LOCUS AK291068 5650 bp mRNA linear HUM 09-JAN-2008
DEFINITION Homo sapiens cDNA FLJ78750 complete cds.
ACCESSION AK291068
VERSION AK291068.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 5650)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..5650
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RI3009595"
/clone_lib="NT2RI3"
/db_xref="H-InvDB:HIT000423865"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells treated
2-weeks mitotic inhibitor after 5-weeks retinoic acid
(RA) induction."
/note="majorly NT2 neuron"
/organism="Homo sapiens"
CDS 1039..5328
/codon_start=1
/protein_id="BAF83757.1"
/transl_table=1
/translation="MTNPWEEKVCKMAQTSLLQGKQFYCREWVFHKLQHCLQEKSNCC
DSAVNAPSLVMNSGNNASGVSGKGAAWGVLLVGGPGSGKTALCTELLWPSSPASLQRG
LHRQALAFHFCKAQDSDTLCVGGFIRGLVAQICRSGLLQGYEDKLRDPAVQSLLQPGE
CERNPAEAFKRCVLLPLLGMKPPQQSLYLLVDSVDEGCNITEGEQTSTSLSGTVAALL
AGHHEFFPPWLLLLCSARKQSKAVTKMFTGFRKISLDDLRKAYIVKDVQQYILHRLDQ
EEALRQHLTKETAEMLNQLHIKSSGCFLYLERVLDGVVENFIMLREIRDIPGTLNGLY
LWLCQRLFVRKQFAKVQPILNVILAACRPLTITELYHAVWTKNMSLTLEDFQRKLDIL
SKLLVDGLGNTKILFHYSFAEWLLDVKHCTQKYLCNAAEGHRMLAMSYTCQAKNLTPL
EAQEFALHLINSNLQLETAELALWMIWNGTPVRDSLSTLIPKEQEVLQLLVKAGAHVN
SEDDRTSCIVRQALEREDSIRTLLDNGASVNQCDSNGRTLLANAAYSGSLDVVNLLVS
RGADLEIEDAHGHTPLTLAARQGHTKVVNCLIGCGANINHTDQDGWTALRSAAWGGHT
EVVSALLYAGVKVDCADADSRTALRAAAWGGHEDIVLNLLQHGAEVNKADNEGRTALI
AAAYMGHREIVEHLLDHGAEVNHEDVDGRTALSVAALCVPASKGHASVVSLLIDRGAE
VDHCDKDGMTPLLVAAYEGHVDVVDLLLEGGADVDHTDNNGRTPLLAAASMGHASVVN
TLLFWGAAVDSIDSEGRTVLSIASAQGNVEVVRTLLDRGLDENHRDDAGWTPLHMAAF
EGHRLICEALIEQGARTNEIDNDGRIPFILASQEGHYDCVQILLENKSNIDQRGYDGR
NALRVAALEGHRDIVELLFSHGADVNCKDADGRPTLYILALENQLTMAEYFLENGANV
EASDAEGRTALHVSCWQGHMEMVQVLIAYHADVNAADNEKRSALQSAAWQGHVKVVQL
LIEHGAVVDHTCNQGATALCIAAQEGHIDVVQVLLEHGADPNHADQFGRTAMRVAAKN
GHSQIIKLLEKYGASSLNGCSPPPVHTMEQKPLQSLSSKVQSLTIKSNSSGSTGGGDM
QPSLRGLPNGPTHAFSSPSESPDSTVDRQKSSLSNNSLKSSKNSSLRTTSSTATAQTV
PIDSFHNLSFTEQIQQHSLPRSRSRQSIVSPSSTTQSLGQSHNSPSSEFEWSQVKPSL
KSTKASKGGKSENSAKSGSAGKKAKQSNSSQPKVLEYEMTQFDRRGPIAKSGTAAPPK
QMPAESQCKIMIPSAQQEIGRSQQQFLIHQQSGEQKKRNGIITNPNYHLQSNQVFLGR
VSVPRTMQDRGHQEVLEGYPSSETELSLKQALKLQIEGSDPSFNYKKETPL"
BASE COUNT 1684 a 1084 c 1343 g 1539 t
ORIGIN
1 agttactgcc ctgacggcgg cggcggcggc ggttggccgg tgggggctgg ggcgagcacg
61 accgattctg ctgggaaagc tggaactggc agccgccggg ggccaagagg aggatccgga
121 gctgggagcg tgccccgggc ccggcggagg aggcggcagc agttggagtg gagtcttggg
181 agcgctgctg gcagcgactt ctcagcgaag caagtgtctg agactgagag tccctaacag
241 cggcagcggc ggccacggcg gcggctgggg ccgagttact tgtggaaatt gttgattacc
301 cggacgtctt aaaaccaaga aacctgtgaa caggttcatt aagaatctgc tgtcttgaaa
361 aatgcatatt atgaatgctt tgaattaaga aggtgttaac agtggttgtc atttctgcca
421 cataagagac tataatctga aacagttcct ggcagttgcc cagatgcctg aaactggcac
481 gatctagtac tcttgagtga tcatccttca acgagaaata cagagctact tacatactgt
541 acctcatacc gtataaatgt aatctctctg atgccattta atgcagtgga cctcttttca
601 gaatctaatt gtcagatgca gaattaacac ttggaatctg gagaagcatt ttgtacaaat
