHAMYNLASLRELGETQRRPCTIQVPEPILRKIETFLNHYPVESSWIA
PELRLQSDDILPLGKDSGPLSDPITGKPYVPLLEAEEVRLSQSLLELWRRRGPVWQEA
PQLPVDPHRDTILNAIEQHPVVVISGDTGCGKTTRIPQLLLERYVTEGRGARCNVIIT
QPRRISAVSVAQRVSHELGPSLRRNVGFQVRLESKPPSRGGALLFCTVGILLRKLQSN
PSLEGVSHVTVDEVHERDVNTDFLLILLKGLQRLNPALRLVLMSATGDNERFSRYFGG
CPVIKVPGFMYPVKEHYLEDILAKLGKHQYLHRHRHHESEDECALDLDLVTDLVLHID
ARGEPGGILCFLPGWQEIKGVQQRLQEALGMHESKYLILPVHSNIPMMDQKAIFQQPP
VGVRKIVLATNIAETSITINDIVHVVDSGLHKEERYDLKTKVSCLETVWVSRANVIQR
RDRAGRCQSGFAYHLFPRSRLEKMVPFQVPEILRTPLENLVLQAKIHMPEKTAVEFLS
KAVDSPNIKAVDEAVILLQEIGVLDQREYLTTLGQRLAHISTDPRLAKAIVLAAIFRC
LHPLLVVVSCLTRDPFSSSLQNRAEVDKVKALLSHDSGSDHLAFVRAVAGWEEVLRWQ
DRSSRENYLEENLLYAPSLRFIHGLIKQFSENIYEAFLVGKPSDCTLASAQCNEYSEE
EELVKGVLMAGLYPNLIQVRQGKVTRQGKFKPNSVTYRTKSGNILLHKSTINREATRL
RSRWLTYFMAVKSNGSVFVRDSSQVHPLAVLLLTDGDVHIRDDGRRATISLSDSDLLR
LEGDSRTVRLLKELRRALGRMVERSLRSELAALPPSVQEEHGQLLALLAELLRGPCGS
FDVRKTADD"
BASE COUNT 855 a 1138 c 1145 g 749 t
ORIGIN
1 agttgtagtc cggccgtggt tgggggagcc gcggctcatg cgcggtgcac agaggcttgt
61 ttcacatctg taacaacagg aggaggccca gcctcgtgat gaggaatagc aaggagagaa
121 ttcagctcca gttcaaaagc ctacaaaatc tgaggctgtc attgctttta taaggattcc
181 agctttccct cctggccaga aatgttcagc ctggactcat tcagaaaaga tcgggcccag
241 cacaggcagc gtcagtgcaa acttccccca ccccgccttc cacccatgtg tgtcaaccct
301 accccaggag ggaccatctc tcgaggctga acgttaacat ttccaacatg gcagcttcta
361 gggacctatt aaaagagttc ccacagccca aaaatcttct caacagtgtg attggaagag
421 ccctcggcat ctcacatgca aaagacaaac tagtctacgt gcacacaaat ggaccgaaga
481 aaaagaaagt cacactgcac ataaaatggc ccaagagcgt ggaggtagaa ggctatggca
541 gcaagaagat cgatgctgag cggcaggctg cagctgcagc ctgccagctg ttcaagggtt
601 ggggtctgct aggtccccgg aatgagttgt ttgacgcagc caaataccga gtgctagctg
661 atcgctttgg ctcccctgcc gacagctggt ggcgtccgga acccaccatg ccccctactt
721 cctggcggca gctgaatcca gagagtattc gaccaggggg acctgggggc ctatcccgct
781 ctttaggccg ggaagaagag gaggacgagg aggaagagct agaagaaggg accatagatg
841 ttaccgactt cttgtccatg acccagcagg attcccacgc tccactcagg gactcaaggg
901 ggagttcctt tgagatgaca gatgacgaca gtgccattag ggctctgacc cagtttccac
961 ttcccaagaa ccttctggcc aaggtgattc agattgcaac gtcatcctcc acagctaaga
1021 acctcatgca gttccatact gtgggcacca agaccaagct gtctacactc accctgctct
1081 ggccctgccc catgaccttt gttgccaaag ggcgccgcaa agcagaggct gagaataagg
1141 cggcagcctt ggcctgcaag aaactgaaga gcctgggcct ggtggacagg aacaacgaac
1201 cgcttacaca cgccatgtat aacctggcct ctttgcgtga gctgggtgag acccagcgcc
1261 gaccatgcac catccaggtg cccgagccca tcctccgcaa gatagagacc ttcctgaacc
1321 attaccctgt ggagagttca tggatcgccc cagaactccg gctgcagagt gatgacatct
1381 tgcccttagg caaggactca gggcctctga gtgaccctat cacaggcaag ccctatgtgc
1441 ccctgttgga agcagaggag gtacgtctca gccagagtct gctagaactg tggcggcggc
1501 gagggccggt ctggcaggag gccccccagc tacctgtgga cccacatcgg gacaccatcc
