LOCUS AK291234 4777 bp mRNA linear HUM 09-JAN-2008
DEFINITION Homo sapiens cDNA FLJ78421 complete cds, highly similar to Homo
sapiens RAB3 GTPase activating protein subunit 2 (non-catalytic)
(RAB3GAP2), mRNA.
ACCESSION AK291234
VERSION AK291234.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 4777)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..4777
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RP8002936"
/clone_lib="NT2RP8"
/db_xref="H-InvDB:HIT000424031"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells after
5-weeks retinoic acid (RA) induction."
/organism="Homo sapiens"
CDS 116..4297
/codon_start=1
/note="highly similar to Homo sapiens RAB3 GTPase
activating protein subunit 2 (non-catalytic) (RAB3GAP2),
mRNA"
/protein_id="BAF83923.1"
/transl_table=1
/translation="MACSIVQFCYFQDLQAARDFLFPHLREEILSGALRRDPSKSTDW
EDDGWGAWEENEPQEPEEEGNTCKTQKTSWLQDCVLSLSPTNDLMVIAREQKAVFLVP
KWKYSDKGKEEMQFAVGWSGSLNVEEGECVTSALCIPLASQKRSSTGRPDWTCIVVGF
TSGYVRFYTENGVLLLAQLLNEDPVLQLKCRTYEIPRHPGVTEQNEELSILYPAAIVT
IDGFSLFQSLRACRNQVAKAAASGNENIQPPPLAYKKWGLQDIDTIIDHASVGIMTLS
PFDQMKTASNIGGFNAAIKNSPPAMSQYITVGSNPFTGFFYALEGSTQPLLSHVALAV
ASKLTSALFNAASGWLGWKSKHEEEAVQKQKPKVEPATPLAVRFGLPDSRRHGESICL
SPCNTLAAVTDDFGRVILLDVARGIAIRMWKGYRDAQIGWIQTVEDLHERVPEKADFS
PFGNSQGPSRVAQFLVIYAPRRGILEVWSTQQGPRVGAFNVGKHCRLLYPGYKIMGLN
NVTSQSWQPQTYQICLVDPVSGSVKTVNVPFHLALSDKKSERAKDMHLVKKLAALLKT
KSPNLDLVETEIKELILDIKYPATKKQALESILASERLPFSCLRNITQTLMDTLKSQE
LESVDEGLLQFCANKLKLLQLYESVSQLNSLDFHLDTPFSDNDLALLLRLDEKELLKL
QALLEKYKQENTRTNVRFSDDKDGVLPVKTFLEYLEYEKDVLNIKKISEEEYVALGSF
FFWKCLHGESSTEDMCHTLESAGLSPQLLLSLLLSVWLSKEKDILDKPQSICCLHTML
SLLSKMKVAIDETWDSQSVSPWWQQMRTACIQSENNGAALLSAHVGHSVAAQISNNMT
EKKFSQTVLGADSEALTDSWEALSLDTEYWKLLLKQLEDCLILQTLLHSKGNTQTSKV
SSLQAEPLPRLSVKKLLEGGKGGIADSVAKWIFKQDFSPEVLKLANEERDAENPDEPK
EGVNRSFLEVSEMEMDLGAIPDLLHLAYEQFPCSLELDVLHAHCCWEYVVQWNKDPEE
ARFFVRSIEHLKQIFNAHVQNGIALMMWNTFLVKRFSAATYLMDKVGKSPKDRLCRRD
VGMSDTAMTSFLGSCLDLLQILMEADVSRDEIQVPVLDTEDAWLSVEGPISIVELALE
QKHIHYPLVEHHSILCSILYAVMRFSLKTVKPLSLFDSKGKNAFFKDLTSIQLLPSGE
MDPNFISVRQQFLLKVVSAAVQAQHSATKVKDPTEEATPTPFGKDQDWPALAVDLAHH
LQVSEDVVRRHYVGELYNYGVDHLGEEAILQVHDKEVLASQLLVLTGQRLAHALLHTQ
TKEGMELLARLPPTLCTWLKAMDPQDLQNTEVPIATTAKLVNKVIELLPEKHGQYGLA
LHLIEAVEAISLPSL"
BASE COUNT 1408 a 959 c 1078 g 1332 t
ORIGIN
1 tttctccgcc ttctgcttgg ctagagctat tggggctcgg tggcggccgc agtggggtgg
61 agggggcagg gcgtggtgag gtaaggtgag tgccgtagtg gggttccctg gagccatggc
121 ctgctccatt gtccagttct gctacttcca ggacctccag gccgcccggg acttcctctt
181 tcctcacctg cgggaggaga tcctcagcgg cgccttgcgg agggacccca gtaaatcaac
241 agactgggaa gatgatggtt ggggagcatg ggaagaaaat gaaccacaag aacctgaaga
