LOCUS AK291217 3598 bp mRNA linear HUM 09-JAN-2008
DEFINITION Homo sapiens cDNA FLJ76825 complete cds, highly similar to Homo
sapiens dihydropyrimidine dehydrogenase (DPYD), mRNA.
ACCESSION AK291217
VERSION AK291217.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3598)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..3598
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RP8001631"
/clone_lib="NT2RP8"
/db_xref="H-InvDB:HIT000424014"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells after
5-weeks retinoic acid (RA) induction."
/organism="Homo sapiens"
CDS 104..3181
/codon_start=1
/note="highly similar to Homo sapiens dihydropyrimidine
dehydrogenase (DPYD), mRNA"
/protein_id="BAF83906.1"
/transl_table=1
/translation="MAPVLSKDSADIESILALNPRTQTHATLRSTSAKKLDKKHWKRN
PDKNCFNCEKLENNFDDIKHTTLGERGALREAMRCLKCADAPCQKSCPTNLDIKSFIT
SIANKNYYGAAKMIFSDNPLGLTCGMVCPTSDLCVGGCNLYATEEGPINIGGLQQFAT
EVFKAMSIPQIRNPSLPPPEKMSEAYSAKIALFGAGPASISCASFLARLGYSDITIFE
KQEYVGGLSTSEIPQFRLPYDVVNFEIELMKDLGVKIICGKSLSVNEMTLSTLKEKGY
KAAFIGIGLPEPNKDAIFQGLTQDQGFYTSKDFLPLVAKGSKAGMCACHSPLPSIRGV
VIVLGAGDTAFDCATSALRCGARRVFIVFRKGFVNIRAVPEEMELAKEEKCEFLPFLS
PRKVIVKGGRIVAMQFVRTEQDETGKWNEDEDQMVHLKADVVISAFGSVLSDPKVKEA
LSPIKFNRWGLPEVDPETMQTSEAWVFAGGDVVGLANTTVESVNDGKQASWYIHKYVQ
SQYGASVSAKPELPLFYTPIDLVDISVEMAGLKFINPFGLASATPATSTSMIRRAFEA
GWGFALTKTFSLDKDIVTNVSPRIIRGTTSGPMYGPGQSSFLNIELISEKTAAYWCQS
VTELKADFPDNIVIASIMCSYNKNDWTELAKKSEDSGADALELNLSCPHGMGERGMGL
ACGQDPELVRNICRWVRQAVQIPFFAKLTPNVTDIVSIARAAKEGGANGVTATNTVSG
LMGLKSDGTPWPAVGIAKRTTYGGVSGTAIRPIALRAVTSIARALPGFPILATGGIDS
AESGLQFLHSGASVLQVCSAIQNQDFTVIEDYCTGLKALLYLKSIEGLQDWDGQSPAT
VSHQKGKPVPRIAELMDKKLPSFGPYLEQRKKIIAENKIRLKEQNVAFSPLKRNCFIP
KRPIPTIKDVIGKALQYLGTFGELSNVEQVVAMIDEEMCINCGKCYMTCNDSGYQAIQ
FDPETHLPTITDTCTGCTLCLSVCPIVDCIKMVSRTTPYEPKRGVPLSVNPVC"
BASE COUNT 1028 a 731 c 834 g 1005 t
ORIGIN
1 gttttcgact cgcgctccgg ctgctgtcac ttggctctct ggctggagct tgaggacgca
61 aggagggttt gtcactggca gactcgagac tgtaggcact gccatggccc ctgtgctcag
121 taaggactcg gcggacatcg agagtatcct ggctttaaat cctcgaacac aaactcatgc
181 aactctgcgt tccacttcgg ccaagaaatt agacaagaaa cattggaaaa gaaatcctga
241 taagaactgc tttaattgtg agaagctgga gaataatttt gatgacatca agcacacgac
301 tcttggtgag cgaggagctc tccgagaagc aatgagatgc ctgaaatgtg cagatgcccc
361 gtgtcagaag agctgtccaa ctaatcttga tattaaatca ttcatcacaa gtattgcaaa
421 caagaactat tatggagctg ctaagatgat attttctgac aacccacttg gtctgacttg
481 tggaatggta tgtccaacct ctgatctttg tgtaggtgga tgcaatttat atgccactga
541 agagggaccc attaatattg gtggattgca gcaatttgct actgaggtat tcaaagcaat
601 gagtatccca cagatcagaa atccttcgct gcctccccca gaaaaaatgt ctgaagccta
661 ttctgcaaag attgctcttt ttggtgctgg gcctgcaagt ataagttgtg cttccttttt
721 ggctcgattg gggtactctg acatcactat atttgaaaaa caagaatatg ttggtggttt
781 aagtacttct gaaattcctc agttccggct gccgtatgat gtagtgaatt ttgagattga
841 gctaatgaag gaccttggtg taaagataat ttgcggtaaa agcctttcag tgaatgaaat
901 gactcttagc actttgaaag aaaaaggcta caaagctgct ttcattggaa taggtttgcc
961 agaacccaat aaagatgcca tcttccaagg cctgacgcag gaccaggggt tttatacatc
