LOCUS AK291188 626 bp mRNA linear HUM 09-JAN-2008
DEFINITION Homo sapiens cDNA FLJ76557 complete cds, highly similar to Homo
sapiens calcitonin-related polypeptide, beta (CALCB), mRNA.
ACCESSION AK291188
VERSION AK291188.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 626)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..626
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RP7018355"
/clone_lib="NT2RP7"
/db_xref="H-InvDB:HIT000423985"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells after
5-weeks retinoic acid (RA) induction."
/organism="Homo sapiens"
CDS 77..460
/codon_start=1
/note="highly similar to Homo sapiens calcitonin-related
polypeptide, beta (CALCB), mRNA"
/protein_id="BAF83877.1"
/transl_table=1
/translation="MGFRKFSPFLALSILVLYQAGSLQAAPFRSALESSPDPATLSKE
DARLLLAALVQDYVQMKASELKQEQETQGSSSAAQKRACNTATCVTHRLAGLLSRSGG
MVKSNFVPTNVGSKAFGRRRRDLQA"
BASE COUNT 150 a 176 c 188 g 112 t
ORIGIN
1 acagcaggtg tggtgttcat cccgggtcga ccggccgctc gcgctgccct gaaactctag
61 tcgccagaga ggcggcatgg gtttccggaa gttctccccc ttcctggctc tcagtatctt
121 ggtcctgtac caggcgggca gcctccaggc ggcgccattc aggtctgccc tggagagcag
181 cccagacccg gccacactca gtaaagagga cgcgcgcctc ctgctggctg cactggtgca
241 ggactatgtg cagatgaagg ccagtgagct gaagcaggag caggagacac agggctccag
301 ctccgctgcc cagaagagag cctgcaacac tgccacctgt gtgactcatc ggctggcagg
361 cttgctgagc agatcagggg gcatggtgaa gagcaacttc gtgcccacca atgtgggttc
421 caaagccttt ggcaggcgcc gcagggacct tcaagcctga gcagatgaat gactccagga
481 agaaggtgtg tcctaaatcc aatgacatat ccttataaga gattcactca gaagacacat
541 gtggagaagg tgacatgaca gaggcaagga ggcacaagcc aaggaagtct gtgtctacca
601 gaagccagaa tcacagaaca gtctct
//