301 agaaggaaat acttgcaaaa cacaaaaaac ttcctggctc caagattgtg ttttatcctt
361 atctccaacc aatgatctta tggtgatagc tcgagagcaa aaagctgtat ttctagtgcc
421 aaaatggaaa tatagtgata aaggaaagga agaaatgcaa tttgctgttg gctggagtgg
481 ttccttaaat gtcgaagaag gggaatgtgt aaccagtgct ctatgtatcc cactagcaag
541 ccaaaagagg agttccactg ggcgtcctga ctggacctgc attgtggtgg gttttacttc
601 aggttatgta cgcttctaca ctgagaatgg tgtgctcttg cttgcacagc ttttgaatga
661 ggacccagta cttcaactta aatgcagaac ctatgaaata ccacgacatc ccggcgtgac
721 tgagcagaat gaagagttga gtatcttata tccagctgcc attgtgacta ttgatggatt
781 tagccttttt caatctcttc gtgcttgtcg aaatcaggta gcaaaagctg cagcatcagg
841 caatgagaac atacaaccac caccattagc ttataagaaa tggggtctac aagatattga
901 cactattatt gatcatgcta gtgttggtat tatgactctg tccccctttg atcaaatgaa
961 gactgcctcc aatataggtg gatttaatgc agcaattaaa aatagtccac ctgccatgtc
1021 tcagtatatc actgtagggt ccaatccatt tactggcttc ttctatgctt tagagggaag
1081 cacacaacca ttattatccc atgttgcact agcagttgca agtaaactca cttctgcttt
1141 atttaatgct gccagtggtt ggcttggttg gaaaagtaag cacgaagaag aagctgtcca
1201 aaagcaaaag ccgaaggttg agccagctac cccattagct gtaagatttg gacttccaga
1261 ttctagacgc catggtgaaa gcatatgtct gtctccatgt aacacactgg cagcagtaac
1321 agatgatttc ggcagagtta ttttattgga tgtagctaga ggaattgcaa tacgcatgtg
1381 gaaagggtac cgcgacgcac aaattggatg gattcaaact gtagaggacc tccatgaaag
1441 agtgccagaa aaggcagatt tttccccctt tggaaattct cagggtccaa gtcgagtagc
1501 tcaattcctt gtgatctatg cgccaagaag gggaatttta gaagtgtgga gcacacagca
1561 gggacctaga gtaggagctt tcaatgtggg gaagcactgc aggctgctgt atcctggcta
1621 taaaataatg ggtttaaata atgttaccag tcagagttgg cagccacaga cttatcagat
1681 ctgtctggtt gatccagtgt ctggaagtgt gaaaacagtg aacgttccct tccatttagc
1741 actgagtgat aagaagagtg aacgagccaa ggatatgcac ctagtgaaga aactagcagc
1801 cttactgaaa acaaaatctc ccaatcttga tttggttgaa acagaaataa aggaattaat
1861 tcttgatatt aaataccctg caaccaaaaa acaagctttg gaaagcattt tggcaagtga
1921 acgtttacca ttttcttgcc ttagaaacat cactcagact ttaatggaca ctttaaaaag
1981 tcaagaactt gagtctgttg atgaaggatt gctacagttt tgtgccaata aactaaaact
2041 gctgcaactc tatgagtctg tcagtcaatt aaattccctt gattttcatt tagacacacc
2101 attctctgat aatgacttgg ctctgttact aaggcttgat gaaaaagaac tgcttaagct
2161 ccaggcatta ctagagaaat ataagcaaga gaacaccagg acaaatgttc gattttctga
2221 tgataaagat ggtgtgttgc ctgtaaaaac attcttggaa tatttagaat atgaaaagga
2281 tgtgctcaac ataaagaaaa taagtgaaga ggaatatgtg gctttaggta gtttcttttt
2341 ttggaagtgt ttgcatggag aaagctccac tgaggatatg tgtcacactt tggagtcggc
2401 tggtcttagc cctcagctgt tgttgtctct gctcctgagt gtttggcttt caaaggaaaa
2461 ggatattttg gataaaccac agtcaatctg ctgtcttcat accatgctgt ccctcctgag
2521 caagatgaaa gtggccatcg atgagacctg ggattctcag tctgtgtccc catggtggca
