LOCUS AK291218 3262 bp mRNA linear HUM 09-JAN-2008
DEFINITION Homo sapiens cDNA FLJ78644 complete cds, highly similar to Homo
sapiens signal peptide, CUB domain, EGF-like 3 (SCUBE3), mRNA.
ACCESSION AK291218
VERSION AK291218.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3262)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..3262
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RP8001841"
/clone_lib="NT2RP8"
/db_xref="H-InvDB:HIT000424015"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells after
5-weeks retinoic acid (RA) induction."
/organism="Homo sapiens"
CDS 79..3060
/codon_start=1
/note="highly similar to Homo sapiens signal peptide, CUB
domain, EGF-like 3 (SCUBE3), mRNA"
/protein_id="BAF83907.1"
/transl_table=1
/translation="MGSGRVPGLCLLVLLVHARAAQYSKAAQDVDECVEGTDNCHIDA
ICQNTPRSYKCICKSGYTGDGKHCKDVDECEREDNAGCVHDCVNIPGNYRCTCYDGFH
LAHDGHNCLDVDECAEGNGGCQQSCVNMMGSYECHCREGFFLSDNQHTCIQRPEEGMN
CMNKNHGCAHICRETPKGGIACECRPGFELTKNQRDCKLTCNYGNGGCQHTCDDTEQG
PRCGCHIKFVLHTDGKTCIETCAVNNGGCDSKCHDAATGVHCTCPVGFMLQPDRKTCK
DIDECRLNNGGCDHICRNTVGSFECSCKKGYKLLINERNCQDIDECSFDRTCDHICVN
TPGSFQCLCHRGYLLYGITHCGDVDECSINRGGCRFGCINTPGSYQCTCPAGQGRLHW
NGKDCTEPLKCQGSPGASKAMLSCNRSGKKDTCALTCPSRARFLPESENGFTVSCGTP
SPRAAPARAGHNGNSTNSNHCHEAAVLSIKQRASFKIKDAKCRLHLRNKGKTEEAGRI
TGPGGAPCSECQVTFIHLKCDSSRKGKGRRARTPPGKEVTRLTLELEAEVRAEETTAS
CGLPCLRQRMERRLKGSLKMLRKSINQDRFLLRLAGLDYELAHKPGLVAGERAEPMES
CRPGQHRAGTKCVSCPQGTYYHGQTEQCVPCPAGTFQEREGQLSCDLCPGSDAHGPLG
ATNVTTCAGQCPPGQHSVDGFKPCQPCPRGTYQPEAGRTLCFPCGGGLTTKHEGAISF
QDCDTKVQCSPGHYYNTSIHRCIRCAMGSYQPDFRQNFCSRCPGNTSTDFDGSTSVAQ
CKNRQCGGELGEFTGYIESPNYPGNYPAGVECIWNINPPPKRKILIVVPEIFLPSEDE
CGDVLVMRKNSSPSSITTYETCQTYERPIAFTARSRKLWINFKTSEANSARGFQIPYV
TYDEDYEQLVEDIVRDGRLYASENHQEILKDKKLIKAFFEVLAHPQNYFKYTEKHKEM
LPKSFIKLLRSKVSSFLRPYK"
BASE COUNT 765 a 978 c 882 g 637 t
ORIGIN
1 gcactctccg cctcgctccc cccccctccc ctggccgcga gaccggcccc ggcggctggg
61 ccgccagtag ctccagccat gggctcgggg cgcgtacccg ggctctgcct gcttgtcctg
121 ctggtccacg cccgcgccgc ccagtacagc aaagccgcgc aagatgtgga tgagtgtgtg
181 gaggggactg acaactgcca catcgatgct atctgccaga acaccccgag gtcatacaag
241 tgcatctgca agtctggcta cacaggggac ggcaaacact gcaaagacgt ggatgagtgc
301 gagcgagagg ataatgcagg ttgtgtgcat gactgtgtca acatccctgg caattaccgg
361 tgtacctgct atgatggatt ccacctggca catgacggac acaactgtct ggatgtggac
421 gagtgtgccg agggcaacgg cggctgtcag cagagctgtg tcaacatgat gggcagctat
481 gagtgccact gccgggaagg cttcttcctc agcgacaacc agcatacctg tatccagcgg
541 ccagaagaag gaatgaattg catgaacaag aaccacggct gtgcccacat ttgccgggag
601 acacccaagg ggggtattgc ctgtgaatgc cgtcctggct ttgagcttac caagaaccaa
661 cgggactgta aattgacatg caactatggt aacggcggct gccagcacac gtgtgatgac
721 acagagcagg gtccccggtg cggctgccat atcaagttcg tgctccatac cgacgggaag
781 acatgcatcg agacctgtgc tgtcaacaac gggggctgtg acagtaagtg ccatgatgca
841 gcgactggtg tccactgcac ctgccctgtg ggcttcatgc tgcagccaga caggaagacg
