LOCUS AK291032 3982 bp mRNA linear HUM 09-JAN-2008
DEFINITION Homo sapiens cDNA FLJ76803 complete cds.
ACCESSION AK291032
VERSION AK291032.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3982)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..3982
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RI3005246"
/clone_lib="NT2RI3"
/db_xref="H-InvDB:HIT000423829"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells treated
2-weeks mitotic inhibitor after 5-weeks retinoic acid
(RA) induction."
/note="majorly NT2 neuron"
/organism="Homo sapiens"
CDS 153..3632
/codon_start=1
/protein_id="BAF83721.1"
/transl_table=1
/translation="MLDFLAENNLCGQAILRIVSCGNAIIAELLRLSEFIPAVFRLKD
RADQQKYGDIIFDFSYFKGPELWESKLDAKPELQDLDEEFRENNIEIVTRFYLAFQSV
HKYIVDLNRYLDDLNEGVYIQQTLETVLLNEDGKQLLCEALYLYGVMLLVIDQKIEGE
VRERMLVSYYRYSAARSSADSNMDDICKLLRSTGYSSQPGAKRPSNYPESYFQRVPIN
ESFISMVIGRLRSDDIYNQVSAYPLPEHRSTALANQAAMLYVILYFEPSILHTHQAKM
REIVDKYFPDNWVISIYMGITVNLVDAWEPYKAAKTALNNTLDLSNVREQASRYATVS
ERVHAQVQQFLKEGYLREEMVLDNIPKLLNCLRDCNVAIRWLMLHTADSACDPNNKRL
RQIKDQILTDSRYNPRILFQLLLDTAQFEFILKEMFKQMLSEKQTKWEHYKKEGSERM
TELADVFSGVKPLTRVEKNENLQAWFREISKQILSLNYDDSTAAGRKTVQLIQALEEV
QEFHQLESNLQVCQFLADTRKFLHQMIRTINIKEEVLITMQIVGDLSFAWQLIDSFTS
IMQESIRVNPSMVTKLRATFLKLASALDLPLLRINQANSPDLLSVSQYYSGELVSYVR
KVLQIIPESMFTSLLKIIKLQTHDIIEVPTRLDKDKLRDYAQLGPRYEVAKLTHAISI
FTEGILMMKTTLVGIIKVDPKQLLEDGIRKELVKRVAFALHRGLIFNPRAKPSELMPK
LKELGATMDGFHRSFEYIQDYVNIYGLKIWQEEVSRIINYNVEQECNNFLRTKIQDWQ
SMYQSTHIPIPKFTPVDESVTFIGRLCREILRITDPKMTCHIDQLNTWYDMKTHQEVT
SSRLFSEIQTTLGTFGLNGLDRLLCFMIVKELQNFLSMFQKIILRDRTVQDTLKTLMN
AVSPLKSIVANSNKIYFSAIAKTQKIWTAYLEAIMKVGQMQILRQQIANELNYSCRFD
SKHLAAALENLNKALLADIEAHYQDPSLPYPKEDNTLLYEITAYLEAAGIHNPLNKIY
ITTKRLPYFPIVNFLFLIAQLPKLQYNKNLGMVCRKPTDPVDWPPLVLGLLTLLKQFH
SRYTEQFLALIGQFICSTVEQCTSQKIPEIPADVVGALLFLEDYVRYTKLPRRVAEAH
VPNFIFDEFRTVL"
BASE COUNT 1205 a 851 c 823 g 1103 t
ORIGIN
1 gtgcgccgtg gcgcggcccg gctgacaggt tctttaatgg aggagccaat ctctctgcac
61 acctggtttc atctaataat atacagacac cagctctgag gccagttaat catccccagt
121 gtccaggcac agagtagtcg gtccgcctca caatgttgga ctttctagcc gagaacaacc
181 tctgtggcca agcaatccta aggattgttt cctgtggtaa tgccatcatt gctgaacttt
241 tgagactctc tgagtttatt cctgctgtgt tcaggttaaa agacagagct gatcaacaga
301 aatatggaga tatcatattt gatttcagct attttaaggg tccagaatta tgggaaagca
361 aactggatgc taagccagag ctacaggatt tagatgaaga atttcgtgaa aacaacatag
421 aaattgtgac cagattttat ttagcatttc aaagtgtaca taaatatatt gtagacttaa
481 acagatatct agatgatctc aatgaagggg tttatattca gcaaacctta gaaactgtgc
541 ttctcaatga agatggaaaa caacttctat gtgaagcact gtacttatat ggagttatgc
601 tactggtcat tgaccaaaag attgaaggag aagtcagaga gaggatgctg gtttcttact
661 accgatacag tgctgctcga tcttctgctg attcaaatat ggacgatatt tgcaagctgc
721 ttcgaagtac aggttattct agccaaccgg gtgccaaaag accatccaac tatcccgaga
781 gctatttcca gagagtgcct atcaacgaat ccttcatcag tatggtcatt ggtcgactga
841 gatctgatga tatttacaac caggtctcag cgtatccttt gccggagcat cgcagcacag
901 ccctggcaaa ccaagctgcc atgctgtacg tgattctcta ctttgagcct tccatccttc
961 acacccatca agcaaaaatg agagagatag tggataaata ctttccagat aattgggtaa
1021 ttagtattta catggggatc acagttaatc tagtagatgc ttgggaacct tacaaagctg
1081 caaaaactgc tttaaataat accctggacc tttcaaatgt cagagaacag gcaagcagat
1141 atgctactgt cagtgaaaga gtgcatgctc aagtgcagca atttctaaaa gaaggttatt