1561 tcaacgccat tgagcagcac ccggtggtgg tcatctctgg ggacacgggc tgtgggaaga
1621 ccacgcgcat cccccagctg ttgctggagc gctatgtgac cgagggccga ggtgcccgct
1681 gcaatgttat catcacccaa cctcgccgca tctctgctgt gtctgtggca cagcgggtca
1741 gccacgaact gggcccctcc ctgcgccgga atgtgggctt ccaggtgcgg ttggaaagta
1801 agcccccatc ccgaggcggg gccctgctct tctgcactgt gggtatcctg ctgcgtaagc
1861 tgcagagcaa ccccagcctg gagggcgtga gccacgtcac cgtggatgag gtgcatgagc
1921 gggacgtgaa cacagacttt ctgctgatcc tgctcaaggg cctgcagcgg ctcaacccgg
1981 ccctgcggct ggtgctcatg agtgccacag gggacaatga gcgcttctcc cgatactttg
2041 gtggctgccc cgtcatcaag gtgcctggct tcatgtaccc agtcaaggag cactacctag
2101 aggacatcct ggccaagttg ggcaagcacc agtacctgca ccggcaccgg caccatgagt
2161 ctgaggatga atgcgcactc gatttggacc ttgtgactga tctggttctg cacatcgatg
2221 ctcgcgggga accaggtggg atcctgtgct tcctgcctgg gtggcaggag atcaaaggag
2281 tgcagcagcg cctccaggag gccctgggca tgcacgagag caagtacctc atcctgccag
2341 tgcactccaa catccccatg atggatcaga aggccatatt ccagcagcct ccagttgggg
2401 tgcgcaagat tgtcttggcc accaacattg ctgagacttc catcacaatc aatgacatcg
2461 tgcatgtggt ggacagtggg ctgcacaagg aagaacgcta tgacctgaag accaaggtgt
2521 cctgcctgga gacagtgtgg gtatcaagag ccaatgtgat ccagcgccgg gaccgggcgg
2581 gccgctgcca gtccggcttt gcctaccact tgttccctcg aagccggctg gagaaaatgg
2641 tccctttcca agtgccagag atcctgcgca cacctcttga gaacctggtg ctgcaagcga
2701 aaatccacat gcctgagaag acggcggtgg agttcctgtc caaggctgtg gacagtccaa
2761 acatcaaggc agtggacgag gctgtgatct tgctccagga gatcggggtg ctggaccagc
2821 gggagtacct gactaccctg gggcagcgcc tggctcacat ctccaccgac ccccggttgg
2881 ccaaggccat tgtgttggct gccatcttcc gttgcctgca cccactactg gtggtcgttt
2941 cctgcctcac ccgggacccc ttcagcagca gcctacagaa ccgggcagag gtggacaagg
3001 tgaaagcact gttgagccat gacagcggca gtgaccacct ggcctttgtg cgggctgtcg
3061 ccggctggga ggaggtgctg cgttggcagg accgcagctc ccgggagaat tacctggagg
3121 aaaacctgct gtacgcaccc agcctgcgct tcatccacgg actcatcaag cagttctcag
3181 agaacattta tgaggccttc ctggtgggga agccctcgga ctgcaccctg gcctccgccc
3241 agtgcaacga gtacagtgag gaggaggagc tggtgaaggg cgtgctgatg gccggcctct
3301 accccaacct catccaggtg aggcagggca aggtcacccg gcaggggaag ttcaagccca
3361 acagcgtcac atataggacc aaatcaggca acatcctgct gcacaagtcg accattaaca
3421 gggaggccac acggttacgg agccgatggc tgacgtattt catggcagtc aagtccaatg
3481 gcagcgtctt cgtccgggac tcctctcagg tgcacccgct agctgtgctg ctgctgaccg
3541 acggggacgt gcacatccgt gatgacgggc gccgggccac catctcactg agcgacagtg
3601 acctgctgcg gctggagggt gactcgcgta ccgtgcggct gctgaaggag ctgcggcggg
3661 ccctgggccg catggtggag cggagcctgc gcagcgagct ggctgcactt ccccccagcg
3721 tacaggagga gcacgggcag ctgcttgcgc tactggcaga gctgctgcga ggaccctgtg
3781 gcagctttga tgtgcgcaag acagctgacg actgagccct gcttctgctg gggctgtgta
3841 cagagtgcaa atgtttattt aaaataaagt tctatttatc ccttgtg
//