LOCUS AK291060 1580 bp mRNA linear HUM 09-JAN-2008
DEFINITION Homo sapiens cDNA FLJ77114 complete cds, highly similar to Homo
sapiens v-crk sarcoma virus CT10 oncogene homolog (avian) (CRK),
transcript variant I, mRNA.
ACCESSION AK291060
VERSION AK291060.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1580)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..1580
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RI3008738"
/clone_lib="NT2RI3"
/db_xref="H-InvDB:HIT000423857"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells treated
2-weeks mitotic inhibitor after 5-weeks retinoic acid
(RA) induction."
/note="majorly NT2 neuron"
/organism="Homo sapiens"
CDS 116..730
/codon_start=1
/note="highly similar to Homo sapiens v-crk sarcoma virus
CT10 oncogene homolog (avian) (CRK), transcript variant
I, mRNA"
/protein_id="BAF83749.1"
/transl_table=1
/translation="MAGNFDSEERSSWYWGRLSRQEAVALLQGQRHGVFLVRDSSTSP
GDYVLSVSENSRVSHYIINSSGPRPPVPPSPAQPPPGVSPSRLRIGDQEFDSLPALLE
FYKIHYLDTTTLIEPVSRSRQGSGVILRQEEAEYVRALFDFNGNDEEDLPFKKGDILR
IRDKPEEQWWNAEDSEGKRGMIPVPYVEKYRPASASVSALIGGR"
BASE COUNT 369 a 366 c 440 g 405 t
ORIGIN
1 atttcgggcg ctgctgtgaa gctgaaaccg gagccggtcc gctgggcggc gggcgccggg
61 ggccggaggg gcgcgcgcgg cggcggcacc ccagcgttta ggcgcggagg cagccatggc
121 gggcaacttc gactcggagg agcggagtag ctggtactgg gggaggttga gtcggcagga
181 ggcggtggcg ctgctgcagg gccagcggca cggggtgttc ctggtgcggg actcgagcac
241 cagccccggg gactatgtgc tcagcgtctc agagaactcg cgcgtctccc actacatcat
301 caacagcagc ggcccgcgcc cgccggtgcc accgtcgccc gcccagcctc cgcccggggt
361 gagcccctcc agactccgaa taggagatca agagtttgat tcattgcctg ctttactgga
421 attctacaaa atacactatt tggacactac aacgttgata gaaccagttt ccaggtccag
481 gcagggtagt ggagtgattc tcaggcagga ggaggcggag tatgtgcgag ccctctttga
541 ctttaatggg aatgatgagg aagatcttcc ctttaagaaa ggagacatct tgagaatccg
601 ggacaagcct gaagagcagt ggtggaatgc ggaggacagc gaaggcaaga gagggatgat
661 tccagtccct tacgtcgaga agtatagacc tgcctccgcc tcagtatcgg ctctgattgg
721 aggtcggtga gctggtaaag gttacgaaga ttaatgtgag tggtcagtgg gaaggggagt
781 gtaatggcaa acgaggtcac ttcccattca cacatgtccg tctgctggat caacagaatc
841 ccgatgagga cttcagctga gtatagttca acagttttgc tgacagatgg gaacaatctt
901 tttttttttt ttccaactgc catctataca attttcttac agatgtcaaa agcagtctag
961 tttatataag cattctgtta cctgtgatat tttttagact gaactgctcc attcctagtc
1021 ttaattacca tattcagggt acgaactgga gggcttgtgt gttagcttct gaattggcaa
1081 ttggaggcgg tagtggtcgt gcctgtgtgt atcagaaggg ataggtatct tgcctccttt
1141 ctctcaggca gtgcaaatca ccctgtggaa aaccgatgga caggaaggag tgttacacac
1201 tgcttaccct gatttattca gtggttttgt tttcattctg gaaccatact atcaaatggc
1261 gacagactgt tccgttccac ccccgtgaag taatcatgca ccgtgtgaat agtatcaagc
1321 aggattgctt tcattgtatg gagcatgacc agcgtgtgac tcattctgac atttcagatc
1381 ctaagaattc taagaacact actagaagca tttgttccct cctagtcaat gcttcatact
1441 ttttcttggg attcttttag cccttgacat tcttgtcccc caaacctgta agtaggtgaa
1501 ttcctaagat aagtgtgtat tttcattcca ggtgaaaagc aggatgtacc gagcacttta
1561 ttcagtgcat agctttaagc
//