LOCUS AK291258 3477 bp mRNA linear HUM 09-JAN-2008
DEFINITION Homo sapiens cDNA FLJ75028 complete cds, highly similar to Homo
sapiens retinoblastoma 1 (including osteosarcoma) (RB1), mRNA.
ACCESSION AK291258
VERSION AK291258.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3477)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..3477
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RP8004480"
/clone_lib="NT2RP8"
/db_xref="H-InvDB:HIT000424055"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells after
5-weeks retinoic acid (RA) induction."
/organism="Homo sapiens"
CDS 188..2974
/codon_start=1
/note="highly similar to Homo sapiens retinoblastoma 1
(including osteosarcoma) (RB1), mRNA"
/protein_id="BAF83947.1"
/transl_table=1
/translation="MPPKTPRKTAATAAAAAAEPPAPPPPPPPEEDPEQDSGPEDLPL
VRLEFEETEEPDFTALCQKLKIPDHVRERAWLTWEKVSSVDGVLGGYIQKKKELWGIC
IFIAAVDLDEMSFTFTELQKNIEISVHKFFNLLKEIDTSTKVDNAMSRLLKKYDVLFA
LFSKLERTCELIYLTQPSSSISTEINSALVLKVSWITFLLAKGEVLQMEDDLVISFQL
MLCVLDYFIKLSPPMLLKEPYKTAVIPINGSPRTPRRGQNRSARIAKQLENDTRIIEV
LCKEHECNIDEVKNVYFKNFIPFMNSLGLVTSNGLPEVENLSKRYEEIYLKNKDLDAR
LFLDHDKTLQTDSIDSFETQRTPRKSNLDEEVNVIPPHTPVRTVMNTIQQLMMILNSA
SDQPSENLISYFNNCTVNPKESILKRVKDIGYIFKEKFAKAVGQGCVEIGSQRYKLGV
RLYYRVMESMLKSEEERLSIQNFSKLLNDNIFHMSLLACALEVVMATYSRSTSQNLDS
GTDLSFPWILNVLNLKAFDFYKVIESFIKAEGNLTREMIKHLERCEHRIMESLAWLSD
SPLFDLIKQSKDREGPTDHLESACPLNLPLQNNHTAADMYLSPVRSPKKKGSTTRVNS
TANAETQATSAFQTQKPLKSTSLSLFYKKVYRLAYLRLNTLCERLLSEHPELEHIIWT
LFQHTLQNEYELMRDRHLDQIMMCSMYGICKVKNIDLKFKIIVTAYKDLPHAVQETFK
RVLIKEEEYDSIIVFYNSVFMQRLKTNILQYASTRPPTLSPIPHIPRSPYKFPSSPLR
IPGGNIYISPLKSPYKISEGLPTPTKMTPRSRILVSIGESFGTSEKFQKINQMVCNSD
RVLKRSAEGSNPPKPLKKLRFDIEGSDEADGSKHLPGESKFQQKLAEMTSTRTRMQKQ
KMNDSMDTSNKEEK"
BASE COUNT 1095 a 710 c 690 g 982 t
ORIGIN
1 tttcccgcgg ttggacgcgg cgctcagttg ccgggcgggg gagggcgcgt ccggtttttc
61 tcaggggacg ttgaaattat ttttgtaacg ggagtcggga gaggacgggg cgtgccccga
121 cgtgcgcgcg cgtcgtcctc cccggcgctc ctccacagct cgctggctcc cgccgcggaa
181 aggcgtcatg ccgcccaaaa ccccccgaaa aacggccgcc accgccgccg ctgccgccgc
241 ggaacccccg gcaccgccgc cgccgccccc tcctgaggag gacccagagc aggacagcgg
301 cccggaggac ctgcctctcg tcaggcttga gtttgaagaa acagaagaac ctgattttac
361 tgcattatgt cagaaattaa agataccaga tcatgtcaga gagagagctt ggttaacttg
421 ggagaaagtt tcatctgtgg atggagtatt gggaggctat attcaaaaga aaaaggaact
481 gtggggaatc tgtatcttta ttgcagcagt tgacctagat gagatgtcgt tcacttttac
541 tgagctacag aaaaacatag aaatcagtgt ccataaattc tttaacttac taaaagaaat
601 tgataccagt accaaagttg ataatgctat gtcaagactg ttgaagaagt atgatgtatt
661 gtttgcactc ttcagcaaat tggaaaggac atgtgaactt atatatttga cacaacccag
721 cagttcgata tctactgaaa taaattctgc attggtgcta aaagtttctt ggatcacatt
781 tttattagct aaaggggaag tattacaaat ggaagatgat ctggtgattt catttcagtt
841 aatgctatgt gtccttgact attttattaa actctcacct cccatgttgc tcaaagaacc
901 atataaaaca gctgttatac ccattaatgg ttcacctcga acacccaggc gaggtcagaa
961 caggagtgca cggatagcaa aacaactaga aaatgataca agaattattg aagttctctg