901 tgcaaagata tagatgagtg ccgcttaaac aacgggggct gtgaccatat ttgccgcaac
961 acagtgggca gcttcgaatg cagttgcaag aaaggctata agcttctcat caatgagagg
1021 aactgccagg atatagacga gtgttccttt gatcgaacct gtgaccacat atgtgtcaac
1081 acaccaggaa gcttccagtg tctctgccat cgtggctacc tgttgtatgg tatcacccac
1141 tgtggggatg tggatgaatg cagcatcaac cggggaggtt gccgctttgg ctgcatcaac
1201 actcctggca gctaccagtg tacctgccca gcaggccagg gtcggctgca ctggaatggc
1261 aaagattgca cagagccact gaagtgtcag ggcagtcctg gagcctcgaa agccatgctc
1321 agctgcaacc ggtctggcaa gaaggacacc tgtgccctga cctgtccctc cagggcccga
1381 tttttgccag agtctgagaa tggcttcacg gtgagctgtg ggacccccag ccccagggct
1441 gctccagccc gagctggcca caatgggaac agcaccaact ccaaccactg ccatgaggct
1501 gcagtgctgt ccattaaaca acgggcctcc ttcaagatca aggatgccaa atgccgtttg
1561 cacctgcgaa acaaaggcaa aacagaggag gctggcagaa tcacagggcc aggtggtgcc
1621 ccctgctctg aatgccaggt caccttcatc caccttaagt gtgactcctc tcggaagggc
1681 aagggccgac gggcccggac ccctccaggc aaagaggtca caaggctcac cctggaactg
1741 gaggcagagg tcagagccga agaaaccaca gccagctgtg ggctgccctg cctccgacag
1801 cgaatggaac ggcggctgaa aggatccctg aagatgctca gaaagtccat caaccaggac
1861 cgcttcctgc tgcgcctggc aggccttgat tatgagctgg cccacaagcc gggcctggta
1921 gccggggagc gagcagagcc gatggagtcc tgtaggcccg ggcagcaccg tgctgggacc
1981 aagtgtgtca gctgcccgca gggaacgtat taccacggcc agacggagca gtgtgtgcca
2041 tgcccagcgg gcaccttcca ggagagagaa gggcagctct cctgcgacct ttgccctggg
2101 agtgatgccc acgggcctct tggagccacc aacgtcacca cgtgtgcagg tcagtgccca
2161 cctggccaac actctgtaga tgggttcaag ccctgtcagc catgcccacg tggcacctac
2221 caacctgaag caggacggac cctatgcttc ccttgtggtg ggggcctcac caccaagcat
2281 gaaggggcca tttccttcca agactgtgac accaaagtcc agtgctcccc agggcactac
2341 tacaacacca gcatccaccg ctgtattcgc tgtgccatgg gctcctatca gcccgacttc
2401 cgtcagaact tctgcagccg ctgtccagga aacacaagca cagactttga tggctctacc
2461 agtgtggccc aatgcaagaa tcgtcagtgt ggtggggagc tgggtgagtt cactggctat
2521 attgagtccc ccaactaccc gggcaactac ccagctggtg tggagtgcat ctggaacatc
2581 aaccccccac ccaagcgcaa gatccttatc gtggtaccag agatcttcct gccatctgag
2641 gatgagtgtg gggacgtcct cgtcatgaga aagaactcat ccccatcctc cattaccact
2701 tatgagacct gccagaccta cgagcgtccc attgccttca ctgcccgttc caggaagctc
2761 tggatcaact tcaagacaag cgaggccaac agcgcccgtg gcttccagat tccctatgtt
2821 acctatgatg aggactatga gcagctggta gaagacattg tgcgagatgg ccggctctat
2881 gcctctgaaa accaccagga gattttaaag gacaagaagc tcatcaaggc cttctttgag
2941 gtgctagccc acccccagaa ctacttcaag tacacagaga aacacaagga gatgctgcca
3001 aaatccttca tcaagctgct ccgctccaaa gtttccagct tcctgaggcc ctacaaatag
3061 taaccctagg ctcagagacc caatttttta agcccccaga ctccttagcc ctcagagccg
3121 gcagccccct accctcagac aaggaactct ctcctctctt tttggaggga aaaaaaaaat
3181 atcactacac aaaccaggca ctctcccttt ctgtctttct agtttccttt ccttgtctct
3241 ctctgcctgc ctctctactg tt
//