661 tgaaagattg ggttttttct ttagttctcc acatgtattg tctcatctct gttcttgaaa
721 catccacaat cacataaact ccagttataa ccatggagtt atgtgactca ttaaaattta
781 ggaaaagtgt tgctgagctg tggaatcagg cgtttctccc tcagttcaac aactaataca
841 tagactgctg atcaagaata aggaaccaaa taaagtcatg cacgttctgt tgacagagga
901 actctgtggc tgttgaattt tactgtaact agttactctg tcttcagagt caacttaata
961 tacaagttat gtcataatgg atggaaagac ctgtgttgat gtatccagac tctagcaaat
1021 gaagataaaa aacccgttat gactaatcct tgggaagaga aagtctgcaa aatggctcaa
1081 accagtttac tgcaagggaa gcagttttac tgtagggagt gggttttcca caagcttcag
1141 cattgcctcc aggagaaaag taactgctgc gatagtgctg tcaatgcacc atcacttgta
1201 atgaattctg ggaataatgc tagtggtgtc tctggaaagg gagctgcctg gggtgtgttg
1261 ttggtaggag ggcctggcag tggcaagacg gccctatgta ctgaactctt atggccaagt
1321 tcacctgcaa gtttgcagag aggtttacat cgccaagctt tggcctttca tttctgcaaa
1381 gcccaggact ctgatacttt gtgtgttgga gggtttatta gaggtctagt agcccagatc
1441 tgccgcagtg gactactcca aggatatgag gacaagctaa gggatccagc agtccaaagc
1501 ctcttacagc ctggggagtg cgagagaaac ccagccgaag catttaaaag gtgtgttcta
1561 ctccctcttc tgggaatgaa gcctccccag caaagcctat acctgcttgt tgattctgtt
1621 gatgaagggt gtaacattac tgaaggtgaa caaacgtcta ccagcttatc tgggactgtt
1681 gcagcacttt tagctggtca ccatgagttc tttccaccat ggctattgct tctctgttct
1741 gcccgaaagc agagtaaggc tgttactaaa atgtttactg gttttcgaaa aataagttta
1801 gatgaccttc ggaaggcata tatcgtcaag gatgttcagc agtacattct tcatcgttta
1861 gatcaagaag aagctttgcg acaacacctc acaaaagaaa ctgcagagat gttaaatcaa
1921 ctgcacatta aaagcagtgg atgctttctt tacctagaac gagttttaga tggagttgta
1981 gaaaatttta ttatgttaag agaaattcgt gacatcccag gaactctaaa tggtttatat
2041 ctctggctgt gccaaagact ttttgtaaga aaacaatttg caaaggttca gcctattttg
2101 aatgtgattc ttgcagcctg ccgacctttg accataacgg aattatatca cgcagtatgg
2161 accaaaaaca tgtcgttaac tttggaagat tttcaacgca agttagatat cctctccaaa
2221 cttcttgttg atggactagg aaatacaaaa atactgtttc attatagttt tgccgagtgg
2281 cttctggatg tgaaacactg tactcagaag tatttatgta atgcagcaga aggacacaga
2341 atgttggcta tgagttatac ctgtcaagcc aagaatttaa caccattgga agcacaagaa
2401 tttgcattgc acttaattaa ctcaaactta caattagaga cagcggagtt agctctgtgg
2461 atgatatgga atggtacacc tgtcagagat tccctttcta ctttgatacc caaggaacaa
2521 gaagtgctac agctgttggt taaagctggg gctcatgtca acagtgaaga cgatcgcaca
2581 tcatgcatag ttcgacaagc cttagaaaga gaggattcca ttcggacatt attagataat
2641 ggagcttcag taaatcagtg tgattcaaat gggagaacat tattggctaa tgctgcatat
2701 agtggcagtc ttgatgtagt caatttactt gtctctaggg gagcagattt agagatagaa
2761 gatgctcatg gacatacacc actcactcta gcggctagac agggacatac caaggtggtt
2821 aattgtttga ttgggtgtgg agcaaatatt aatcatactg atcaagatgg ttggacagca
2881 ttaagatctg ctgcttgggg tggccatact gaggtagttt ctgcactact ttatgctggc
2941 gtaaaagtgg attgtgcaga tgctgatagc cgaacagctt tgagagcagc agcatgggga
3001 ggacacgagg atattgtact gaatttgcta caacatggcg ctgaagtgaa caaagctgat
3061 aatgaaggta gaactgcttt gatagcagca gcatacatgg gacatagaga gattgtggaa
3121 cacctactgg accatggagc agaagtaaat catgaggatg ttgatggcag gactgcactc