1021 caaagacttt ttgccacttg tagccaaagg cagtaaagca ggaatgtgcg cctgtcactc
1081 tccattgcca tcgatacggg gagtcgtgat tgtacttgga gctggagaca ctgcctttga
1141 ctgtgcaaca tctgctctac gttgtggagc tcgccgtgtg ttcatcgtct tcagaaaagg
1201 ctttgttaat ataagagctg tccctgagga gatggaactt gctaaggaag aaaagtgtga
1261 atttctgcca ttcctgtccc cacggaaggt tatagtaaaa ggtgggagaa ttgttgctat
1321 gcagtttgtt cggacagagc aagatgaaac tggaaaatgg aatgaagatg aagatcagat
1381 ggtccatctg aaagccgatg tggtcatcag tgcctttggt tcagttctga gtgatcctaa
1441 agtaaaagaa gccttgagcc ctataaaatt taacagatgg ggtctcccag aagtagatcc
1501 agaaactatg caaactagtg aagcatgggt atttgcaggt ggtgatgtcg ttggtttggc
1561 taacactaca gtggaatcgg tgaatgatgg aaagcaagct tcttggtaca ttcacaaata
1621 cgtacagtca caatatggag cttccgtttc tgccaagcct gaactacccc tcttttacac
1681 tcctattgat ctggtggaca ttagtgtaga aatggccgga ttgaagttta taaatccttt
1741 tggtcttgct agcgcaactc cagccaccag cacatcaatg attcgaagag cttttgaagc
1801 tggatggggt tttgccctca ccaaaacttt ctctcttgat aaggacattg tgacaaatgt
1861 ttcccccaga atcatccggg gaaccacctc tggccccatg tatggccctg gacaaagctc
1921 ctttctgaat attgagctca tcagtgagaa aacggctgca tattggtgtc aaagtgtcac
1981 tgaactaaag gctgactttc cagacaacat tgtgattgct agcattatgt gcagttacaa
2041 taaaaatgac tggacggaac ttgccaagaa gtctgaggat tctggagcag atgccctgga
2101 gttaaattta tcatgtccac atggcatggg agaaagagga atgggcctgg cctgtgggca
2161 ggatccagag ctggtgcgga acatctgccg ctgggttagg caagctgttc agattccttt
2221 ttttgccaag ctgaccccaa atgtcactga tattgtgagc atcgcaagag ctgcaaagga
2281 aggtggtgcc aatggcgtta cagccaccaa cactgtctca ggtctgatgg gattaaaatc
2341 tgatggcaca ccttggccag cagtggggat tgcaaagcga actacatatg gaggagtgtc
2401 tgggacagca atcagaccta ttgctttgag agctgtgacc tccattgctc gtgctctgcc
2461 tggatttccc attttggcta ctggtggaat tgactctgct gaaagtggtc ttcagtttct
2521 ccatagtggt gcttccgtcc tccaggtatg cagtgccatt cagaatcagg atttcactgt
2581 gatcgaagac tactgcactg gcctcaaagc cctgctttat ctgaaaagca ttgaaggact
2641 acaagactgg gatggacaga gtccagctac tgtgagtcac cagaaaggga aaccagttcc
2701 acgtatagct gaactcatgg acaagaaact gccaagtttt ggaccttatc tggaacagcg
2761 caagaaaatc atagcagaaa acaagattag actgaaagaa caaaatgtag ctttttcacc
2821 acttaagaga aactgtttta tccccaaaag gcctattcct accatcaagg atgtaatagg
2881 aaaagcactg cagtaccttg gaacatttgg tgaattgagc aacgtagagc aagttgtggc
2941 tatgattgat gaagaaatgt gtatcaactg tggtaaatgc tacatgacct gtaatgattc
3001 tggctaccag gctatacagt ttgatccaga aacccacctg cccaccataa ccgacacttg
3061 tacaggctgt actctgtgtc tcagtgtttg ccctattgtc gactgcatca aaatggtttc
3121 caggacaaca ccttatgaac caaagagagg cgtaccctta tctgtgaatc cggtgtgtta
3181 aggtgatttg tgaaacagtt gctgtgaact ttcatgtcac ctacatatgc tgatctttta
3241 aaatcatgat ccttgtgttc agctctttcc aaattaaaac aaatatacat tttctaaata
3301 aaaatatgta atttcaaaat acatttgtaa gtgtaaaaaa tgtctcatgt caatgaccat
3361 tcaattagtg gtcataaaat agaataattc ttttctgagg atagtagtta aataactgtg
3421 tggcagttaa ttggatgttc actgccagtt gtcttatgtg aaaaattaac ttttttgtgg
3481 caattagtgt gacagtttcc aaattgccct atgctgtgct ccatatttga tttctaattg
3541 taagtgaaat taagcatttt gaaacaaagt actctttaac atacaagaaa atgtatcc
//