1201 taagggagga gatggttctg gacaatatcc caaagcttct gaactgcctg agagactgca
1261 atgttgccat ccgatggctg atgcttcata cagcagactc agcctgtgac ccaaacaaca
1321 aacgccttcg tcaaatcaag gaccagattc taacagactc tcggtacaat cccaggatcc
1381 tcttccagct gctgttagat actgcacaat ttgagtttat actcaaagag atgttcaagc
1441 aaatgctttc agaaaagcaa accaaatggg agcattacaa gaaagagggt tcggagcgga
1501 tgactgagct tgctgatgtc ttttcaggag tgaaacccct aaccagagtg gagaaaaatg
1561 aaaaccttca agcttggttc agagagatct caaaacaaat attgtcttta aattatgatg
1621 attctactgc tgcgggcaga aaaactgtac aactgataca agctttggaa gaggttcaag
1681 aattccacca gttggaatcc aatctgcaag tatgtcagtt tcttgccgat actcgaaagt
1741 ttcttcatca aatgatcaga accattaaca ttaaagagga ggttctgatc acaatgcaga
1801 tcgttgggga cctttctttc gcttggcagt tgattgacag tttcacatcc atcatgcaag
1861 aaagcataag ggtaaatcca tccatggtta ctaaactcag agctaccttc ctaaagcttg
1921 cctctgccct cgatctgccc cttcttcgta ttaatcaggc aaatagcccc gacctgctca
1981 gcgtgtcaca gtactattct ggagagttgg tatcctatgt gagaaaagtt ttgcagatca
2041 tcccagaaag catgtttaca tctcttctaa agatcataaa gcttcagacc cacgacatta
2101 ttgaagtgcc tacccgcctg gacaaagaca agctgaggga ctatgctcag ctaggcccac
2161 gatacgaggt tgccaagctt actcatgcta tttccatttt tactgaaggc atcttaatga
2221 tgaaaacgac tttggttggc atcatcaagg tggatccaaa gcagttgctg gaagatggaa
2281 taaggaaaga gcttgtgaag cgcgttgcct ttgccctgca taggggactg atattcaacc
2341 ctcgagccaa gccaagtgaa ttgatgccca agctgaaaga gttgggagcg accatggatg
2401 gattccatcg ttcttttgaa tacatacagg actatgtcaa catttatggt ctgaagattt
2461 ggcaggaaga agtatctcgt atcataaatt acaacgtgga gcaagagtgt aataactttc
2521 taagaacgaa gattcaagat tggcaaagca tgtaccagtc cactcatatt ccaataccca
2581 agtttacccc tgtggatgag tctgtaacgt ttattggtcg actctgcaga gaaatcctgc
2641 ggatcacaga cccaaaaatg acatgtcaca tagaccagct gaacacttgg tatgatatga
2701 aaactcatca ggaagtgacc agcagccgcc tcttctcaga aatccagacc accttgggaa
2761 cctttggtct aaatggctta gacaggcttc tgtgctttat gattgtaaaa gagttacaga
2821 atttcctcag tatgtttcag aaaattatcc tgagagacag aactgttcag gacactttaa
2881 aaaccctcat gaatgctgtc agtcccctaa aaagtattgt cgcaaattca aataaaattt
2941 atttttccgc cattgccaaa acacagaaga tttggactgc gtatctcgag gctataatga
3001 aggttgggca gatgcagatt ctgagacaac agattgccaa tgaattaaat tattcttgtc
3061 ggtttgattc taaacatctg gcagctgctc tggagaatct caataaggct ctcctagcag
3121 acattgaagc ccactatcag gacccttcac ttccttaccc caaagaagat aacacacttt
3181 tatatgaaat cacagcctat ctggaggcag ctggcattca caacccactg aataagatat
3241 acataacaac aaagcgctta ccctattttc caattgtaaa ctttctattt ttgatcgctc
3301 agttgccaaa acttcaatac aacaaaaatc tgggaatggt ctgccgaaaa ccgaccgacc
3361 cggttgattg gccaccactt gtcctgggac tgctcactct gctgaagcag ttccattccc
3421 ggtacaccga gcagttcctg gcgctgattg gccagtttat ctgctccacg gtggagcagt
3481 gtacaagcca gaagatacct gaaattcctg cagatgttgt gggtgccctt ctgttcctgg
3541 aggattatgt tcggtacaca aagctaccca ggagggttgc tgaagcacat gtgcctaatt
3601 tcatttttga tgagttcaga acagtgctgt aactgttttt cctacttctt caatggaagg
3661 attgtcctta gatcttccca ccatcacaaa tgaatttgaa gatgaaaaga aactcagttg
3721 ctcatacaac tgcatttttt ctgtctatta tgggaaacat cagacgttct gagtaagata
3781 tatctcatgg cattagttaa tataactgat attgtttaaa tcatggtatt acatgcaatt
3841 tatatcagat aaaagcagaa cacatttttg tactgcctct cttaaatgct gaatgtaact
3901 gttatgtata aatccattta gttttatgtt ctaaagaact atttgtgcaa ctccagattt
3961 tcagtaaaat agtattacta gt
//