3181 tctgtagctg cactttgtgt gcctgcaagt aaagggcacg catcagttgt tagcctttta
3241 attgatcgag gtgctgaagt agatcattgt gataaagatg gcatgactcc actgctggta
3301 gctgcctatg aaggacatgt tgatgtggtt gacttgcttc tagaaggggg agcagatgta
3361 gatcacacag ataacaatgg ccgtacaccc ctcttagcag cagcgtctat gggtcatgca
3421 tcagttgtaa atacactttt gttttggggt gcagctgtgg atagtattga tagtgaaggt
3481 aggacagtcc tcagtatagc ttcagcacaa ggaaatgttg aggtggtacg tactctactg
3541 gatagagggt tagatgaaaa tcacagagat gatgctggat ggacaccttt gcacatggca
3601 gcttttgaag ggcacagatt gatatgtgaa gcacttattg aacaaggtgc tagaacaaat
3661 gagattgaca atgatggacg aatccctttc atattagctt cacaagaggg tcattatgat
3721 tgtgttcaaa tattactgga aaacaaatcc aacattgatc aaagaggtta tgatggaaga
3781 aatgcactgc gggttgctgc attagaaggg cacagggaca ttgttgaatt gctttttagc
3841 catggtgctg atgttaactg caaagatgct gatggtcggc ctacacttta tatcttggcc
3901 ttagaaaatc agcttacaat ggccgaatat tttttagaaa atggtgcaaa cgtagaagca
3961 agtgatgctg aaggaaggac agcacttcat gtgtcttgtt ggcaaggcca tatggaaatg
4021 gtgcaggtcc tgatagcata ccatgctgac gtcaatgctg cagacaatga aaagcgctct
4081 gctttgcagt ctgcagcctg gcagggccat gtaaaagtgg ttcagcttct gattgagcat
4141 ggtgctgtag ttgaccatac atgtaaccaa ggtgcaactg cactctgtat tgcagcccag
4201 gaagggcaca ttgatgttgt tcaggtctta ttagagcatg gtgctgatcc aaaccatgct
4261 gatcaatttg gacgcactgc tatgcgtgtt gcagccaaaa atggacattc tcagataatt
4321 aaattattag aaaaatatgg tgcatctagt ttgaatggct gttccccacc tcctgttcac
4381 acaatggagc aaaaacctct acagtcattg tcttcaaaag tgcagtcatt aacaattaaa
4441 tcaaatagct ctggtagtac tggtggaggg gatatgcagc cttcgttacg tggtttacct
4501 aatgggccta ctcatgcttt tagttctcct tcagaatctc cagattctac agttgaccgg
4561 cagaagtcat cactgtcaaa taattccctg aaaagctcaa aaaattcatc tttgagaact
4621 acttcatcta cagcaacggc tcaaacagtg ccaattgata gctttcataa cttgtcattt
4681 acagaacaaa ttcagcagca ttcattgcca cgcagtagaa gtcgacagtc aattgtttcc
4741 ccatcttcca caacacagtc cttaggacag agtcataatt caccaagtag tgaatttgag
4801 tggagtcaag taaagcccag tttgaagtca actaaagcaa gtaaaggggg gaaatcagaa
4861 aattctgcca agtctggatc agctgggaaa aaagcgaaac aaagtaattc ttcacagcca
4921 aaggttttag aatatgaaat gactcagttt gatagaagag gacctatagc caaatccggg
4981 actgctgcac cacctaaaca aatgccagca gaatctcaat gcaaaattat gataccttca
5041 gctcagcagg aaattggtcg atctcaacag cagtttctta ttcaccaaca aagtggggaa
5101 cagaagaaga gaaatggaat aattacaaat ccaaattatc atcttcagag caaccaggtt
5161 tttcttggta gggtttcagt cccacgaaca atgcaagata gagggcatca ggaagtgttg
5221 gagggatacc cttcctcaga gacagaatta agccttaaac aagctctgaa gcttcagatt
5281 gaaggttctg accctagctt caactataaa aaggaaacac cattataaaa gtttcctatt
5341 ctgtgaaaca gaagacattg tgatggagtg gttcttcagc tactggatgg aaacatatgc
5401 ctgttgattt gctgaaaaaa caaaaaaaat gaagaatgtg atctctgcag tacagttacc
5461 ttaattactg taatgtgcct aaatagtaag gctgccttct caatgtaacc ctctgtgctt
5521 aaaaaatttc attttgtgtg ctttgtattc actacacagg aataagcact ttttaaaaat
5581 gcagatacat actgcagttc cctgataaaa gctgaaaaga aaatttgagt attttaagtt
5641 aagatgtgat
//