LOCUS AK291025 1694 bp mRNA linear HUM 09-JAN-2008
DEFINITION Homo sapiens cDNA FLJ76174 complete cds, highly similar to Homo
sapiens SH3 domain protein D19 (EVE1), mRNA.
ACCESSION AK291025
VERSION AK291025.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1694)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..1694
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RI3004076"
/clone_lib="NT2RI3"
/db_xref="H-InvDB:HIT000423822"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells treated
2-weeks mitotic inhibitor after 5-weeks retinoic acid
(RA) induction."
/note="majorly NT2 neuron"
/organism="Homo sapiens"
CDS 289..1551
/codon_start=1
/note="highly similar to Homo sapiens SH3 domain protein
D19 (EVE1), mRNA"
/protein_id="BAF83714.1"
/transl_table=1
/translation="MDLQKKQSNLATGLSKAKSQVFKNQDPVLPPRPKPGHPLYSKYM
LSVPHGIANEDIVSQNPGELSCKRGDVLVMLKQTENNYLECQKGEDTGRVHLSQMKII
TPLDEHLRSRPNDPSHAQKPVDSGAPHAVVLHDFPAEQVDDLNLTSGEIVYLLEKIDT
DWYRGNCRNQIGIFPANYVKVIIDIPEGGNGKRECVSSHCVKGSRCVARFEYIGEQKD
ELSFSEGEIIILKEYVNEEWARGEVRGRTGIFPLNFVEPVEDYPTSGANVLSTKVPLK
TKKEDSGSNSQVNSLPAEWCEALHSFTAETSDDLSFKRGDRIQILERLDSDWCRGRLQ
DREGIFPAVFVRPCPAEAKSMLAIVPKGRKAKALYDFRGENEDELSFKAGDIITELES
VDDDWMSGELMGKSGIFPKNYIQFLQIS"
BASE COUNT 522 a 352 c 394 g 426 t
ORIGIN
1 agtccacctc ctctccctgc tgaaaaacct attggaaaca ctttcagtac agtatctgga
61 aagctcagta atgttgagag aactagaaac ttggaatcca accacccagg tcaaacagga
121 ggttttgtgc gagtaccccc aaggttgcca ccgagacctg tgaatggaaa aaccattcca
181 actcaacagc ctccaaccaa ggtgccccct gagagaccac ctcccccaaa gctttctgca
241 accagaagat ctaataagaa actgcctttt aatcgatcct cttctgacat ggatcttcag
301 aaaaaacaaa gtaacttggc aactggactc tcaaaagcca agagtcaagt ttttaaaaat
361 caagatccgg tgctaccccc tcgtcccaaa ccaggacacc ctctctacag taaatacatg
421 ctgtctgtgc ctcatggaat tgccaatgaa gatattgtct ctcaaaaccc cggagaactc
481 tcttgtaagc gtggggatgt acttgtgatg ctgaagcaga cggaaaataa ttacttggag
541 tgccaaaagg gagaagacac tggcagagtt cacctgtctc aaatgaagat tatcactcca
601 cttgatgaac atcttagaag cagaccaaac gatccaagcc acgctcagaa gcctgttgac
661 agtggtgctc ctcatgctgt cgttcttcat gatttcccag cagagcaagt tgatgatttg
721 aacctcactt ctggagaaat tgtttatctt ctggagaaga tagatacaga ttggtacaga
781 gggaactgta gaaaccagat tggcatattt cctgccaact atgtcaaagt gattattgat
841 atcccagaag gaggaaatgg gaaaagagaa tgtgtttcat ctcattgtgt taaaggctca
901 agatgtgttg ctcggtttga atatattgga gagcagaagg atgagttgag tttctcagag
961 ggagaaatta ttattcttaa agagtatgtg aatgaggaat gggccagagg agaagttcga
1021 ggcagaactg ggattttccc cctgaacttt gtggagcctg ttgaggatta tcccacctct
1081 ggtgcaaatg ttttaagcac aaaggtacca ctgaaaacca aaaaagaaga ttctggctca
1141 aactctcagg ttaacagtct tccggcagaa tggtgtgaag ctcttcacag ttttacagca
1201 gagaccagtg atgacttatc attcaagagg ggagaccgga tccagattct ggaacgtctg
1261 gattctgact ggtgcagggg cagactgcag gacagggagg ggatcttccc agcagtgttt
1321 gtgaggccct gcccagctga ggcaaaaagt atgttggcca tagtaccgaa ggggaggaag
1381 gccaaagcct tatatgattt ccgaggggag aatgaagatg aactttcctt caaggctgga
1441 gatataataa cagagctgga atctgtagat gatgactgga tgagtggaga acttatggga
1501 aaatctggaa tatttcccaa aaactacata cagtttctac agatcagcta gaggagaagc
1561 ttgtctgtgt tccttggcac aagaactcac ttgaactatc accttgacta tcagatatgt
1621 ttttgcacta ttttttttaa ctgaaaaaga aatatctaag ctgtacatgg tacactagaa
1681 ttttctgaaa gcag
//