LOCUS AK291027 3360 bp mRNA linear HUM 09-JAN-2008
DEFINITION Homo sapiens cDNA FLJ77466 complete cds.
ACCESSION AK291027
VERSION AK291027.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3360)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..3360
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RI3004278"
/clone_lib="NT2RI3"
/db_xref="H-InvDB:HIT000423824"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells treated
2-weeks mitotic inhibitor after 5-weeks retinoic acid
(RA) induction."
/note="majorly NT2 neuron"
/organism="Homo sapiens"
CDS 722..3073
/codon_start=1
/protein_id="BAF83716.1"
/transl_table=1
/translation="MSGGHQLQLAALWPWLLMATLQAGFGRTGLVLAAAVESERSAEQ
KAIIRVIPLKMDPTGKLNLTLEGVFAGVAEITPAEGKLMQSHPLYLCNASDDDNLEPG
FISIVKLESPRRAPRPCLSLASKARMAGERGASAVLFDITEDRAAAEQLQQPLGLTWP
VVLIWGNDAEKLMEFVYKNQKAHVRIELKEPPAWPDYDVWILMTVVGTIFVIILASVL
RIRCRPRHSRPDLLQQRTAWAISQLATRRYQASCRQARGEWPDSGSSCSSAPVCAICL
EEFSEGQELRVISCLHEFHRNCVDPWLHQHRTCPLCMFNITEGDSFSQSLGPSRSYQE
PGRRLHLIRQHPGHAHYHLPAAYLLGPSRSAVARPPRPGPFLPSQEPGMGPRHHRFPR
AAHPRAPGEQQRLAGAQHPYAQGWGLSHLQSTSQHPAACPVPLRRARPPDSSGSGESY
CTERSGYLADGPASDSSSGPCHGSSSDSVVNCTDISLQGVHGSSSTFCSSLSSDFDPL
VYCSPKGDPQRVDMQPSVTSRPRSLDSVVPTGETQVSSHVHYHRHRHHHYKKRFQWHG
RKPGPETGVPQSRPPIPRTQPQPEPPSPDQQVTRSNSAAPSGRLSNPQCPRALPEPAP
GPVDASSICPSTSSLFNLQKSSLSARHPQRKRRGGPSEPTPGSRPQDATVHPACQIFP
HYTPSVAYPWSPEAHPLICGPPGLDKRLLPETPGPCYSNSQPVWLCLTPRQPLEPHPP
GEGPSEWSSDTAEGRPCPYPHCQVLSAQPGSEEELEELCEQAV"
BASE COUNT 748 a 974 c 913 g 725 t
ORIGIN
1 atatgctgtt tggagaaaaa aagaactttt gttagcaagg aggtttcaga aatgattttg
61 gattttctgt aagtgtttaa tttagttcta ggggacagca tctctcatcc cggagtaaat
121 ttctgccttt gacctgcatg gattattttt tcaggctgcg gaatttctcg gcacctacct
181 gtagtatggg gcacttggtt tggttgcaga gtaagaaggt ggaagaatga gctgtacttg
241 gttaagcagt tgaaaccttt tttgagcagg atctgtaaaa gcataattga atttgtttca
301 cccccgtgga ttccagtggg cccgacagcg caacagtgcc tggcaacttg atgcatatgg
361 aagagcaatg ccaagtgatc tgacataata caaattcacg aagtgacatt caatcacaag
421 caaagttgga aattccaaag agaagtggtg agatctttac tagtcacagt gaagatggga
481 gaaaatgaca tacctgcagc agatgtgggc tgaaaatatc ctcttctctg cccaatcagg
541 aatgctacct gtttttggga ataaacttta gagaaaggaa gggccaaaac tacgacttgg
601 ctttctgaaa cggaagcata aatgttcttt tcctccattt gtctggatct gaggacctgc
661 atttggtatt agctagtgga agcagtatgt atggttgaag tgcattgctg cagctggtag
721 catgagtggt ggccaccagc tgcagctggc tgccctctgg ccctggctgc tgatggctac
781 cctgcaggca ggctttggac gcacaggact ggtactggca gcagcggtgg agtctgaaag
841 atcagcagaa cagaaagcta ttatcagagt gatccccttg aaaatggacc ccacaggaaa
901 actgaatctc actttggaag gtgtgtttgc tggtgttgct gaaataactc cagcagaagg
961 aaaattaatg cagtcccacc cgctgtacct gtgcaatgcc agtgatgacg acaatctgga
1021 gcctggattc atcagcatcg tcaagctgga gagtcctcga cgggcccccc gcccctgcct