2581 gcagatgcgc acagcctgta ttcagtctga gaacaatgga gccgctctgt tgtctgcgca
2641 tgttgggcat tctgttgctg cacagatatc aaacaacatg acagagaaaa aattttccca
2701 aacagttttg ggtgctgatt cagaggccct cactgattcc tgggaggcac tttctcttga
2761 cactgagtac tggaaactcc ttctgaaaca gctggaggat tgtctcatac ttcagactct
2821 gcttcacagc aaagggaaca ctcagacctc caaagtgtca tcactgcagg ctgagccact
2881 tccaaggctt tctgttaaaa agttattaga aggaggaaaa ggtggcattg cagacagtgt
2941 agccaagtgg atatttaaac aggacttcag ccctgaagta ttaaaactgg ctaatgaaga
3001 aagagatgca gaaaacccag atgaacccaa agaaggtgtt aacagaagtt tccttgaggt
3061 atcagagatg gagatggact taggagccat accagactta ctgcatttag cctatgagca
3121 gtttccttgt agccttgagc tcgatgtctt gcatgcacat tgctgctggg agtacgttgt
3181 tcagtggaat aaagatccag aggaagcacg tttttttgtt aggtcaatag aacacttgaa
3241 gcaaatattt aatgcacatg ttcaaaatgg cattgcactg atgatgtgga atacgttctt
3301 agttaaaaga ttttctgctg ctacatactt aatggataag gttggaaaat caccaaaaga
3361 taggttatgc cgaagggatg tgggaatgag tgacacagca atgacatctt tcctcggctc
3421 ctgtttggat cttcttcaga tcttaatgga ggcagatgtt agcagggatg aaatacaggt
3481 gcctgtgctg gatactgagg atgcgtggct ctccgtggaa ggaccaatct ccatagtgga
3541 actggccctt gaacagaagc acatccacta cccactggtg gagcaccact ccatcctgtg
3601 ctccatcttg tatgcagtca tgaggttttc tctgaagact gtgaagccac tttcactttt
3661 tgacagtaag ggaaaaaatg catttttcaa agacctaact tcaattcagt tattacctag
3721 tggggaaatg gatccaaatt ttatttctgt acgacaacag ttcttattga aagttgtcag
3781 tgcagctgtc caggcccaac attcagccac aaaggtcaaa gatcccacag aagaggccac
3841 acccactcct tttgggaaag accaagattg gccagctcta gctgtggatt tagcccatca
3901 ccttcaagtt agtgaagatg ttgttagaag gcattatgtg ggggaactat acaactatgg
3961 agttgaccac ttaggagaag aggccattct acaggttcat gacaaagagg tccttgcctc
4021 tcagctgctg gtgctcacgg ggcaaaggct ggctcatgcg cttctccaca cccagacaaa
4081 agaaggaatg gagctgcttg ccagacttcc acccacactg tgtacttggc tgaaagcaat
4141 ggacccccag gaccttcaaa acactgaagt gccaattgca acaacagcta aactagtaaa
4201 taaagtaatt gagcttttac cagaaaaaca tgggcaatat ggtctagcct tacacctcat
4261 tgaagctgtg gaagccatat ctcttccttc tttatgacac ttacctactg gaaatagtct
4321 aaaattatga ttataacatg aagtagtgca tggttatttt acatagtaag acctggaatt
4381 ttatgggaaa agtatgtctt tttttttcct tttgtaaaat aaataaaagt atatacaact
4441 tttaaaaagt gcaaccctgg ctaaattcca ctcctttggt taatactgaa gataaaatat
4501 aaacaaatgt caactctgtt ataattaata cagatgtttt actgtttatt tctaattcaa
4561 acacttaagt agctttattg ttaaactttg ctatttaaaa ttggttataa tttagattat
4621 gttcttataa gattctatat ttcctatcta aaatgttgtt gtaattcaat ttaaatgtgt
4681 tcatgtacag tccccatcag tagcgtatag aacaccccac tctcctttag taaatgttga
4741 gaaaatgccc ttgttgtcat gctccaaatc ccacact
//