LOCUS AK291253 1615 bp mRNA linear HUM 09-JAN-2008
DEFINITION Homo sapiens cDNA FLJ75934 complete cds, highly similar to Homo
sapiens vacuolar protein sorting 4B (yeast) (VPS4B), mRNA.
ACCESSION AK291253
VERSION AK291253.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1615)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..1615
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RP8004227"
/clone_lib="NT2RP8"
/db_xref="H-InvDB:HIT000424050"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells after
5-weeks retinoic acid (RA) induction."
/organism="Homo sapiens"
CDS 159..1493
/codon_start=1
/note="highly similar to Homo sapiens vacuolar protein
sorting 4B (yeast) (VPS4B), mRNA"
/protein_id="BAF83942.1"
/transl_table=1
/translation="MSSTSPNLQKAIDLASKAAQEDKAGNYEEALQLYQHAVQYFLHV
VKYEAQGDKAKQSIRAKCTEYLDRAEKLKEYLKNKEKKAQKPVKEGQPSPADEKGNDS
DGEGESDGPDKKKLQNQLQGAIVIERPNVKWSDVAGLEGAKEALKEAVILPIKFPHLF
TGKRTPWRGILLFGPPGTGKSYLAKAVATEANNSTFFSISSSDLVSKWLGESEKLVKN
LFQLARENKPSIIFIDEIDSLCGSRSENESEAARRIKTEFLVQMQGVGVDNDGILVLG
ATNIPWVLDSAIRRRFEKRIYIPLPEPHARAAMFKLHLGTTQNSLTEADFRELGRETD
GYSGADISIIVRDALMQPVRKVQSATHFKKVRGPSRADPNHLVDDLLTPCSPGDPGAI
EMTWMDVPGDKLLEPVVSMSDMLRSLSNTKPTVNEHDLLKLKKFTEDFGQEG"
BASE COUNT 489 a 342 c 380 g 404 t
ORIGIN
1 gttgtctaaa acaagttccg gaagggaggc tgcccttcgc ggtccgagaa ccaccggcct
61 ccccagtttg agggctgtta ccccgtgcgc gcttcgacgt tgctgctgtt ggctctcctc
121 gcccctcgtt cccttgggaa ccgcctggga actccgccat gtcatccact tcgcccaacc
181 tccagaaagc gatagatctg gctagcaaag cagcgcaaga agacaaggct gggaactacg
241 aagaagccct tcagctctat cagcatgctg tgcagtattt tcttcatgtc gttaaatatg
301 aagcacaggg tgataaagcc aagcaaagta tcagggcaaa gtgtacagaa tatcttgata
361 gagcagaaaa actaaaggag tacctgaaaa ataaagagaa aaaagcacag aagccagtga
421 aagaaggaca gccgagtcca gcagatgaga aggggaatga cagtgatggg gaaggagaat
481 ctgatggtcc tgacaaaaag aaactacaga atcaacttca aggtgccatt gttatagaac
541 gaccaaatgt gaaatggagt gacgttgctg gacttgaagg agccaaagaa gcactgaaag
601 aggctgtgat actgcctatt aaatttcctc atctttttac aggcaagaga acaccttgga
661 ggggaatcct attatttggg ccgcctggaa caggaaagtc ctacttagcc aaagctgtag
721 caacagaagc caacaactca acattttttt caatatcttc ctctgatctt gtttctaagt
781 ggctaggtga aagtgaaaaa ctggttaaga atttattcca acttgccaga gagaacaagc
841 cctccattat cttcattgat gaaattgatt ctctctgtgg ttcaagaagt gaaaatgaaa
901 gtgaagccgc acgtagaatt aagacggagt tcctagtgca aatgcaaggg gttggtgtag
961 acaatgatgg aattttggtt ctgggagcta caaatatacc ctgggttctg gattctgcca
1021 ttaggcgaag atttgagaaa cgaatttata ttcccttgcc ggaaccccat gcccgagcag
1081 caatgtttaa actgcaccta gggaccactc agaacagtct cacggaagca gactttcggg
1141 aacttgggag ggaaacagat ggttattcag gggcagatat aagtatcatt gtacgtgatg
1201 cccttatgca gcctgttagg aaagtacagt cagctactca ttttaaaaag gttcgcggac
1261 cttcccgagc tgatcctaac catcttgtag atgatctgct aacaccttgc tctccaggtg
1321 accctggtgc cattgaaatg acatggatgg atgtccctgg agataaactt ttggagccag
1381 ttgtttccat gtcggatatg ttgcggtcac tatctaacac aaaacctaca gtcaatgaac
1441 atgacttgtt gaaattaaag aagtttacag aagattttgg tcaagaaggc taagccaaag
1501 acaaggaaga tgcttaccat atgtattctt tctttcatag atatttttgt ctatttggat
1561 cgcattaatt gtttccagta aaactctttt accacaggga aacacacatc tcact
//