1081 gtcactggct agcaaggctc ggatggcggg tgagcgagga gccagtgctg tcctctttga
1141 catcactgag gatcgagctg ctgctgagca gctgcagcag ccgctggggc tgacctggcc
1201 agtggtgttg atctggggta atgacgctga gaagctgatg gagtttgtgt acaagaacca
1261 aaaggcccat gtgaggattg agctgaagga gcccccggcc tggccagatt atgatgtgtg
1321 gatcctaatg acagtggtgg gcaccatctt tgtgatcatc ctggcttcgg tgctgcgcat
1381 ccggtgccgc ccccgccaca gcaggccgga tctgcttcag cagagaacag cctgggccat
1441 cagccagctg gccaccagga ggtaccaggc cagctgcagg caggcccggg gtgagtggcc
1501 agactcaggg agcagctgca gctcagcccc tgtgtgtgcc atctgtctgg aggagttctc
1561 tgaggggcag gagctacggg tcatttcctg cctccatgag ttccatcgta actgtgtgga
1621 cccctggtta catcagcatc ggacttgccc cctctgcatg ttcaacatca cagagggaga
1681 ttcattttcc cagtccctgg gaccctctcg atcttaccaa gaaccaggtc gaagactcca
1741 cctcattcgc cagcatcccg gccatgccca ctaccacctc cctgctgcct acctgttggg
1801 cccttcccgg agtgcagtgg ctcggccccc acgacctggt cccttcctgc catcccagga
1861 gccaggcatg ggccctcggc atcaccgctt ccccagagct gcacatcccc gggctccagg
1921 agagcagcag cgcctggcag gagcccagca cccctatgca caaggctggg gactgagcca
1981 cctccaatcc acctcacagc accctgctgc ttgcccagtg cccctacgcc gggccaggcc
2041 ccctgacagc agtggatctg gagaaagcta ttgcacagaa cgcagtgggt acctggcaga
2101 tgggccagcc agtgactcca gctcagggcc ctgtcatggc tcttccagtg actctgtggt
2161 caactgcacg gacatcagcc tacagggggt ccatggcagc agttctactt tctgcagctc
2221 cctaagcagt gactttgacc ccctagtgta ctgcagccct aaaggggatc cccagcgagt
2281 ggacatgcag cctagtgtga cctctcggcc tcgttccttg gactcggtgg tgcccacagg
2341 ggaaacccag gtttccagcc atgtccacta ccaccgccac cggcaccacc actacaaaaa
2401 gcggttccag tggcatggca ggaagcctgg cccagaaacc ggagtccccc agtccaggcc
2461 tcctattcct cggacacagc cccagccaga gccaccttct cctgatcagc aagtcaccag
2521 atccaactca gcagcccctt cggggcggct ctctaaccca cagtgcccca gggccctccc
2581 tgagccagcc cctggcccag ttgacgcctc cagcatctgc cccagtacca gcagtctgtt
2641 caacttgcaa aaatccagcc tctctgcccg acacccacag aggaaaaggc gggggggtcc
2701 ctccgagccc acccctggct ctcggcccca ggatgcaact gtgcacccag cttgccagat
2761 ttttccccat tacaccccca gtgtggcata tccttggtcc ccagaggcac accccttgat
2821 ctgtggacct ccaggcctgg acaagaggct gctaccagaa accccaggcc cctgttactc
2881 aaattcacag ccagtgtggt tgtgcctgac tcctcgccag cccctggaac cacatccacc
2941 tggggagggg ccttctgaat ggagttctga caccgcagag ggcaggccat gcccttatcc
3001 gcactgccag gtgctgtcgg cccagcctgg ctcagaggag gaactcgagg agctgtgtga
3061 acaggctgtg tgagatgttc aggcctagct ccaaccaaga gtgtgctcca gatgtgtttg
3121 ggccctacct ggcacagagt cctgctcctg ggaaaggaaa ggaccacagc aaacaccatt
3181 ctttttgccg tacttcctag aagcactgga agaggactgg tgatggtgga gggtgagagg
3241 gtgccgtttc ctgctccagc tccagacctt gtctgcagaa aacatctgca gtgcagcaaa
3301 tccatgtcca gccaggcaac cagctgctgc ctgtggcgtg tgtgggctgg atcccttgaa
//