1021 taaagaacat gaatgtaata tagatgaggt gaaaaatgtt tatttcaaaa attttatacc
1081 ttttatgaat tctcttggac ttgtaacatc taatggactt ccagaggttg aaaatctttc
1141 taaacgatac gaagaaattt atcttaaaaa taaagatcta gatgcaagat tatttttgga
1201 tcatgataaa actcttcaga ctgattctat agacagtttt gaaacacaga gaacaccacg
1261 aaaaagtaac cttgatgaag aggtgaatgt aattcctcca cacactccag ttaggactgt
1321 tatgaacact atccaacaat taatgatgat tttaaattca gcaagtgatc aaccttcaga
1381 aaatctgatt tcctatttta acaactgcac agtgaatcca aaagaaagta tactgaaaag
1441 agtgaaggat ataggataca tctttaaaga gaaatttgct aaagctgtgg gacagggttg
1501 tgtcgaaatt ggatcacagc gatacaaact tggagttcgc ttgtattacc gagtaatgga
1561 atccatgctt aaatcagaag aagaacgatt atccattcaa aattttagca aacttctgaa
1621 tgacaacatt tttcatatgt ctttattggc gtgcgctctt gaggttgtaa tggccacata
1681 tagcagaagt acatctcaga atcttgattc tggaacagat ttgtctttcc catggattct
1741 gaatgtgctt aatttaaaag cctttgattt ttacaaagtg atcgaaagtt ttatcaaagc
1801 agaaggcaac ttgacaagag aaatgataaa acatttagaa cgatgtgaac atcgaatcat
1861 ggaatccctt gcatggctct cagattcacc tttatttgat cttattaaac aatcaaagga
1921 ccgagaagga ccaactgatc accttgaatc tgcttgtcct cttaatcttc ctctccagaa
1981 taatcacact gcagcagata tgtatctttc tcctgtaaga tctccaaaga aaaaaggttc
2041 aactacgcgt gtaaattcta ctgcaaatgc agagacacaa gcaacctcag ccttccagac
2101 ccagaagcca ttgaaatcta cctctctttc actgttttat aaaaaagtgt atcggctagc
2161 ctatctccgg ctaaatacac tttgtgaacg ccttctgtct gagcacccag aattagaaca
2221 tatcatctgg accctcttcc agcacaccct gcagaatgag tatgaactca tgagagacag
2281 gcatttggac caaattatga tgtgttccat gtatggcata tgcaaagtga agaatataga
2341 ccttaaattc aaaatcattg taacagcata caaggatctt cctcatgctg ttcaggagac
2401 attcaaacgt gttttgatca aagaagagga gtatgattct attatagtat tctataactc
2461 ggtcttcatg cagagactga aaacaaatat tttgcagtat gcttccacca ggccccctac
2521 cttgtcacca atacctcaca ttcctcgaag cccttacaag tttcctagtt cacccttacg
2581 gattcctgga gggaacatct atatttcacc cctgaagagt ccatataaaa tttcagaagg
2641 tctgccaaca ccaacaaaaa tgactccaag atcaagaatc ttagtatcaa ttggtgaatc
2701 attcgggact tctgagaagt tccagaaaat aaatcagatg gtatgtaaca gcgaccgtgt
2761 gctcaaaaga agtgctgaag gaagcaaccc tcctaaacca ctgaaaaaac tacgctttga
2821 tattgaagga tcagatgaag cagatggaag taaacatctc ccaggagagt ccaaatttca
2881 gcagaaactg gcagaaatga cttctactcg aacacgaatg caaaagcaga aaatgaatga
2941 tagcatggat acctcaaaca aggaagagaa atgaggatct caggaccttg gtggacactg
3001 tgtacacctc tggattcatt gtctctcaca gatgtgactg tataactttc ccaggttctg
3061 tttatggcca catttaatat cttcagctct ttttgtggat ataaaatgtg cagatgcaat
3121 tgtttgggtg attcctaagc cacttgaaat gttagtcatt gttatttata caagattgaa
3181 aatcttgtgt aaatcctgcc atttaaaaag ttgtagcaga ttgtttcctc ttccaaagta
3241 aaattgctgt gctttatgga tagtaagaat ggccctagag tgggagtcct gataacccag
3301 gcctgtctga ctactttgcc ttcttttgta gcatataggt gatgtttgct cttgttttta
3361 ttaatttata tgtatatttt tttaatttaa catgaacacc cttagaaaat gtgtcctatc
3421 tatcttccaa atgcaatttg attgactgcc cattcaccaa aattatcctg aactctt
//